Labshake search
Citations for VWR :
151 - 200 of 259 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... 3.5 mL of 18.2 MΩ deionised water (Elga PURELAB flex 3 system, VWR International, Edmonton, AB, Canada) was then added to effect a separation into a polar methanol/water layer and a chloroform layer ...
-
bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
-
bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
-
bioRxiv - Neuroscience 2019Quote: ... sections were washed in PBS (3×10 min) and mounted on Superfrost Plus slides (VWR, Radnor, PA) using Fluoromount G (Southern Biotechnology Associates ...
-
bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
-
bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
-
bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
-
bioRxiv - Neuroscience 2022Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
-
bioRxiv - Microbiology 2024Quote: ... Moore swabs were constructed by folding 3 by 24-inch strips of cheese cloth (VWR, Radnor, PA) 8 times to form an 8-ply square pad ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
-
bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
-
bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
-
bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
-
bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
-
bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
-
bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tumor growth was monitored weekly and tumor volume was measured along 3 axes with calipers (VWR 62379-531). Tumors and lungs were harvested at time of tumor burden (total tumor volume of 2cm3 ...
-
Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
-
bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.4% Zinc) and resuspended in 5 mM HCl at 3 mg/ml (Cat# 20252.244, VWR Chemicals, Dresden, Germany). The purity of insulin was confirmed by mass spectrometry under denaturing conditions where only monomeric and dimeric insulin with a mass of 5803.651 +/-0.003 Da was detectable ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at room temperature on a shaker in presence of 3 μg LysC Endopeptidase (VWR). Next ...
-
bioRxiv - Genomics 2020Quote: ... were co-transfected with the respective lentiviral expression vector (∼1:1:3 molar ratio) using Trans-IT (VWR) and OptiMEM (Gibco) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3 μm-thick sections were obtained and counterstained with Liquid-Stable Nuclear Fast Red (VWR Life Science AMRESCO).
-
bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
-
bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
-
bioRxiv - Genetics 2023Quote: The muscarinic receptor antagonist atropine ((RS)-(8-Methyl-8-azabicyclo[3.2.1]oct-3-yl, VWR Cat. TCA0754-005G) was dissolved in ethanol and added to 1M (HS ...
-
bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
-
bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer; VWR, Radnor, PA, United States). The supernatant was transferred to a clean tube ...
-
bioRxiv - Microbiology 2024Quote: ... were added by incubating samples for 3.5 hours at 37°C in 3% BSA (VWR Cat# 97061-422) in PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 1 hour in 2 ml of 3% BSA in PBS (VWR, cat#: 97063-624). Cells were then washed three times with 2 ml of PBS for 5 minutes each and mounted with Prolong Diamond Antifade Mountant (Molecular Probes ...
-
bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
-
bioRxiv - Microbiology 2020Quote: ... were cut with a cryostat and 3 sections of 20 µm were placed on each microscope glass slide (VWR). Sections were fixed with acetone for 10 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... 100 µl of the cell suspension were injected using 25 G needles (BD Microlance TM 3. VWR 613-4952). Cell numbers and viability (required to be above 90% ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 3-D spheroids were generated by seeding 2.0E6 cells per well of a 6-well cell repellent plate (VWR) in a final volume of 4.0 mL complete media and placed on an orbital shaker at 95 RPM for 24 hours ...
-
bioRxiv - Synthetic Biology 2020Quote: 3 biological replicates of each isolate were grown overnight in 96-deep-well plates (VWR International, cat #10755-248) in LB media ...
-
bioRxiv - Neuroscience 2019Quote: ... Novaplus) followed by freshly prepared 2% glutaraldehyde/2% paraformaldehyde in 0.1M PB solution filtered with #3 filter paper (VWR) and pH to 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: Custom Eppendorf tubes for extract recovery were created by cutting approximately 3 mm from the bottom of a standard 0.5 mL Eppendorf tube (VWR). The tubes were then soaked in 70% ethanol to remove small molecule contaminants ...
-
bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
-
bioRxiv - Microbiology 2022Quote: ... was diluted 1:3 in cold PBS and 100 μL was added to 0.4 μm polycarbonate trans-well plate inserts (VWR). The Transwell plates were placed 4°C for 30 min to let the Matrigel settle and were then moved to an anaerobic chamber to gel at 37°C for 24 h ...
-
bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...
-
bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue was washed 3 times in PBS before blocking for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
-
bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).
-
bioRxiv - Microbiology 2024Quote: ... Plates were then washed 5 times in PBS before addition of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) substrate (VWR), and incubation at 37°C for 15 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...