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Citations for VWR :
201 - 250 of 255 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... respectively) at 101 °C at atmospheric pressure using a temperature-controlled magnetic hotplate stirrer with a ceramic plate (VWR, Langenfeld, Germany). The PCA concentration was measured using the HPLC analytics protocol described above.The pH was measured with a VWR pH-electrode pHenomenal® 110 (Langenfeld ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Microbiology 2020Quote: ... we used 24.4 ± 1.7 mM fructose (146.4 ± 10.3 mmol C L−1) and the following carboxylates at a concentration of 108.2 ± 8.0 mmol C L−1: Na-acetate (VWR, Solon, OH, USA), Na-butyrate ...
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bioRxiv - Immunology 2021Quote: ... 2mM l-glutamine and 25mM HEPES) were incubated for 96 hours at 37°C either on a 360° rotator (VWR International) or standing stationary (as specified) ...
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bioRxiv - Cancer Biology 2019Quote: ... O/N at 4°C before processing for paraffin embedding and the other half was embedded in Optimun Cutting Temperature compound (VWR chemicals). At least 2 serial sections of 5 μm were stained with ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After “bubbling,” each resulting fluorogenic dye sample was placed in a 37 °C degree water bath (VWR 1228 Digital Water Bath) for fifteen minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lid locks were placed on each tube and incubated at 115°C for 30 min (VWR model 96 place heating block). Samples were dried in a centrifugal evaporator (SpeedVac ...
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bioRxiv - Microbiology 2023Quote: ... all 1123 presumptive Bacillus isolates were grown for 24 hours in TSB at 37°C and 5μl were spotted onto square petri plates (VWR International, USA) with TSA media in a 6 x 6 grid fashion and allowed to dry ...
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bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Tissue for HCR was cryosectioned at 30 μm at -20°C taking coronal sections that were mounted on Superfrost® slides (VWR). The mounted slides were allowed to dry for 24hrs at room temperature before being stored at -80°C before HCR ...
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bioRxiv - Plant Biology 2023Quote: ... Apogee grain were left for 2 days at 5°C in water for imbibition before being placed individually onto cotton in a 24-well tissue culture plate (VWR, USA) and left to germinate for 3 days under high humidity conditions (<90% relative humidity ...
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bioRxiv - Immunology 2023Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then incubated for 10 min at 65 °C and then for 25 min at room temperature with rotation (Tube Rotator, VWR, Radnor, Pennsylvania). Beads were washed with Millipore water ...
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bioRxiv - Cancer Biology 2019Quote: ... in RPMI with 5% FBS and 1% penicillin/streptomycin for 20 minutes in a humidified incubator at 37°C Tissues were washed with PBS and further dissociated in 0.25% Trypsin (VWR, Cat. # VWRL0154-0100) for 30 minutes in the incubator ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Immunology 2020Quote: ... for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL, VWR, Fontenay-sous-Bois, France). Absorbencies were read at 450 nm.
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bioRxiv - Plant Biology 2021Quote: ... Then 200 µL PGM was gently added to the petridish and it was placed in a humid box and incubated at 23°C (incubator, VWR, 89511-416) for 4 or 7 h ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
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bioRxiv - Immunology 2020Quote: ... All tissue was digested at 37°C for 30 min with intermittent vigorous shaking before being passed through a 70 μm strainer (VWR, 734-0003) and neutralized with 8% FCS-DMEM buffer ...
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bioRxiv - Microbiology 2022Quote: Y-strain epimastigotes were cultured in LIT/LDNT medium (37) supplemented with 15% 76°C heat-inactivated fetal bovine serum (FBS) (VWR, USDA certified). To obtain amastigotes ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Physiology 2023Quote: ... we kept the chamber in a 25 −28 °C water bath and then heated the chamber at a rate of 1 °C · min-1 in a water bath (VWR, model: WBE05) containing 2 L of water ...
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bioRxiv - Microbiology 2022Quote: ... albicans isolates were initially cultured by inoculating a single colony in sterile YPD broth at 30°C in an orbital shaker (VWR, model 3500I) at a shaking speed of 200 rpm at a tilted angle of 45° ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min each time, cryopreserved in 30% sucrose (S5-3, Fisher Chemical) in PBS overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (12 μm ...
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bioRxiv - Microbiology 2022Quote: ... Blood was collected by cardiac puncture and then was maintained at 37°C in incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412). Infected red blood cells were enriched by an Accudenz discontinuous gradient as previously described [30] ...
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bioRxiv - Microbiology 2020Quote: ... The liquid and solid fractions of a sample were separated by filtration through a combination of three layers of sterile cheesecloth that had been wrapped separately and baked at 175°C and then transported in sealed 15 ml conical Falcon tubes (catalog number: 62406-200, VWR International, Radnor, PA) on dry ice to the laboratory and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were sliced into 1mm thick coronal slices at -20°C using a metal brain matrix and .22mm razor blades (Ted Pella, 15045; VWR, 55411-050) and were then placed on dry ice and covered to prevent condensation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with 250 nM TG or 10 µM monensin in fresh medium for 0.5 h at 37°C before 40 µM D-biotin (VWR Life Science, Radnor, PA) was added ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was centrifuged (20 min at 4000 rpm) and the supernatant was evaporated (25°C at 210 rpm) on a rotary evaporator (IKA RV10 digital, VWR, Radnor, PA, USA). The dried crude extract was resuspended in 1 ml of acetonitrile for further analyses ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... Cultures were routinely cultivated in growth chambers (LED-41L2, Percival Scientific, Inc.) at 25°C with continuous shaking on an orbital shaker (VWR OS-500 Shaker) at 120 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Microbiology 2020Quote: ... The plates were incubated for four hours at 37 °C under shaking conditions (175 rpm) using a benchtop incubator (VWR® incubator orbital shaker). The 96-well plate was kept inside an airtight plastic container with several wet paper towels to maintain high humidity attached to the shaker ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...