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Citations for VWR :
1 - 50 of 255 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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bioRxiv - Genetics 2020Quote: ... using Phase Lock Gel Heavy tube (713-2536, VWR). The isolated RNAs were reverse transcribed by SuperScript IV with barcode primer (/5Phos/ WWW GTGGA NNNN AGATC GGAAG AGCGT CGTGAT /iSp18/ GGATCC /iSp18/ TACTG AACCG C) ...
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bioRxiv - Genetics 2021Quote: ... transferred to phase-lock heavy tubes (VWR, Cat#: 10847-802), and incubated at room temperature for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... to a 2 ml Phase Lock Gel Heavy tube (713-2536, VWR). The mixture was incubated for 5 min at 30°C at 1100 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Biochemistry 2024Quote: ... transferred to a 5PRIME Phase Lock Gel Heavy spin column (VWR, 733-2478), and spun for 5 min at 20,000 g ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a phase lock gel heavy tube (VWR, 713-2536). The RNA extracted were then phenol/chloroform extracted from the supernatant by adding 200μl of Phenol:Chloroform:Isoamyl Alcohol 25:24:1 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... 5 Prime heavy 2 mL-phase lock gels (#2302830) were from VWR (Radnor, PA). High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then transferred to 5Prime Phase Lock Gel ‘Heavy’ tubes (VWR 733-2478) and centrifuged at 17 G for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The sample was added to a Phase-lock Gel Heavy 2 ml tube (VWR) and centrifuged at 12,000 × g for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and a phenol-chloroform extraction protocol in a 5’-Phase Lock Gel Heavy (VWR, USA). The RNA was precipitated with ethanol and resuspended in nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... after which the mixture was transferred to a Heavy Phase-lock tube (VWR #10847-802) and spun down for 5 minutes at 17,000g ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... The pinching oil phase was a heavy mineral oil supplemented with 1% v/v Span-80 (VWR). Downstream of the segmentation region ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847--802). The aqueous phase was collected ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
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bioRxiv - Physiology 2020Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and a 400W aquarium heater were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and 2×400W aquarium heaters were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Okazaki fragments were enriched by sequential elutions from Source15Q ion exchange columns (VWR 89128-854). Up to 1μg of fragments were ligated to adaptor primer pairs overnight at 16° and cleaned (GeneJET PCR Purification ...
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bioRxiv - Cancer Biology 2021Quote: ... subcutaneous tumor fragments weighing 5–15 mg were homogenized using Kontes tubes with disposable pestles (VWR) in ice-cold 80:20 methanol:water (v/v) ...
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bioRxiv - Microbiology 2020Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or minimal medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Genomics 2022Quote: ... with a traceable digital thermometer (Accuracy: ±0.05°C, resolution: 0.001°, temperature range: –50 to 150°C, VWR, USA), and salinity with a traceable digital portable conductivity meter (Accuracy ...
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bioRxiv - Microbiology 2023Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or defined medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Cancer Biology 2020Quote: ... at 56°C (VWR Seradigm, Lot 120B14). All cells were cultured in a Heracell (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... + 0.5% BSA-c (Aurion, VWR, Cat# 25557) + 2% fish skin gelatin (Sigma ...
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bioRxiv - Pathology 2019Quote: ... The T1 fragment was placed into a VWR Personal Low Temperature Incubator (VWR Catalog number 89511-416) with varying temperature profiles depending on condition ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 100 °C on block heater (VWR, Radnor, PA). 25ug of protein was loaded into homemade 4-8% gradient polyacrylamide gels ...
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bioRxiv - Microbiology 2022Quote: ... 1 volume of cold (4°C) TCA (VWR) was mixed with 4 volumes of supernatant and incubated on ice for 60 minutes ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counted using C-chip disposable hemocytometer (VWR, International Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Buffer C consisted of 0.1% phosphoric acid (85%, VWR) in 60% acetonitrile (acidic wash ...
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bioRxiv - Cell Biology 2023Quote: ... and a 2x volume −20°C ethanol (VWR Chemicals). After overnight incubation at −20°C the DNA was pelleted at 17000g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 4 mL of 80% Methanol (-20°C, VWR, #85800290) was directly added to cells with the flasks on ice ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Neuroscience 2022Quote: ... with chamber temperature of 21 ± 3°C and object temperature of 19±3°C and sections were mounted directly on Superfrost glass (631-0108, VWR, Leuven, The Netherlands) in series of 10 per each brain and stored at −80°C.