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Citations for VWR :
1 - 50 of 187 citations for Zika Virus VLP E prM M Proteins since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were eluted using 0.1 M glycine at pH 3.5 and concentrated using Vivaspin spin filters (VWR) with appropriate MW cut-offs ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Microbiology 2019Quote: ... 0.132 M K2HPO4 (VWR Lifesciences), 1mg/ml thiamine (Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 mL PBS + 1 M NaCl and 50 mL 2 M Urea (VWR, 97063-798) + 50 mM Ammonium Bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... and digested with 1 mg/mL pronase E (39052, VWR) per 1 g tissue for 6-8 h at 37 °C and 5% CO2 under constant agitation ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Bioengineering 2021Quote: ... 4.96 uL of 1 M NaOH (VWR), 49.21 μL of ultrapure water ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were diluted to 2 M urea / 0.1 M NH4HCO3 with 140 μl of HPLC grade water (VWR). Digest with mass-spec grade trypsin (Serva ...
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bioRxiv - Microbiology 2023Quote: ... 60 µl of lysate was combined with 400 µl of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in 10 kDa MWCO 500 µl centrifugal filters (VWR International). Samples were centrifuged at 14,000 g for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were thawed on ice and resuspended in 250 μL of PBS supplemented with 1 M NaCl and 6 M Urea (VWR). The resuspended bacteria were then added to screw cap microcentrifuge lysing tubes (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 μl of 4 M perchloric acid (VWR) were combined and vortexed ...
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bioRxiv - Neuroscience 2019Quote: ... or 0.3 M BDM (2,3-Butanedione monoxime) (VWR) dissolved in either S-basal or salt stimulation buffer was introduced via the buffer channel of the microfluidics system to paralyze the anterior portion of the worm ...
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bioRxiv - Microbiology 2020Quote: ... to 3 parts 0.5 M ammonia (VWR #BDH153312K) for 14 h ...
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bioRxiv - Genetics 2024Quote: ... we added 1 M of Betaine enhancer (VWR) per reaction ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... We collected 14-m cryosections on Superfrost slides (VWR) using a Leica CM3050S cryostat ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with complete Protease Inhibitor Cocktail (VWR, M-250) and PhosSTOP (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... and reconstituted in 8 M urea (VWR 97063-802). Laemmli buffer (Bio-Rad 1610747 ...
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bioRxiv - Neuroscience 2024Quote: ... Hydrochloric acid solution (1 M) was acquired from VWR international S.A.S ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 5 mM Na3 citrate 2H2O) with ∼75u/mL Pronase E (VWR, Radnor, PA) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tygon tubing was replaced by flexible pumping tubing (Masterflex Tygon E-3603, VWR). Outflow tubing were connected to outlet ports ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 M Arabinose stocks was prepared dissolving Arabinose (VWR, A11921) in milliQ water and immediately filter sterilized through a 0.2 µM filter and stored at 4 °C ...
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Pangenomic Landscapes Shape Performances of a Synthetic Genetic Circuit Across Stutzerimonas SpeciesbioRxiv - Synthetic Biology 2024Quote: ... Working stock solutions of 1 M L-Arabinose (VWR, A11921) stock and 1 mM aTc (VWR ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Developmental Biology 2022Quote: ... in 0.083 M sodium cacodylate buffer (pH 7.2) (VWR 102090-966) at 4 °C for 3-5 hrs ...
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bioRxiv - Synthetic Biology 2019Quote: ... HEPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
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bioRxiv - Bioengineering 2021Quote: ... HEPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
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bioRxiv - Microbiology 2021Quote: ... HEPES buffer solution (C8H18N2O4S, VWR, 1 M in water, pH = 7.3), potassium phosphate dibasic (K2HPO4 ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC.
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bioRxiv - Immunology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were then incubated for 20 minutes in 0.1 M glycine (VWR) with mild shaking to reduce background autofluorescence ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2023Quote: ... then treated with Pronase E (100µg/ml in Tris-HCl (pH 7.2) + 0.06% NaCl) (VWR Chemicals) for 2 hours at 60C to remove proteins covalently bound to PG ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...