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Citations for VWR :
251 - 300 of 383 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Thin sections of tissue (10um) were mounted in 24 well glass bottom plates (82050-898, VWR) coated with a 1:50 dilution of APTES (440140 ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissues were washed 2X in TBST and developed with DAB (brown) Chromogen (VWR, 95041-478) for 5 min and washed again 2X in deionized water ...
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bioRxiv - Neuroscience 2022Quote: ... Stained tissue was mounted in SlowFade mounting medium on silane-coated microscopy slides (VWR #63411-01) and stored at 4°C ...
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bioRxiv - Immunology 2023Quote: Tissues were harvested following sacrifice via CO2 inhalation and fixed in 10 % formalin (VWR, Radnor, PA) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Brain tissue was mechanically homogenized by a cordless motor pellet mixer (#V8185-904; VWR, Mississauga, Canada) in a PBS-based cell lysis buffer complete with EDTA-free protease inhibitors (#04693159001 ...
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bioRxiv - Cell Biology 2022Quote: The adipose tissue was immediately perfused with PBS and fixed with 10% neutral-buffered formalin (VWR). Adipose tissues were then transferred to 70% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Developmental Biology 2024Quote: ... tissue samples were taken from webbing on hindlimbs using single-use biopsy punches (VWR 21909-140). Heterozygous mating pairs were intercrossed to produce embryos for in situ hybridization and RNA-seq analyses ...
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bioRxiv - Immunology 2024Quote: ... Brain was weighed and subsequently homogenized in iMED+ homogenized using a 2mL glass tissue grinder (VWR; 6 plunges ...
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bioRxiv - Immunology 2024Quote: ... The tissue suspension was then passed through 40 µm cell strainer (VWR international, Randor, PA, USA) and the cell suspension was collected ...
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bioRxiv - Cell Biology 2020Quote: ... Thin sections of tissue (10 ⎧m) were mounted in 24-well glass bottom plates (VWR 82050-898) coated with a 1:50 dilution of APTES (Sigma 440140) ...
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bioRxiv - Neuroscience 2022Quote: ... and ATG5 KO mice of both lines were homogenized using a Wheaton Potter-Elvehjem Tissue Grinder (VWR) in 6 M Urea/ 2 M Thiourea ...
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bioRxiv - Immunology 2019Quote: ... and plated at 1 ml/well in 24-well tissue culture plat (Falcon, VWR Edmonton, AB, Canada). Cells were stimulated with SLA (50 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Excised brain tissues were then homogenized in ice-cold nuclease-free water using Bead Mill homogenizer (VWR). Lipids were extracted from 7.5 mg of tissue homogenate containing 8 μl SPLASH LIPIDOMIX mass spec standard (Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from tissue or cell culture using the EZNA RNA isolation Kit (VWR, Spånga, Sweden) or using TRIzol reagent and RNeasy Universal Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... tissue was minced with a scalpel and further homogenized with the Potter-Elvehjem instrument (VWR, Darmstadt, Germany). The amount of tissue employed for each purification is documented in Supplemental Table S1 ...
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bioRxiv - Cell Biology 2021Quote: First trimester placental villous tissue (7-10 weeks of gestation) was snap-frozen in OCT compound (VWR) and 5µm cryosections of tissue were cut and fixed in ice-cold acetone for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Trichrome staining was performed on 5 μm tissue sections using Gomori’s Trichrome Stain Kit (VWR, 84000-308) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... After 24 hours tissues were embedded in Optimal Cutting Temperature (OCT) compound (VWR Chemicals, Atlanta, GA, USA) and snap frozen on a metal bar cooled by dry ice ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mouse brains and flank tumors were homogenized in a pre-chilled glass tissue grinder (VWR) with RIPA lysis buffer containing protease and phosphatase inhibitors (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... For direct RNA isolation the lung tissue was transferred into Precellys beads CK14 tubes (VWR, 10144-554) containing 1ml 900 μl Qiazol (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was embedded in VWR® Clear Frozen Section Compound (VWR International LLC, Radnor, PA, US) and sectioned coronally at 40 or 50 μm using a Leica cryostat set to −21°C (CM 1850 UV ...
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bioRxiv - Microbiology 2023Quote: ... and tissue was mechanically disrupted by beads to further lyse bacterial cell walls (VWR Mini Bead Mill).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Organoids were fixed in 4% PFA and embedded in Tissue-Tek® O.C.T (VWR, cat # 25608-930). Slides of cryosectioned organoids were blocked in 5% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Neuroscience 2023Quote: ... sequential cutting of 50µm tissue sections were mounted onto a SuperFrost Plus Microscope Slide (VWR International; UK). Slides were washed 3 times with PBS for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Cancer Biology 2024Quote: ... at a cell density of 4.166×105 cells/mL in tissue culture-treated T-75 flasks (VWR) (7) ...
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bioRxiv - Molecular Biology 2021Quote: ... Thin sections of tissue (10μm) were mounted in 24-well glass bottom plates (VWR International, LLC, 82050-898) coated with a 1:50 dilution of APTES (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and surrounding with silicon grease (to protect tissue from high pressures) before applying glass coverslips (VWR, 631-1574) and leaving for 48 hours to set (Figure 6b) ...
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bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
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bioRxiv - Neuroscience 2022Quote: ... slices were homogenized in 1.5 mL 8 M Urea buffer using a Wheaton Potter-Elvehjem Tissue Grinder (VWR). The lysates were sonicated and centrifuged at 13000 rpm for 10 min ...
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bioRxiv - Biochemistry 2019Quote: ... The dissociated brain tissue samples were gently homogenized (20 strokes) with a glass-Teflon homogenizer (# 89026-384 VWR). The homogenized tissue samples were filtered with 40 μm mesh filter (# 22-363-547 Fisher scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... Immunostained tissue was mounted on positively charged glass slides (41351253, Worldwide Medical Products) and coverslipped (48393-195, VWR) with Fluoromount-G mounting medium (100241-874 ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
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bioRxiv - Neuroscience 2023Quote: ... on glass microscope slides with parafilm spacers to prevent tissue distortion and overlaid with coverslips (VWR, #631-0147).
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bioRxiv - Cell Biology 2023Quote: ... The tissue blocks were cryosectioned (20 µm) and mounted on microscope slides (Superfrost Plus Adhesion Microscope slides, VWR) and stored at -80°C ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then sectioned on a Leica cryostat at 20 μm onto Superfrost plus microslides (VWR, 48311-703) and stored at −80 °C until use.
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bioRxiv - Cell Biology 2023Quote: ... Digested tissues were passed through 100 and 70 um nylon cell strainers (Corning, VWR# 10054-458, 89508-344). Single cell suspensions were washed by MACS buffer ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Cells and tissues were lysed by radioimmunoprecipitation assay (RIPA) buffer (0.2% IGEPAL CA-620, 0.02% SDS with VWR Life Science Protease Inhibitor Cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... Mucosal CFU counting was achieved by plating series of dilutions of homogenized colonic mucosa (0.5 cm tissue sections, weighted and processed in 500μL 1X PBS with Bead Mill Homogenizer, VWR). Blood CFU counting was performed by plating 50μL of blood collected in the presence of an anticoagulant (citrate) ...
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bioRxiv - Physiology 2024Quote: ... Pancreases were then preserved in optimal cutting temperature compound (Tissue-Tek O.C.T. Compound, VWR, catalog number 25608-930) and stored in -80°C for serial sectioning on a later date.
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bioRxiv - Neuroscience 2024Quote: ... with all SGZ-derived NSCs transferred to Sterile tissue culture treated 6-well plate (VWR, Cat# 10861-696) at 10 DIV following P1 passage ...
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bioRxiv - Neuroscience 2024Quote: ... The current waveforms were applied to the tissue via a 0.25mm diameter platinum wire electrode (VWR, Lutterworth, UK) positioned 50-100μm from the recorded neuron ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Immunology 2021Quote: Single-cell leukocyte suspensions were prepared from spleens by mechanical homogenization of tissues between frosted glass microscope slides (VWR) and filtration through a 70µm nylon mesh ...
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bioRxiv - Neuroscience 2021Quote: ... and cryopreserved by 24-48 hour incubation at 4oC in 30% sucrose diluted in PBS (pH=7.4) and embedded in a mixture of OCT (Tissue-Tek, VWR) and 30% sucrose ...