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Citations for VWR :
351 - 383 of 383 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Plant Biology 2023Quote: ... Apogee grain were left for 2 days at 5°C in water for imbibition before being placed individually onto cotton in a 24-well tissue culture plate (VWR, USA) and left to germinate for 3 days under high humidity conditions (<90% relative humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... Tissues were sectioned onto gold slides (see below for more information) for MIBI analysis and SuperFrost glass slides (VWR, 48311-703) for GeoMx analysis ...
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bioRxiv - Neuroscience 2023Quote: ... spinal cords were incubated overnight with 50% Optimal Freezing Temperature (OCT) (Tissue-Tek, Cat No. 25608-930, VWR, Radnor, PA, USA) medium and 50% 20%-sucrose dissolved in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Four-micron sections of formalin-fixed paraffin-embedded tissues were mounted on positively charged Superfrost® Plus Slides (VWR, Radnor, PA, USA). The RNAscope® ISH assay was performed using the RNAscope 2.5 HD Red Detection Kit (ACD ...
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bioRxiv - Cell Biology 2020Quote: Gastrocnemii were homogenized with 25 strokes using a 5 mL PTFE tissue grinder with clearance 0.15-0.25 mm (VWR 89026-392, 89026-404) at 3,000 rpm in RIPA lysis buffer (VWR 97063-270 ...
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bioRxiv - Cell Biology 2019Quote: ... Fetal liver was dissected from the embryo and embedded in a 25-mm x 20-mm x 5-mm Tissue-Tek Cryomold (VWR, 25608-916) with optimal cutting temperature compound (Tissue-Tek O.C.T. ...
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bioRxiv - Cancer Biology 2019Quote: ... in RPMI with 5% FBS and 1% penicillin/streptomycin for 20 minutes in a humidified incubator at 37°C Tissues were washed with PBS and further dissociated in 0.25% Trypsin (VWR, Cat. # VWRL0154-0100) for 30 minutes in the incubator ...
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bioRxiv - Bioengineering 2020Quote: ... The tissue was sectioned using 40- to 60-μm slices and transferred onto a Superfrost Plus glass slide (VWR Inc., #48311-703). Sections were stored at −20°C before immunostaining.
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Molecular Biology 2020Quote: Adult hearts were dissected from Msk1/2 KO mice and covered in a layer of Tissue-Tek optimum cutting temperature (OCT) in Tissue-Tek® Cryomold® Molds (15×15×15 mm) and flash-frozen in liquid nitrogen-cooled isopentane (VWR). 10 μm ventricular sections were cut using a Leica cryostat and attached to SuperFrost Plus™ slides (VWR) ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... frozen and crushed in liquid nitrogen for protein and RNA extraction or ii) embedded in Tissue-Tek OCT compound (VWR, Mississauga, Canada) and frozen in isopentane cooled with liquid nitrogen for cryostat sectioning54 or iii ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
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bioRxiv - Cell Biology 2019Quote: ... 15 μl of tissue suspension was pipetted onto a well of a 10-well PTFE diagnostic slide (631-1371, VWR International GmbH) and left to dry completely ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was mechanically dissociated by gentle up and down pipetting using a 200 μl siliconized pipette tip (VWR cat No. 53503-794). The resulting suspension was seeded in small 20 - 50 μl droplets on ibiTreat μ-Dishes (Ibidi cat No ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Molecular Biology 2023Quote: ... Worms were resuspended in 7 ml pre-chilled Nuclei Purification Buffer+ and dounced on ice in a Wheaton dura-grind stainless steel dounce tissue grinder (VWR 62400-675) for 12 strokes ...
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bioRxiv - Genetics 2023Quote: ... Small pieces of cotton wool soaked with sterile water were placed in each well of a 24-well tissue culture plate (VWR International, US). One wheat seed was placed with the crease facing downwards into each well ...
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bioRxiv - Genomics 2023Quote: ... and their kidneys and brains were quickly collected and frozen immediately in optimal cutting temperature compound (Tissue-Tek O.C.T.; VWR, cat. no. 25608–930), before storing at −80 °C ...
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bioRxiv - Cell Biology 2023Quote: Lyophilized liver samples were homogenized in 10 mM phosphate buffer at pH 6 (28 mg tissue per ml buffer) on a bead beater (VWR, Radnor, PA) for 1 min ...
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bioRxiv - Cell Biology 2024Quote: Protein was collected by lysing worms in a 1:1 ratio of worm pellet to lysis buffer using a tissue grinder (VWR 62400-675) kept cold on ice ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 24 h starved polyps were incubated in 1 mM linalool for 10 min in a 60 mm tissue culture dish (VWR International, Radnor, PA). Each animal was pinched using a pair of Dumont No ...
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bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Physiology 2023Quote: ... Each brain tissue was carefully collected from the cranium individually and snap-frozen in a 1.5 mL microcentrifuge tube (VWR International, Radnor, PA, USA) chilled with liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... The tissue was cut into 1mm pieces and placed in cryomolds before being left to incubate in 361603E OCT (VWR International, Radnor, PA, USA) for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... (Lexogen SPLIT RNA kit) was added to each tube and the tissue homogenized using a plastic pestle (Kontes Pellet Pestle, VWR, catalogue number: KT749521-1500). Samples remained on ice to maintain RNA integrity while other samples were homogenized ...
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bioRxiv - Bioengineering 2021Quote: Fresh frozen lung lobe tissues with highest injury score (n=9) were homogenized using VWR® Mini Bead Mill Homogenizer (VWR International LLC., Radnor, PA) following manufacturer’s protocol ...