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Citations for VWR :
1 - 50 of 301 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ...
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bioRxiv - Immunology 2024Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) using primers 154-CP-probe-F & 155-CP-probe-R ...
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bioRxiv - Immunology 2024Quote: ... and RT-PCR was performed with RedTaq Polymerase (VWR) using primers 340-CP-Taq-F and 264-qRel-R2 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2020Quote: ... three times at RT and then incubated with Thiazine-Red (0.05%, cat#27419.123, VWR) for 20 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Genomics 2022Quote: ... 16% PEG8000 (VWR Life Science, 97061), 0.5mM ATP (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Cell Biology 2020Quote: ... ECL detection system (VWR) and SuperSignal West Dura (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Biochemistry 2022Quote: ... samples or PC(16:0/16:0) (DPPC) for the standard curve were manually shaken for 30s in a glass vial (VWR) with 1 mL PBS ...
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bioRxiv - Microbiology 2022Quote: ... then sonicated 16 minutes (VWR Ultrasonic Cleaner USC-T) to obtain 30nm extruded Small Unilamellar Vesicles (SUVs) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1100 rpm for 16 hours using a Mini Shaking Incubator (VWR Catalog # 12620-942 ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Biochemistry 2023Quote: ... using ECL Western Blotting Detection Reagent (VWR, RPN2106).
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... samples were fixed for 30 min in 4 % formaldehyde (16 % VWR 100503) diluted in pH 7.0 PBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Biophysics 2022Quote: ... ECL Western blotting detection reagent (GE) or ECL prime (VWR) were used for detection.
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bioRxiv - Microbiology 2022Quote: ... and developed with Amersham ECL Select chemiluminescent detection reagent (VWR) according to the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2019Quote: ... A total of 5 µL of Ant-miR-218 or Ant-Scrambled were administered per nostril with a 10XL pipette tip (VWR, Ville Mont-Royal, Quebec, Canada) attached to a P10 micropipette (Corning ...
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bioRxiv - Microbiology 2023Quote: Samples were analyzed using standard SDS-PAGE (4-16% bis acrylamide, Precast gels, VWR) and immunoblotting techniques ...
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bioRxiv - Cell Biology 2019Quote: ... Detection was carried out by using AceGlow (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cell Biology 2024Quote: ... HRP was detected using ECL western blotting detection reagents (VWR, # RPN2106). Chemiluminescence was detected using and X-ray films (Bema ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Molecular Biology 2022Quote: ... Homogenates were sonicated three times for 30s each time at room temperature in a water bath sonicator (VWR, 97043-960). Samples were rested on ice for 30s between each cycle ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of 0.1M Na2CO3 (VWR), 1 time with 1ml of freshly prepared 2M Urea (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 times with isopropanol (VWR,9084-03) and 2 times with distilled water for 3 minutes (min ...
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bioRxiv - Neuroscience 2020Quote: ... 0.5 mM glutamine and penicillin/streptomycin (Ozyme) on 60-mm dishes or 16-mm glass coverslips (VWR) pre-coated with poly-L-Lysine (0.5 mg mL-1 ...
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bioRxiv - Plant Biology 2022Quote: ... then 16 μm thick cross sections were cut and transferred to cold microscope slides (SuperFrost Plus, VWR International using cryofilm (2C ...
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bioRxiv - Developmental Biology 2023Quote: Cells were lysed after 12 h or 16 h using 500 μL pepGOLD TriFast ™ reagent (VWR) and stored at -80 until the RNA extraction process was resumed ...
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bioRxiv - Cell Biology 2019Quote: ... Activity was detected using the AceGlow blotting detection system (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... Sections were dried at RT for 30 minutes on a glass coverslip (VWR), and either stored dried at −80°C or directly processed by rehydration in 1XPBS followed by fixation in 4% PFA for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Microbiology 2020Quote: ... solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE, VWR) and 5 µl of enzyme solution ...
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bioRxiv - Cell Biology 2023Quote: ... strips were covered with Axygen PCR-tube caps (VWR, PCR-02-FCP-C), centrifuged and placed on dry ice until storage at −80°C for further use ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified using Five prime hot master mix (VWR, Australia) using primers with custom barcodes ...