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Citations for VWR :
51 - 100 of 301 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... After PCR amplification using NEBNext High-Fidelity 2 × PCR Master Mix (VWR 102500-096) and post-PCR cleanup using SPRI beads (Beckman Coulter A63880) ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed three times 1X PBS (VWR, life science) and resuspended in 1 ml 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of freshly prepared 2M Urea (VWR) resuspended in 10mM Tris-HCl pH 8.0 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... difficile strains containing the phiLOV2.1 reporter plasmid were grown anaerobically at 37°C in BHIS-Tm10 for 12 - 16 h and inoculated 1:50 into fresh BHIS-Tm10 containing indicated treatments (1.70 µg mL−1 vancomycin (VWR), 0.67 µg mL−1 fidaxomicin (Cayman Chemical) ...
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bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were serum-starved 16 hours prior to plating with 1% bovine serum albumin (VWR, Cat. no. 0332-100G) added for protein supplementation ...
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bioRxiv - Bioengineering 2021Quote: ... mixed with 300 μl of digestion buffer in a new Eppendorf tube and placed at 60°C (16 hrs, Thermormixer Compact F 1.6 A,VWR) for digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were carefully dissected before immersion fixation for 16-24 hours in 10% neutral buffered formalin (VWR, Radnor, PA) and cryoprotection in 30% sucrose for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
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bioRxiv - Microbiology 2021Quote: ... and visualization was performed using the AlkPhos Direct Labeling and Detection System (VWR 95038-288) and CDP-Star reagent (VWR 95038-292) ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were imaged by incubating them with the Amersham ECL western blotting detection reagent (VWR) for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1100 rpm for 16 hours using a Mini Shaking Incubator (VWR Catalog # 12620-942, VWR International LLC., Radnor, PA, USA.). 100 μL of sterile 20% glycerol was added to 100 μL of overnight culture to prepare glycerol stock plate.
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bioRxiv - Neuroscience 2020Quote: ... The olfactory epithelium was cryosectioned coronally into 16 μm -thick sections mounted on Superfrost Plus slides (VWR, West Chester, PA) coated with poly-D-lysine.
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bioRxiv - Cell Biology 2022Quote: ... HAF were seeded onto Ø 10 or 16 mm borosilicate glass cover glasses (631-0170 and 631-0151, VWR, Germany). After transfer into 12- or 24-well plates (665102 and 662160 ...
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bioRxiv - Microbiology 2022Quote: ... hexadec-9-enoic acid (P9417, MilliporeSigma), octadecanoic acid (S4751, MilliporeSigma), and pentadecanoic acid (P6125, MilliporeSigma) in ethanol (MACR6777-16, VWR), and 20% tyloxapol (T8761 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2 times with Histochoice Clearing Agent (VWR, H103) and 4 times with isopropanol (VWR,9084-03 ...
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bioRxiv - Biochemistry 2021Quote: ... PKCδ was visualized using the Amersham Enhanced Chemiluminescence Western blotting detection system (VWR, Mississauga, ON, Canada). Band intensity was quantified using Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times with PBS with 0.1% Tween 20 (VWR International AB) (PBST) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were dounced 20 times in a 7ml stainless tissue grinder (VWR) and then spun at 200g for 5min to pellet large debris ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT before being mounted on a microscope slide using VECTASHIELD (VWR, #H-1000) and sealed in nail polish ...
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bioRxiv - Immunology 2023Quote: ... into empty 96-well PCR plates (VWR). FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2020Quote: ... Cryosections were cut at a thickness of 10-16 µm using a Leica CM3050 S cryostat and placed onto SuperFrost®Plus™ slides (VWR). Prior to staining ...
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bioRxiv - Immunology 2022Quote: ... region of the 16S rRNA gene was performed in 20 uL duplicate reactions: 8 uL of 2.5X 5Prime Hotstart Mastermix (VWR, Radnor, PA, USA), 1 uL of 20X Evagreen (VWR) ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Molecular Biology 2022Quote: ... for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent, VWR).
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bioRxiv - Developmental Biology 2022Quote: ... Stained slides were washed 3 times in PBS and No.1 coverslips (VWR) were added using ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then disrupted 5 times with a dounce homogenizer (VWR cat. no. 62400-595). Following mechanical disruption ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed 4 times with 0.1% NP-40 substitute (VWR, 97064-730) in tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was washed three times and TMB (VWR International, 50-76-03) substrate was added ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Neuroscience 2023Quote: ... 16 µm NAcc coronal sections were obtained with a Leica CM3050S cryostat and mounted onto SuperFrost Plus glass slides (VWR, Radnor, PA, USA). The sections were collected in a way that each slide contained rostral ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Zoology 2020Quote: ... PCR reactions were performed in a 55 μl volumes containing: 25 μl Hot Start PCR-to-Gel Master Mix (VWR brand); 2 μl of primer mix (25 pmol each of forward and reverse primer) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Immunology 2023Quote: ... All samples were diluted ten times in low EDTA TE-buffer (VWR, cat.: APLIA8569.0500) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... an adapter PCR reaction was performed as follows: All 8 tubes of a 50 µL PCR strip tube (VWR, catalog # 490003-606) were filled with 2500 ng DNA template ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed 5x with TBST and signal was detected using ECL Western Blotting detection reagent (GE) or ECL prime (VWR).
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Microbiology 2020Quote: Samples were incubated with 10 mM CCCP (prepared in 50% DMSO) for 30 min at RT followed by addition of 10 mM Nile Red (VWR) at 37°C and shaken at 200 rpm for 30 min ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The membranes were washed 3x in PBST at RT and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (VWR, Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated on ice in 96-well PCR plates (VWR) for 30 min and then heat-shocked for 1 min at 42 °C in a BioRad T100 Thermal cycler ...
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bioRxiv - Biophysics 2021Quote: ... in a 384-well PCR microplate (VWR, 10011-194), with a final volume of 15 μL per well ...
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bioRxiv - Synthetic Biology 2020Quote: ... in RNase-free 0.2 ml PCR tubes (VWR International) or 1.5 ml DNA LoBind tubes (Eppendorf) ...