Labshake search
Citations for VWR :
401 - 450 of 469 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Two-µl spots of each dilution were plated on selective (20 µg/mL thiamphenicol) and unselective square (90×15 mm, VWR international) BHI plates with or without 200 ng/mL anhydrotetracycline (ATc) ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... animals were randomly assigned to individual cages under one of four groups (n=8 per group): Wild-type (WT) C57BL/6 mice with access to a free running wheel (VWR) or a locked wheel (SED) ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were homogenized manually using a Pellet Pestle Motor (Kimble, 749540) and an RNase-Free pellet pestle (VWR, KT749510-0590) for 30 seconds following the addition of 140 μL of TRIzol to a total of 240 μL ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... cecal contents from specific-pathogen free (SPF) mice were resuspended in 10% weight/volume (w/v) Bacto Brain Heart Infusion (BHI) (VWR) supplemented with 0.05% w/v L-cysteine (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Bioengineering 2023Quote: ... and the neutrophils and NETs were collected by repeated rinsing of the flask in cold DNAse and RNAse free ultrapure water (VWR) before centrifugation at a speed of 450 xg for 10 min to remove neutrophils ...
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bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
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bioRxiv - Developmental Biology 2024Quote: ... Blots were washed with 1x TBS with 0.1% Tween-20 (TBST) and blocked using 5% IgG-free BSA (VWR RLBSA50). Primary antibodies (Supplemental Table 2 ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Genomics 2024Quote: ... supplemented with PIM(ABS) (Prodo Labs) and PIM(G) (Prodo Labs) and incubated in a T-150 non-tissue culture treated flask (VWR) for recovery at 37°C and 5% CO2 overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... We grew the transformed library in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 7 cm diameter column loaded with 20 g of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the mobile phase ...
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bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone, 0.5% yeast extract, 1% NaCl, 100 g/L ampicillin (VWR # V0339), 0.4% SeaPrep agarose (VWR ...
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bioRxiv - Biophysics 2022Quote: ... each flowcell consists of a set of five parallel microchannels that are sandwiched between a quartz microscope slide (G. Finkenbeiner) and a borosilicate glass coverslip (VWR). Prior to assembling the flowcell ...
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bioRxiv - Microbiology 2023Quote: ... were generated by centrifugation of the grown culture at 5800 x g for 20 min and subsequent filter-sterilization (0.2 µm, VWR™). SM samples were prepared freshly before each experiment under anaerobic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... and the sections were finally mounted under glass coverslips with Fluoromount-G (Cat No. 100241-874, VWR, Radnor, PA, USA).
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bioRxiv - Microbiology 2024Quote: ... Cultures were pelleted at 3200 x g for 10 minutes at room temperature then washed in 1X volume blood bank saline (89370-096, VWR) and resuspended in 7H9 + 0.5% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... then pelleted at 3200 x g for 10 minutes at 4°C and washed twice with pre-chilled blood bank saline (89370-096, VWR). Pellets were resuspended in 0.8 mL 3:1:0.004 acetonitrile:methanol:formic acid + 10 nM verapamil (V105 ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Biochemistry 2024Quote: ... 200-300 million K-562 cells were collected by centrifugation (5 min at 200 x g, Mega Star 600 R centrifuge (VWR), rotor #75005701 (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Dye-loaded nanocarriers were purified from free dye by dialysis or 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were diluted with ultrapure water to a final concentration of 0.475 N nitric acid and transferred to metal-free centrifuge vials (VWR, 89049-172) for subsequent ICP-MS analyses.
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bioRxiv - Immunology 2024Quote: ... was used for these studies.[41] hTCEpi cells were maintained in serum-free keratinocyte growth medium containing 0.06 mM calcium and 6 mM glucose (VWR, Radnor, PA). Calcium levels were increased to 0.15 mM calcium and media was further supplemented with bovine pituitary extract (0.0004 mL/mL) ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in 1 ml of sterile water and spread on a plate containing minimal media lacking the corresponding amino acid or nucleotide (YNB or Synthetic Complete (SC): 6.7 g/l yeast nitrogen base (VWR 97064-162), 20 g/l glucose ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample (10 μL) was loaded and separated on an SDS-PAGE followed by Coomassie G-250 staining (VWR, Cat. # 0615) to visualize the overexpression of the desired fusion protein.
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bioRxiv - Molecular Biology 2023Quote: ... the suspension centrifuged for five minutes at 300 x g again and the resulting pellet flash frozen in dry ice and Methanol (VWR, 20847.307). Pellets were thawed on ice ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 14000×g for 40 min and a Macrosep advanced filter (3000 Da MW cutoff, 20 ml; catalogue no. 89131-974; VWR, USA) was used to remove the free biotin ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Microbiology 2024Quote: ... Phages were routinely titered by the soft agar overlay method(28) using TSA bottom plates and top agar consisting of 10 g/L Bacto™ tryptone (VWR), 10 g/L NaCl and 0.5% Bacto™ agar (VWR) ...
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bioRxiv - Microbiology 2024Quote: ... aureus strains were generated as previously described 101 and transformed with ∼5 µg of the pIMAY construct using the BioRad Gene Pulser Xcell electroporation system and 1 mm disposable electroporation cuvettes (VWR Signature). The pulse was 21kV/cm ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were stimulated with p25 peptide-pulsed (sequence: FQDAYNAAGGHNAVF from Genescript) iBMDMs or FLAMs that were irradiated with mitomycin (25 µg/mL) (VWR, Cat no. TCM2320) and had been pre-treated with DMSO ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 1x105 neurons/ml so that 1x105 neurons were seeded onto poly-D-lysine (10 µg/mL) coated acid-etched coverslips (VWR,, cat#MSPP-P06G1520F) and cultured at 37°C plus 5% CO2 ...
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bioRxiv - Synthetic Biology 2024Quote: Transformed colonies were scraped from the agar plate of the sample group into 10 mL of LB medium supplemented with 50 µg/mL kanamycin (VWR, Cat# 0408-10G) in a sterile 50 mL polypropylene conical tube (Corning ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500). An appropriate volume of reaction mastermix was prepared with the following composition ...
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bioRxiv - Microbiology 2024Quote: ... cell line developed and characterized by our laboratory was used in this study.(31) Cells were maintained in defined serum-free keratinocyte growth medium (KGM) containing growth factor supplements (KGM, VWR, Randor, PA) and a 10% solution consisting of penicillin ...
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bioRxiv - Microbiology 2024Quote: ... was used in this study.23 Cells were cultured in serum-free keratinocyte growth medium with growth factor supplements (KGM, VWR, Randor, PA) and 10% penicillin ...