Labshake search
Citations for VWR :
351 - 400 of 469 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...
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bioRxiv - Microbiology 2024Quote: ... Four sets of dilutions (100 to 10-3) of the filtered lysates were created in standard 96 well plates (VWR, cat. no. 25381-056), then arrayed in quadrants of a single 384-well plate ...
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bioRxiv - Bioengineering 2020Quote: ... The M63 minimal media was prepared by diluting 15.6 g of M63 broth powder (VWR Life Science) in 0.9 L ultrapure water ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Microbiology 2021Quote: ... difficile strains containing the phiLOV2.1 reporter plasmid were grown anaerobically at 37°C in BHIS-Tm10 for 12 - 16 h and inoculated 1:50 into fresh BHIS-Tm10 containing indicated treatments (1.70 µg mL−1 vancomycin (VWR), 0.67 µg mL−1 fidaxomicin (Cayman Chemical) ...
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bioRxiv - Bioengineering 2022Quote: ... tissue culture 96-well plates (Coastar) were pre-treated with 100 µL of streptavidin at 10 µg/mL (VWR) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer: 2.39 g/L HEPES (VWR 3638C017), 80.7 g/L sucrose (Sigma-Aldrich S0389) ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.37 g Ethylenediaminetetraacetic acid (EDTA) per liter) (all reagents were from VWR Life Science Seradigm, Randor, PA), and the 6X sample buffer was 0.01% Orange G (Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... The plate was centrifuged at 300 g for 10 min and fixed by directly adding paraformaldehyde (PFA, VWR) to the supernatant to a final concentration of 4% ...
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bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged for 15 min at 14,000 × g through 10 kDa molecular weight cut-off filters (modified PES, VWR), and transferred into HPLC vials.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Developmental Biology 2023Quote: ... mice were weighed 1 hr prior to harvest and injected with 0.04 µmol/g puromycin (VWR, #75844-852).
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Microbiology 2024Quote: ... bile was obtained from the gallbladder with a 31 G short-needle insulin syringe (Sol-M®, VWR) and aspirated into 90 μl sterile PBS ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with PBST and then incubated with 4′,6-diamidino-2-phenylindole (DAPI, 5 µg/mL in PBS) (VWR) and phalloidin (1.5 µM in DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... either D-luciferin (Fisher #LUCK-2G; 150 µg / mL read concentration) or Rluc substrate (VWR # PAP1232; 17 µM read concentration). Maximum cytotoxicity was defined as luminescent signal from wells containing only media while no cytotoxicity was defined by wells containing only target cells ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL were spotted onto 60 mL of colony morphology medium (1% tryptone, 1% agar [Teknova, A7777] containing 40 µg/mL Congo red dye [VWR, AAAB24310-14] and 20 µg/mL Coomassie blue dye [VWR ...
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bioRxiv - Bioengineering 2023Quote: ... Plate colonies were picked at random and used to inoculate 10mL LB media with 100 µg/mL carbenicillin in 15mL culture tubes (VWR). Cultures were once again grown overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 50mM Tris-HCl pH 8.1,5mM EDTA) containing protease inhibitors (Pierce mini tablets EDTA-free, VWR, Radnor, PA, Cat #PIA32955) to obtain nuclear extracts ...
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bioRxiv - Bioengineering 2021Quote: ... CQ loaded nanocarriers were purified from free CQ by 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution.
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Microbiology 2021Quote: ... Free biotin was removed by Macrosep Advance Spin Filter 3K MWCO 20 mL (Cat no. 89131-974, VWR, USA). Aliquots of the samples were stored at −80°C for use as input in western blot ...
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bioRxiv - Bioengineering 2023Quote: ... the serum-free CM was filtered with Pore Size 0.22 µm Vacuum Filtration Systems (10040-460; VWR, PA, USA) to remove undesired large debris ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5mL warm serum free media (SFM) was added on top of the Corning GFR Matrigel inserts (VWR, Radnor, PA). Matrigel was allowed to rehydrate for 2 h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... using a digital scale (TC-204, Denver instruments, Arvada, CO, USA) and then moved into a forced-air oven (VWR Oven F Air 104 L, VWR, Radnor, PA) at 65 °C to dehydrate ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.
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bioRxiv - Microbiology 2021Quote: ... we added 40 mg of powder yeast extract g−1 dry soil as a C and nutrient source (VWR). We chose yeast extract over glucose to provide more varied forms of C and thus stimulate more diverse microorganisms (Fierer et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... Soil (10 g) was suspended in deionized water (50 mL) and mixed on an orbital shaker (OS-500, VWR) for 2 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Microbiology 2023Quote: ... Sorbarod filter samples were homogenised via placement in half-strength thioglycolate medium (14.5 g/L; Oxoid, Basingstoke, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR, Lutterworth, UK). Samples were vortexed (30 seconds) ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2023Quote: ... 8% CO2 with continuous shaking and culture supernatant was then clarified by centrifugation at 4000 x g for 20 minutes and filtered using 0.2 μm vacuum filtering system (VWR). SOSIP glycoproteins were purified from the supernatant by affinity chromatography and further by size exclusion chromatography as follows ...
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bioRxiv - Microbiology 2024Quote: ... at 20,000 × g for 1 h at 4 °C and resuspended in 10% (v/v) of the start volume with cold TN50 buffer (1.21 g/L Tris-HCl (VWR), 2.92 g/L NaCl (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: Bone marrow from femurs of 6-to 8-week-old female C57BL/6 mice were flushed with Roswell Park Memorial Institute (RPMI) 1640 media with 1X L-glutamine supplemented with 5.95 g/L HEPES (VWR), and 1 mM sodium pyruvate to retrieve all cells ...
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bioRxiv - Microbiology 2024Quote: ... Isolates were stored at - 80°C in freezing medium (-Nutrient broth (Oxoid CM0067) 21,25 g/L (2,13%)-Glycerol 85% (VWR 24384) 184,5 g/L (=150 mL/L ...
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bioRxiv - Molecular Biology 2024Quote: Purified chromosomes 3 or 19 were cytocentrifuged (Cytospin3, Shandon) at 163 g for 10 min onto Polysine slides (VWR). After mounting with Vectashield containing DAPI (Vector Laboratories) ...
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bioRxiv - Bioengineering 2024Quote: ... approximately 5×106 cells were removed from the bioreactor and centrifuged at 200 x g for 2 min (VWR). The supernatant was discarded and the cell pellet was frozen in liquid nitrogen and stored at -80°C for further analysis.