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Citations for VWR :
251 - 300 of 390 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
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bioRxiv - Pathology 2024Quote: ... HRP-conjugated secondary antibodies (NA9340, VWR International GmbH, Darmstadt, Germany) and chemiluminescent substrate (WesternBright Chemilumineszenz Substrat Quantum ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Neuroscience 2019Quote: ... and blood from trunk was rapidly collected in BD vacutainer tubes containing EDTA as anticoagulant (VWR, Ville Mont-Royal, Quebec, Canada). No cervical dislocation was applied prior to decapitation to maximize the amount of blood collected as approved by our Institutional Animal Care and use Committee ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with TBS-T (20mM Tris-HCl, pH 7.6, 136mM NaCl and 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant media containing viral particles was harvested and filtered through a 0.45 µm polyethersulfone (PES) filter (VWR, Randor, Pennsylvania, US) and aliquoted ...
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bioRxiv - Immunology 2022Quote: ... Recipient mice were maintained for 2 weeks on autoclaved food and water containing 2 mg/ml neomycin sulfate (VWR 89149–866) and 1000 U/ml polymyxin B (Millipore Sigma P4932-5MU) ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 30 mins in 150 μl lysis buffer containing 8 mg/mL lysozyme (from chicken egg white, #76346-678, VWR, USA), 10 mM Tris HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: The harvested embryos were transferred to a 4 well dish containing 4% PFA (Alfa Aesar via VWR Cat. No. AA43368-9M) for 10 mins ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Immunology 2023Quote: ... brains were isolated and placed into a 50 mL conical tube (Fisher Healthcare) containing 5 mL of RPMI (VWR International LLC). The RPMI containing the brains of the mice were then transferred to a 7 mL glass Tenbroeck dounce homogenizer (Pyrex ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each bone was then cut in half and transferred to a 1.5 ml microcentrifuge tube containing a G-Tube microcentrifuge tube (VWR, Radnor, PA, USA) that had been punctured at the bottom with a 15 gauge needle ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Cancer Biology 2020Quote: 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-742). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then transferred each of the recovered cell culture to 100 mL of molten LB (1% tryptone, 0.5% yeast extract, 1% NaCl) containing 0.3% SeaPrep agarose (VWR, Radnor, PA #12001– 922) spread into a thin layer in a 1L baffled flask (about 1 cm deep) ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of each reaction were mixed with loading dye and analyzed on a 1% (w/v) agarose gel containing GelRed (VWR, Darmstadt, Germany). The remaining PCR mix was purified using a QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... the flies were first sated with water and then presented with 100mM sucrose containing 2% blue dye (McCormick) filled in a graduated capillary (VWR, #53432-604). Until the flies became unresponsive to 10 serial food stimuli ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Microbiology 2023Quote: ... 1% agar [Teknova, A7777] containing 40 µg/mL Congo red dye [VWR, AAAB24310-14] and 20 µg/mL Coomassie blue dye [VWR, EM-3300]) in a 10 cm X 10 cm X 1.5 cm plates ...
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bioRxiv - Microbiology 2023Quote: ... The AB food consisted of standard media containing 200 mg/ mL of ampicillin and kanamycin (VWR Life Science; both dissolved in water), 50 mg/ mL of tetracycline (EMD Millipore Corp. ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into single wells (3.5 cm) of the Corning Costar 6-well cell culture plate containing HEK293T/17 cells using the JetPrime Transfection reagent (VWR, Randor, PA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... 400,000 cells were seeded on ethanol-sterilised coverslip in 6 well 3.5 cm diameter plate containing 22 × 22 mm glass coverslips (VWR. Cat# 631-0125). 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™) ...
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bioRxiv - Biochemistry 2023Quote: ... Waltham, MA) containing 0.1% anticlumping agent (Thermo Fischer Scientific, Waltham, MA) in a 125 mL unbaffled shake flask (VWR, Radnor, PA, USA) with a 40 mL working volume ...
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bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were eluted using 0.1 M glycine at pH 3.5 and concentrated using Vivaspin spin filters (VWR) with appropriate MW cut-offs ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cell Biology 2023Quote: ... IRDye 800CW Goat-anti-Rabbit antibody (VWR, Cat No:102673-330) and IRDye 680RD Goat-anti-Mouse antibody (VWR ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated with the secondary antibody and DAPI (1:1000; AppliChem, VWR) for 1 h at RT and mounted with Prolong™ Gold Antifade reagent (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...