Labshake search
Citations for VWR :
151 - 200 of 282 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... with L-glutamine and 4.5 g/L glucose supplemented with 10% Fetal Bovine Serum (FBS; VWR) and 1% penicillin/streptomycin (Corning) ...
-
bioRxiv - Microbiology 2023Quote: ... in tryptic soy broth (TSB, Difco) or in M9 minimal medium (KH2PO4 3.4 g/l, VWR; K2HPO4 2.9 g/l ...
-
bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... The M63 minimal media was prepared by diluting 15.6 g of M63 broth powder (VWR Life Science) in 0.9 L ultrapure water ...
-
bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
-
bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
-
CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
-
bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
-
bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
-
bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer: 2.39 g/L HEPES (VWR 3638C017), 80.7 g/L sucrose (Sigma-Aldrich S0389) ...
-
bioRxiv - Cell Biology 2022Quote: ... and 0.37 g Ethylenediaminetetraacetic acid (EDTA) per liter) (all reagents were from VWR Life Science Seradigm, Randor, PA), and the 6X sample buffer was 0.01% Orange G (Fisher Scientific ...
-
bioRxiv - Microbiology 2019Quote: The phosphate buffer solution for NMR was prepared by dissolving 102 g of anhydrous KH2PO4 (VWR, Darmstadt, Germany), 0.5 g of TSP (3(trimethylsilyl ...
-
bioRxiv - Pathology 2021Quote: ... The plate was centrifuged at 300 g for 10 min and fixed by directly adding paraformaldehyde (PFA, VWR) to the supernatant to a final concentration of 4% ...
-
bioRxiv - Microbiology 2019Quote: ... Tissue cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, (VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
-
bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
-
bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
-
bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
-
bioRxiv - Microbiology 2023Quote: ... centrifuged for 15 min at 14,000 × g through 10 kDa molecular weight cut-off filters (modified PES, VWR), and transferred into HPLC vials.
-
bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
-
bioRxiv - Developmental Biology 2023Quote: ... mice were weighed 1 hr prior to harvest and injected with 0.04 µmol/g puromycin (VWR, #75844-852).
-
bioRxiv - Microbiology 2024Quote: ... The samples were centrifuged at 17,000 × g for 20 min and supernatants were sterilized using 0.2 µm cellulose acetate membrane filters (VWR) and kept at −20 °C until further analysis ...
-
bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
-
bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
-
bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
-
bioRxiv - Immunology 2023Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a 0.45 µm nitrocellulose membrane (VWR), by electroblot ...
-
bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
-
bioRxiv - Cancer Biology 2019Quote: ... 100 µl of the cell suspension were injected using 25 G needles (BD Microlance TM 3. VWR 613-4952). Cell numbers and viability (required to be above 90% ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
-
bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.
-
bioRxiv - Microbiology 2021Quote: ... we added 40 mg of powder yeast extract g−1 dry soil as a C and nutrient source (VWR). We chose yeast extract over glucose to provide more varied forms of C and thus stimulate more diverse microorganisms (Fierer et al. ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Soil (10 g) was suspended in deionized water (50 mL) and mixed on an orbital shaker (OS-500, VWR) for 2 hours ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
-
bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
-
bioRxiv - Microbiology 2023Quote: ... Sorbarod filter samples were homogenised via placement in half-strength thioglycolate medium (14.5 g/L; Oxoid, Basingstoke, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR, Lutterworth, UK). Samples were vortexed (30 seconds) ...
-
bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
-
bioRxiv - Microbiology 2023Quote: ... 8% CO2 with continuous shaking and culture supernatant was then clarified by centrifugation at 4000 x g for 20 minutes and filtered using 0.2 μm vacuum filtering system (VWR). SOSIP glycoproteins were purified from the supernatant by affinity chromatography and further by size exclusion chromatography as follows ...
-
bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
-
bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
-
bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
-
bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...