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Citations for VWR :
1 - 50 of 259 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Immunology 2020Quote: ... and lectin resin (VWR) for gB Domain I ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Microbiology 2020Quote: ... The soluble fractions were transferred to cuvettes (VWR), and absorbance of the samples at 420 nm was recorded using a Bio-Rad Spectrophotometer ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
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bioRxiv - Immunology 2023Quote: ... 3% SDS (151-21-3, VWR Life Science) and 100 mM Tris–HCl [pH 6.8](1.00317 ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Cell Biology 2020Quote: ... 3% glucose (VWR), 3.5% active dried yeast (SLS) ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (VWR), 0.1% NP-40 substitute (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubating in 5% normal horse serum for 60 min to block nonspecific binding (VWR, 102643-676). Afterwards ...
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bioRxiv - Cancer Biology 2019Quote: ... G418 (VWR, 970-3-058), for several passages ...
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bioRxiv - Neuroscience 2022Quote: ... 3% Acetonitrile (VWR Cat#83640.320), 1 μg of LysC (Wako Cat#129-02541) ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% hydrogen peroxide (VWR) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and 3 % of BSA (VWR) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
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bioRxiv - Cell Biology 2023Quote: ... covered with a glass plate (3" x 3" glass plate from VWR Vertical Gel Electrophoresis Systems), exposed to 7.5 minutes of glass plate filtered (UVA only ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM MnCl2 (VWR; Cat. # 1.05927.1000), 3 µM Na- orthovanadate (Sigma ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 (VWR, 97062-848) supplemented with 0.1% SUPERase•In™ RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 3% Bovine Serum Albumin (VWR, #97061-416) in PBS ...
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bioRxiv - Systems Biology 2024Quote: ... in 3% BSA (VWR Cat# 97061-422) in PBS for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... on 3% agarose gel (VWR, Radnor, PA) with RedSafe Nucleic Acid Staining Solution (Bulldog Bio ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Molecular Biology 2021Quote: ... and washed/fixed with 3:1 methanol (VWR)/glacial-acetic acid (Carl Roth ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 15min washes in 5x SSCT (DAPI, VWR International ...
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bioRxiv - Microbiology 2020Quote: ... to 3 parts 0.5 M ammonia (VWR #BDH153312K) for 14 h ...
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bioRxiv - Physiology 2023Quote: ... Peroxidases were blocked by 3% hydrogen peroxide (VWR) in methanol (National Diagnostics ...
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bioRxiv - Developmental Biology 2023Quote: ... After fixing with 3:1 methanol (VWR 20846.326): glacial acetic acid (VWR 20102.292) ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...