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Citations for VWR :
1 - 50 of 340 citations for Borrelia burgdorferi C t Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Wells of the 12-well microtiter plate (VWR 734-2324 T C-treated 12-well plates) were prepared just before inoculation and imaging by coating the bottom of each well with 15 µl of Con A and allowing the wells to dry ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Pathology 2022Quote: ... 0.02% Tween20 (VWR, hereafter PBS-T), permeabilized (2 x 10min. ...
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bioRxiv - Immunology 2023Quote: ... in PBS-T (PBS (VWR 45000-436), 0.5% bovine albumin serum (Sigma Aldrich A7979) ...
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bioRxiv - Bioengineering 2023Quote: ... Vented caps from standard T-25 flasks (VWR) were allowed to rest on top of the inlet and outlet of the Biodome – maintaining an enclosed environment and CO2 exchange with the media ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Physiology 2020Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and a 400W aquarium heater were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Magnetic Drive Centrifugal Pump (300W/600W/950W @ 0°C/10°C/20°C; VWR) and 2×400W aquarium heaters were used to maintain the temperatures in the cold and warm bath ...
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bioRxiv - Neuroscience 2022Quote: ... Following washes in PBS-T (VWR, Cat. #76371-736), membranes were incubated with IRDye® 680RD Goat-anti-Mouse (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... then sonicated 16 minutes (VWR Ultrasonic Cleaner USC-T) to obtain 30nm extruded Small Unilamellar Vesicles (SUVs) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown in T-75 flasks (flow cytometric analysis) or T-175 flasks (Fluorescence Activated Cell Sorting) (VWR International; Bridgeport, NJ, USA) every 4-5 days until confluency ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hrs in T cell media containing RPMI 1640 (VWR), 5% heat inactivated FBS ...
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bioRxiv - Genomics 2023Quote: ... the infected cells were passaged into T-225 flasks (VWR International LLC #47743-882) containing media supplemented with 2 μg/mL puromycin ...
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bioRxiv - Immunology 2022Quote: ... T cells from tumors were isolated with a 40/70 percoll gradient (VWR 17-0891). When sorted for RNA sequencing ...
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bioRxiv - Immunology 2023Quote: ... All T cell cultures were maintained in RPMI medium containing 10% FBS (VWR, 97068–085) and 30 IU/mL human interleukin-2 (hIL-2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Microbiology 2020Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or minimal medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Genomics 2022Quote: ... with a traceable digital thermometer (Accuracy: ±0.05°C, resolution: 0.001°, temperature range: –50 to 150°C, VWR, USA), and salinity with a traceable digital portable conductivity meter (Accuracy ...
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bioRxiv - Microbiology 2023Quote: ... monocytogenes strains were grown at 37°C or 30°C in brain heart infusion (BHI) medium (237500; VWR) or defined medium supplemented with glucose as the sole carbon source ...
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bioRxiv - Cancer Biology 2020Quote: ... at 56°C (VWR Seradigm, Lot 120B14). All cells were cultured in a Heracell (Thermofisher ...
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bioRxiv - Cell Biology 2023Quote: ... + 0.5% BSA-c (Aurion, VWR, Cat# 25557) + 2% fish skin gelatin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.6 × 10^6 cells per temperature were seeded in T-25 cell culture flasks (VWR) in an appropriate volume of culture medium ...
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bioRxiv - Systems Biology 2021Quote: BJ-5ta cells were lifted from T-150 culture flasks using 0.25% trypsin (VWR 45000-664) for 5–10 min ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 1:5000 dilution in PBS-T) for one hour and developed with chemiluminescent reaction (VWR, 490005-008).
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Cancer Biology 2024Quote: ... at a cell density of 4.166×105 cells/mL in tissue culture-treated T-75 flasks (VWR) (7) ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Neuroscience 2019Quote: ... 100 °C on block heater (VWR, Radnor, PA). 25ug of protein was loaded into homemade 4-8% gradient polyacrylamide gels ...
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bioRxiv - Microbiology 2022Quote: ... 1 volume of cold (4°C) TCA (VWR) was mixed with 4 volumes of supernatant and incubated on ice for 60 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were counted using C-chip disposable hemocytometer (VWR, International Ltd ...
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bioRxiv - Cell Biology 2022Quote: ... Buffer C consisted of 0.1% phosphoric acid (85%, VWR) in 60% acetonitrile (acidic wash ...
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bioRxiv - Cell Biology 2023Quote: ... and a 2x volume −20°C ethanol (VWR Chemicals). After overnight incubation at −20°C the DNA was pelleted at 17000g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 4 mL of 80% Methanol (-20°C, VWR, #85800290) was directly added to cells with the flasks on ice ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were subsequently washed 4x with PBS-T and developed with 2,2-azinobis(3ethylbenzthiazolinesulfonic acid) (ABTS) (VWR 95059-146) substrate for 5 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... In the final steps the beads were resuspended in 20 μL 0.1 % formic acid in water and sonicated in an Ultrasonic Cleaner USC-T (VWR) for 10 min and the supernatant was transferred to a new plate ...
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Neuroscience 2022Quote: ... with chamber temperature of 21 ± 3°C and object temperature of 19±3°C and sections were mounted directly on Superfrost glass (631-0108, VWR, Leuven, The Netherlands) in series of 10 per each brain and stored at −80°C.
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... Brains were washed three times in PBS-T and mounted in Mowiol mounting medium on a borosilicate glass side (number 1.5; VWR International). Primary antibodies used were ...
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bioRxiv - Biochemistry 2020Quote: For maintenance C2C12 myoblasts (kindly provided by DM Rossi, ICS Maugeri, Pavia) were grown in 75 cm2 T-flasks (VWR) at 37 °C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1×105 T cells or Jurkat T cells were co-cultured with target cells at a 1:1 ratio in 96 well round bottom plates (VWR). To exogenously activate T-cells ...