Labshake search
Citations for VWR :
401 - 450 of 2348 citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover for 48 hours before passage into new 10 cm plates containing DMEM media supplemented with 2 mg/ml Geneticin sulfate (G418; VWR). Following 7 days of selection ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
-
bioRxiv - Physiology 2021Quote: ... and this portion was placed on top of a stack of 4-6 glass microscopy slides (depending on size of animal) (VWR, Radnor PA-Cat. No. 48300-026). Tissue was secured onto glass slide with a biorthogonal silicon elastomer (Kwik-Sil ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5% non-fat milk and 1% BSA filtered through a paper filter (qualitative, 415, VWR). Blots were rinsed 1-2 times with Wash Buffer ...
-
bioRxiv - Microbiology 2023Quote: ... 1 mL of swollen conidia was aliquoted into a 5 mL screwcap centrifuge tube (VWR). To each 1 mL aliquot ...
-
bioRxiv - Cell Biology 2023Quote: ... containing 1% Pen/Strep and 10% FBS (VWR International, Radnor, PA, #97068-085). For LLOMe treatment ...
-
bioRxiv - Synthetic Biology 2024Quote: Yeast extract Peptone Dextrose (YPD) media (10 g L−1 yeast extract (VWR), 20 g L−1 peptone (VWR) ...
-
bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
-
bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
-
bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Culture slants were made by pouring approximately 5-10 mL of PDYA at an angle into a 15 mL Falcon tube (VWR, Radnor, PA) and allowing the PDYA to solidify along the inner sidewall of the tube ...
-
bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
-
bioRxiv - Microbiology 2024Quote: ... smegmatis WT and ΔmftG strains were grown in 320 mL HdB-Tyl medium supplemented with 1 g L−1 ethanol as the sole carbon source in 2 L flasks covered by breathable rayon film (VWR) in triplicates and incubated at 37 °C and 210 rpm with a starting OD600 of 0.1 for WT and 2.0 for ΔmftG for a period of 48 h.
-
bioRxiv - Immunology 2019Quote: ... cells were resuspended in 1 mL of cold sorting buffer (L15/10 mM HEPES/1 mg/mL BSA (VWR)/25 μg/mL DNase I (Roche)) ...
-
bioRxiv - Microbiology 2020Quote: Calibration of the device was carried out by spraying deionized water on 9 cm diameter filter paper (Qualitative 415, VWR). In order to understand the relationship between pressure ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 3 ug of DNA in the same superfolder GFP-containing pEG BacMam vector used for large-scale purification and 9 μL FuGene HD (VWR) diluted in 150 μL OptiMEM and added dropwise to the cells after a 5 min incubation ...
-
bioRxiv - Microbiology 2022Quote: ... hexadec-9-enoic acid (P9417, MilliporeSigma), octadecanoic acid (S4751, MilliporeSigma), and pentadecanoic acid (P6125, MilliporeSigma) in ethanol (MACR6777-16, VWR), and 20% tyloxapol (T8761 ...
-
bioRxiv - Biochemistry 2023Quote: ... pH 9) was performed for 30 min using a water bath at 95 °C in a coplin jar (VWR Chemicals). Spheroid sections were blocked with Tissue Blocking Buffer (2% [v/v] normal mouse serum and 5% (w/v ...
-
bioRxiv - Pathology 2024Quote: Six μm sections of formalin-fixed paraffin-embedded (FFPE) tissue from the dorsolateral prefrontal cortex (Brodmann Area 9) were de-paraffinized with CitriSolv (Xylene substitute, VWR) for 20 min ...
-
bioRxiv - Immunology 2021Quote: ... BSA 5% (VWR) and fluorescently labelled antibodies at proper concentration ...
-
bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
-
bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
-
bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
-
bioRxiv - Neuroscience 2022Quote: ... or 2% BSA (2% Bovine Serum Albumin (VWR), 50mM Tris-Cl ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... EBY100 cells were thawed by inoculating 5 mL YPD (1% Bacto yeast extract (VWR #90000-726), 2% Bacto peptone (VWR #90000-368) ...
-
bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
-
bioRxiv - Microbiology 2020Quote: ... or after 10-1 dilution in molecular biology grade ddH20 (VWR Chemicals, Darmstadt, Germany) for specific amplification and high throughput sequencing of the fungal rDNA internal transcribed spacer 1 (ITS1) ...
-
bioRxiv - Microbiology 2020Quote: ... were co-transfected into cells at a ratio of 3:2:1 using PEI (VWR). After 16 h incubation ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50 mL PBS + 1 M NaCl and 50 mL 2 M Urea (VWR, 97063-798) + 50 mM Ammonium Bicarbonate ...
-
Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approachbioRxiv - Genetics 2022Quote: ... and these were plugged with 1/2 of a large cotton ball (VWR, 14224-518) dampened with 1-ml of 10% sucrose solution (Fisher Chemical ...
-
bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... The suspension was allowed to cool at room temperature (RT) for 10 min before adding 1 µl RNAse at 10 mg/ml (VWR cat no. 0675-250MG) and incubation at 37 for 60 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
-
bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
-
bioRxiv - Microbiology 2023Quote: ... The following day a 5mL layer of SMM top agar supplemented with hygromycin B (VWR) was added for a final concentration of 175ug/mL hygromycin for the entire 25mL SMM plate ...
-
bioRxiv - Plant Biology 2020Quote: One mL of anthocyanin extract was transferred to amber HPLC vials (VWR 46610-726). Samples were separated with a Dionex UltiMate 3000 UHPLC system and Phenomenex Kinetex F5 column (00D-4722-AN ...
-
bioRxiv - Molecular Biology 2023Quote: ... and baked for one hour at 60°C in a symphony™ incubator (VWR) from each of the tissue blocks ...
-
bioRxiv - Physiology 2023Quote: ... We used one of the 60 ml tick housing plastic vials (VWR, 216-1822P) with a 25 mm mesh-covered hole in the lid ...
-
bioRxiv - Genomics 2021Quote: ... 5% CO2 at a density between 0.3-1 x 106cells/mL in RPMI medium (VWR 45000-396) topped up with 10% Fetal Bovine Serum (Genesee Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... prepared as a 1:5 dilution in buffer (pH=7.2) made using Gurr buffered tablets (VWR #331942F) and filtered ...
-
bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
-
bioRxiv - Neuroscience 2024Quote: ... Microscope slides with serially sectioned nerves were rinsed in 1× PBS for 5 min (repeated twice) and air-dried by incubation at 70°C for 10 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
-
bioRxiv - Neuroscience 2021Quote: ... They were treated to a 1:1 mixture of 100% ethanol and methyl salicylate for 10 minutes before clearing and storage in methyl salicylate (VWR Chemicals).
-
bioRxiv - Neuroscience 2022Quote: ... The slices were immersed in a mixture of 100% ethanol and methyl salicylate (1:1, 10 minutes) before being stored in methyl salicylate (VWR Chemicals). Some slices were stored in a cryoprotective solution containing glycerol (20% ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 5 mM EDTA (VWR), and 0.1% sodium azide (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 5 mM EDTA (VWR), and 0.1% sodium azide (Sigma-Aldrich ...