Labshake search
Citations for VWR :
201 - 250 of 282 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
-
bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
-
bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
-
bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
-
bioRxiv - Biophysics 2019Quote: ... The reactions were quenched by the addition of SDS-PAGE loading buffer and analyzed by 10% Tricine-SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (VWR).
-
bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone, 0.5% yeast extract, 1% NaCl, 100 g/L ampicillin (VWR # V0339), 0.4% SeaPrep agarose (VWR ...
-
bioRxiv - Biophysics 2022Quote: ... each flowcell consists of a set of five parallel microchannels that are sandwiched between a quartz microscope slide (G. Finkenbeiner) and a borosilicate glass coverslip (VWR). Prior to assembling the flowcell ...
-
bioRxiv - Microbiology 2023Quote: ... were generated by centrifugation of the grown culture at 5800 x g for 20 min and subsequent filter-sterilization (0.2 µm, VWR™). SM samples were prepared freshly before each experiment under anaerobic conditions ...
-
bioRxiv - Neuroscience 2023Quote: ... and the sections were finally mounted under glass coverslips with Fluoromount-G (Cat No. 100241-874, VWR, Radnor, PA, USA).
-
bioRxiv - Synthetic Biology 2023Quote: ... We grew the transformed library in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 7 cm diameter column loaded with 20 g of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the mobile phase ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Microbiology 2024Quote: ... smegmatis WT and ΔmftG strains were grown in 320 mL HdB-Tyl medium supplemented with 1 g L−1 ethanol as the sole carbon source in 2 L flasks covered by breathable rayon film (VWR) in triplicates and incubated at 37 °C and 210 rpm with a starting OD600 of 0.1 for WT and 2.0 for ΔmftG for a period of 48 h.
-
bioRxiv - Genomics 2024Quote: ... supplemented with PIM(ABS) (Prodo Labs) and PIM(G) (Prodo Labs) and incubated in a T-150 non-tissue culture treated flask (VWR) for recovery at 37°C and 5% CO2 overnight ...
-
bioRxiv - Genomics 2019Quote: ... then centrifuged at 4000 x g for ten minutes and filtered the supernatant through a Poly-ethersulfone filter (VWR, 28145-505) to remove cellular debris ...
-
bioRxiv - Genetics 2020Quote: ... the cells were resuspended in 1 ml of sterile water and spread on a plate containing minimal media lacking the corresponding amino acid or nucleotide (YNB or Synthetic Complete (SC): 6.7 g/l yeast nitrogen base (VWR 97064-162), 20 g/l glucose ...
-
bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 14000×g for 40 min and a Macrosep advanced filter (3000 Da MW cutoff, 20 ml; catalogue no. 89131-974; VWR, USA) was used to remove the free biotin ...
-
bioRxiv - Biochemistry 2023Quote: ... Each sample (10 μL) was loaded and separated on an SDS-PAGE followed by Coomassie G-250 staining (VWR, Cat. # 0615) to visualize the overexpression of the desired fusion protein.
-
bioRxiv - Molecular Biology 2023Quote: ... the suspension centrifuged for five minutes at 300 x g again and the resulting pellet flash frozen in dry ice and Methanol (VWR, 20847.307). Pellets were thawed on ice ...
-
bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
-
bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
-
bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
-
bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
-
bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
-
bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Each bone was then cut in half and transferred to a 1.5 ml microcentrifuge tube containing a G-Tube microcentrifuge tube (VWR, Radnor, PA, USA) that had been punctured at the bottom with a 15 gauge needle ...
-
bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
-
bioRxiv - Genetics 2020Quote: ... Transformants were pooled and cultured in low-pH Sabouraud Dextrose Casamino Acid media (SDCAA, per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
-
bioRxiv - Biochemistry 2022Quote: ... Individual yeast colonies were picked into 4mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
-
bioRxiv - Microbiology 2022Quote: ... Mycobacterium smegmatis (ATCC 700084) was maintained in 7H9 media supplemented with 10% ADS enrichment: 50 g bovine serum albumin (VWR, Radnor, PA), 20 g dextrose (Fisher Scientific) ...
-
bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
-
bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
-
bioRxiv - Microbiology 2023Quote: ... We prepared a 1:10 dilution with approximately 1 g of fecal sample that was measured with a sterile plastic spoon (VWR International, Dietikon, Switzerland) and subsequently suspended in 9 mL of anaerobic dilution solution ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins in the pellet were solubilized by sonication (incubation in Ultrasonic Cleaner bath, VWR) for 10 min followed by incubation for 60 min at 4 °C under rotation ...
-
bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
-
bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
-
bioRxiv - Biochemistry 2024Quote: The protein was further purified by anion exchange chromatography (HiTrap Q, VWR 17-1154-01) with a linear gradient of increasing NaCl salt concentration ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...