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Citations for VWR :
51 - 100 of 187 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... with 1.4 mL of working buffer (0.25 M sucrose (200-334-9, VWR), 6 mM EDTA (E5134 ...
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bioRxiv - Immunology 2021Quote: ... and was stopped by addition of 2 M sulfuric acid (BCH7500-1, VWR) at 1:1 volume ratio ...
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bioRxiv - Bioengineering 2020Quote: ... and added 10 ml of a solution containing 0.2 M NaOH (VWR international) and 1% SDS ...
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bioRxiv - Developmental Biology 2019Quote: ... Cryorections of 8- m thickness were made on Superfrost Plus Micro slides (VWR) using a Leica Microsystems cryostat ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Plant Biology 2020Quote: ... using a tank blot system (Perfect Blue Web M, VWR International GmbH, Darmstadt, Germany). Immunochemical detection was performed using an enhanced chemiluminescence detection reagent (ECL Prime ...
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bioRxiv - Plant Biology 2023Quote: ... using a tank blot system (Perfect Blue Web M, VWR International GmbH, Darmstadt, Germany). Immunochemical detection was performed using an enhanced chemiluminescence detection reagent (ECL Prime ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... with flow rates ranging from 0.1-0.3 m/s (VWR-21800-024 hot wire anemometer). During conditioning bees were offered a single drop of sucrose on a yellow strips cut from plastic folders ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 μ m pore-size syringe filter with 25 mm ID (VWR International, Radnor, PA), a column equipped with 30 mg of C18 resin beads (C18 column) ...
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bioRxiv - Bioengineering 2021Quote: ... treatments were applied by transferring the plants to 0.7% (m/v) agarose (VWR Life Sciences) with 1.5 mM MES buffers (Duchefa ...
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bioRxiv - Biophysics 2021Quote: ... The sandwich consists of a bottom layer of PAA gel (E ≈ 1800 Pa) and a top layer of a 12 mm round glass cover slip (VWR).
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bioRxiv - Neuroscience 2020Quote: ... pH 7.8) supplemented with 6 M Guanidine-HCl using a pellet homogenizer (47747-370, VWR international). The lysate was boiled for 3 min and cleared by centrifugation at 20’000 g for 5 min ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... with flow rates ranging between 0.1 to 0.3 m/s(VWR-21800-024 hot wire anemometer). During testing bees were able to walk back and forth in the vials ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...
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bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
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bioRxiv - Cell Biology 2020Quote: ... Thin sections of tissue (10 ⎧m) were mounted in 24-well glass bottom plates (VWR 82050-898) coated with a 1:50 dilution of APTES (Sigma 440140) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 OD units of log phase cells (corresponding to OD600 of 0.8-1.2) grown overnight in YPD were collected and subjected to lysis [0.2 M NaOH (VWR), 0.2% β-mercap-toethanol (Sigma)] and chilled on ice for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... then washed three times with 0.1 M PB before being mounted onto Superfrost slides (VWR, Radnor, PA) with 0.1% gelatin and coverslipped with Prolong Gold antifade reagent (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... and controlled at 7.0 by addition of filter-sterilized 4.0 M KOH (0.2 µm PES filters) (VWR). The base vessel and 10-L feed vessels were connected via sterile 0.6-mm needles through septa on the head-plate (one feed vessel per reactor) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 x 107 cells per replicate were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or 2.5% (w/v) agarose plus either 1.5 M sodium chloride solution (VWR Chemicals, cat. no. 27810.364), 2 M D-Fructose solution (Sigma Aldrich cat ...
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bioRxiv - Bioengineering 2023Quote: ... Ukraine) was mixed with 50 mL ice-cold solution of HCl (12 M, 37 wt. % VWR, Germany), deionized water ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were homogenized in 1.5 mL 8 M Urea buffer using a Wheaton Potter-Elvehjem Tissue Grinder (VWR). The lysates were sonicated and centrifuged at 13000 rpm for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... Blocks of around 1 mm2 were cut and cryo-protected by overnight infiltration in 2.3 M sucrose (VWR) in 0.1 M phosphate buffer ...
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bioRxiv - Microbiology 2023Quote: ... The end of the Tongue Cleaner was placed in a sterile vessel containing 2mL phosphate-buffered saline (PBS, pH=7.4, M/L= 1.37M sodium chloride, 0.27M potassium chloride, 0.1M phosphate buffer; VWR, Lutterworth, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR ...
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bioRxiv - Microbiology 2021Quote: ... We mixed 30 μl of each lysate with 200 μl UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 μl centrifugal filter unit (VWR International) and centrifuged the mixture at 14,000 x g for 40 min ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed by placing the plates on a shaker after adding 250 μL 0.1 M NaOH (VWR, US). Aliquots of 150 μL were pipetted out of each well into a scintillation bottle (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Microbiology 2023Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... lysate and UA solution were mixed in a 3:10 ratio (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µL centrifugal filter unit (VWR International) and the mixture was centrifuged at 14,000g for 40 min ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Microbiology 2024Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... we combined 60 uL of the lysate supernatant with 400 uL UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µl centrifugal filter unit (VWR International) and centrifuged for 40 minutes ...
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Neuroscience 2020Quote: ... Warm phosphate buffer (0.1 M; 21.7 mM NaH2PO4 [Chem Supply, #SA061-500G], 81 mM Na2HPO4 [VWR Chemicals, #102494C], pH 7.2) was perfused ...
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bioRxiv - Genomics 2022Quote: ... 5μm consecutive sections to the sections used for Visium FFPE were placed onto Superfrost Plus microscope slides (#631-9483, VWR) and stored at 4°C until used for RNAScope and in situ sequencing ...