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Citations for VWR :
301 - 340 of 340 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Blood was collected by cardiac puncture and then was maintained at 37°C in incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412). Infected red blood cells were enriched by an Accudenz discontinuous gradient as previously described [30] ...
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bioRxiv - Microbiology 2020Quote: ... The liquid and solid fractions of a sample were separated by filtration through a combination of three layers of sterile cheesecloth that had been wrapped separately and baked at 175°C and then transported in sealed 15 ml conical Falcon tubes (catalog number: 62406-200, VWR International, Radnor, PA) on dry ice to the laboratory and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were sliced into 1mm thick coronal slices at -20°C using a metal brain matrix and .22mm razor blades (Ted Pella, 15045; VWR, 55411-050) and were then placed on dry ice and covered to prevent condensation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with 250 nM TG or 10 µM monensin in fresh medium for 0.5 h at 37°C before 40 µM D-biotin (VWR Life Science, Radnor, PA) was added ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was centrifuged (20 min at 4000 rpm) and the supernatant was evaporated (25°C at 210 rpm) on a rotary evaporator (IKA RV10 digital, VWR, Radnor, PA, USA). The dried crude extract was resuspended in 1 ml of acetonitrile for further analyses ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... Cultures were routinely cultivated in growth chambers (LED-41L2, Percival Scientific, Inc.) at 25°C with continuous shaking on an orbital shaker (VWR OS-500 Shaker) at 120 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Microbiology 2020Quote: ... The plates were incubated for four hours at 37 °C under shaking conditions (175 rpm) using a benchtop incubator (VWR® incubator orbital shaker). The 96-well plate was kept inside an airtight plastic container with several wet paper towels to maintain high humidity attached to the shaker ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
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bioRxiv - Neuroscience 2022Quote: ... Microscope slides with serially cut nerves were rinsed in 1x PBS for 5 min and air dried by incubation at 60°C for 45 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −20 °C in an anti-freeze solution (30 % glycerol [Merck Millipore, Germany], 30 % ethylene glycol [VWR International, USA] and 10 % TBS) until further processing ...
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bioRxiv - Neuroscience 2024Quote: ... Microscope slides with serially sectioned nerves were rinsed in 1× PBS for 5 min (repeated twice) and air-dried by incubation at 70°C for 10 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Neuroscience 2023Quote: ... for cryoprotection and allowed to sink at 4°C prior to being cryosectioned at 20 μm and placed on charged slides (VWR Superfrost® Plus, 48311-703).
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Physiology 2022Quote: ... frozen and crushed in liquid nitrogen for protein and RNA extraction or ii) embedded in Tissue-Tek OCT compound (VWR, Mississauga, Canada) and frozen in isopentane cooled with liquid nitrogen for cryostat sectioning54 or iii ...
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bioRxiv - Cell Biology 2022Quote: ... Samples containing 20-50 µg protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose or polyvinylidene fluoride membrane (10600023, VWR, Radnor, PA). After blocking the membrane for 1h in 3% BSA in Tris- buffered saline with Tween 20 (TBS-T) ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...