Labshake search
Citations for Biotium Inc. :
951 - 1000 of 1910 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2023Quote: ... and CF568 conjugated-donkey anti-mouse IgG (Biotium, Hayward) were used as secondary antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
-
bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
-
bioRxiv - Genetics 2023Quote: ... The gel was stained with 0.1 uL Gel Red (Biotium, CA, USA) visualized under UV illumination at 300 nm using the Enduro GDS Touch Imaging System (Labnet International ...
-
bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
-
bioRxiv - Microbiology 2023Quote: ... Photoactivation was performed by using the PMA-LiteTM LED Photolysis Device (Biotium) with the exposure time set to 30 min ...
-
bioRxiv - Genomics 2023Quote: ... The DNA samples were quantified before fragmentation reactions using Qubit and an AccuGreen Broad Range dsDNA Quantitation Kit (Biotium).
-
bioRxiv - Genomics 2023Quote: ... cells were washed with DPBS and lysed directly in Qiazol reagent using a sterile miniature cell scraper (Biotium). Cell lysate was collected and stored for subsequent RNA processing and analysis.
-
bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
-
bioRxiv - Immunology 2023Quote: ... cells were stained in DMEM 1% penicillin/streptomycin with 5 nM MitoView 533 (Biotium, #70055). Plates were incubated at 37°C for 30 min protected from light ...
-
bioRxiv - Immunology 2023Quote: ... qPCR was performed with the Fast EvaGreen qPCR Master Mix (Biotium) and measured on a ABI7300 Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... 2.5X EvaGreen (Biotium, Fremont, CA), 0.02 U/µl Phusion polymerase (New England Biolabs ...
-
bioRxiv - Microbiology 2023Quote: ... or Resazurin Cell Viability Assay Kit (Biotium #30025-1). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... and the assay was performed using the Griess Reagent Kit (Biotium) following the guidelines as provided in the kit ...
-
bioRxiv - Microbiology 2023Quote: ... and used in a qPCR using a ready-to-use mix containing a fluorescent-intercalating dye (EvaGreen® Fast qPCR Master Mix, Biotium, San Francisco, USA). After the amplification steps ...
-
bioRxiv - Microbiology 2023Quote: ... 1X (Biotium, Fremont, CA, USA) as per manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
-
bioRxiv - Cell Biology 2023Quote: ... Fixed cells were additionally stained with membrane marker CellBrite (dilution 1:200, Biotium, Fremont, CA, USA) and actin marker Phalloidin (dilution 1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were incubated in blocking solution containing Phallodin-CF680R (1:50, Biotium #00048) for 20 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Calcein AM (Biotium, Fremont ...
-
bioRxiv - Developmental Biology 2023Quote: ... and renilla (Biotium 300821) luciferase assays according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... centrifuged for 10 min at ∼13,000×g and then 50 µl of the clear supernatant lysate was used separately in firefly (Biotium #30085-1) and renilla (Biotium 300821 ...
-
bioRxiv - Cell Biology 2023Quote: ... and Hoechst 33342 (1:500; Biotium) to visualize specific proteins (i.e ...
-
bioRxiv - Cell Biology 2023Quote: ... and Cellbrite Steady 550 with Cellbrite Enhancer reagent (Biotium, 30107-T) by first making a 10X solution by diluting the three reagents 1:100 in R10 medium ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-POL1RE was linked to CF488 (92273, Biotium), anti-MKi67IP was linked to CF594 (92276 ...
-
bioRxiv - Cell Biology 2023Quote: ... primary antibodies were first labelled with fluorescent dyes using the Mix-n-Stain antibody labelling kit (Biotium) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice with 1X PBS and then incubated with TrueBlack IF Background Suppressor (Biotium) for 30 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with secondary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 1 hour at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 2 hours at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... conjugated to CF555 (Mix-n-Stain CF Antibody Labeling Kit, Biotium, Fremont, CA, USA)
-
bioRxiv - Genetics 2023Quote: ... and 0.5 mg/mL BSA (22014, BIOTIUM). Flow cytometry was performed to measure mCitrine ...
-
bioRxiv - Cell Biology 2023Quote: ... Phalloidin-CF405M was purchased from Biotium (Cat. No. 00034; 1:200) and Phalloidin-Atto488 was purchased from Bioreagent (Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... Optical configurations were adjusted to capture the CellBrite Steady 550 Membrane Stain (#30107, Biotium), and the reflection from the grids in the far-red channel with excitation wavelengths of 555 and 639 nm ...
-
bioRxiv - Cell Biology 2023Quote: ... The pellet was resuspended in 100 µL CellBrite Steady Membrane Stain 550 (#30107, Biotium, Fremont, CA, USA) in HBSS (1:1000 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and stained post-electrophoresis with GelRed® (Biotium). pif expression was assessed with the Luna® Universal Two-Step RT-qPCR Master Mix (New England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted onto the slides with EverbriteTM Fluorescence Antifade Mounting Medium (Quartzy/Biotium, 23002) containing DAPI as a nuclear stain ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... and mounted in EverBrite Hardset Mounting medium (Biotium). Super-resolution structured illumination microscopy (SR-SIM ...
-
bioRxiv - Developmental Biology 2023Quote: ... TMRM (Biotium) was then added at 100 nM to the previous mix for 15 min at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... TrueBlack (Biotium #23007) was applied to quench background fluorescence ...
-
bioRxiv - Biochemistry 2023Quote: ... Membranes were blocked in 1X TBST and 1X fish gelatin (Biotium, Fremont, CA) and blotted with E-12 mouse monoclonal anti-human CIITA (catalog number sc-376174 ...
-
bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 5µM NucView 488 caspase-3 substrate (Biotium, 30029) and media to 50µl was added ...
-
bioRxiv - Bioengineering 2023Quote: ... These cross-sectioned samples were washed twice with PBS to remove the culture media before being incubated in a live/dead assay staining solution (obtained from Biotium, Hayward, CA, USA) for a duration of 30 minutes ...
-
bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Bioengineering 2023Quote: ... The samples were incubated with 25 µg/mL wheat germ agglutinin CF™594 conjugate (Biotium; Excitation/Emission: 593/614 nm) diluted with NaCl buffer for 30 minutes at room temperature and subsequently washed with the NaCl buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... This was achieved by application of 100 nM TTX (tetrodotoxin citrate, Biotium) in a 750 mM sucrose (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... and Tetramethylrhodamine ethyl ester perchlorate (TMRE) (Biotium, Fremont, CA).
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568-conjugated phalloidin (Biotium) and DAPI (Biotium ...
-
bioRxiv - Cell Biology 2023Quote: ... The number of cycles for each PCR reaction was optimized by qPCR with EvaGreen (Biotium, 31000) on a CFX Opus 96 Real-Time PCR System (Bio-Rad).