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Citations for VWR :
251 - 285 of 285 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and excess gel was washed away with 5 Milli-Q purified water washes and 5 Dulbecco’s Phosphate Buffered Saline (DPBS) solution (VWR L0119-0500) washes ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Molecular Biology 2024Quote: Arabidopsis thaliana Col-0 seeds were surface sterilized for 10 min in a 50% bleach solution with Tween 20 (VWR 0777A) followed by three washes with sterile dH2O ...
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bioRxiv - Microbiology 2024Quote: ... The pigmented extract was dissolved in 99.5% methanol and then 10 mL of the solution was poured into a clean cuvette and inserted into a P9 double beam UV-visible spectrophotometer (VWR, China) which directs a beam of light through the sample using methanol as negative control ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... a resazurin solution was prepared by dissolving one tablet of Resazurin (Resazurin Tablets for Milk Testing; Ref. 330884Y VWR International Ltd) in 30 mL of sterile PBS (phosphate buffered saline) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2022Quote: ... 50 µL of the agarose solution was then transferred over to each well of the 96 well U bottom plate (10062-900, VWR, PA, USA) then let solidify at room temperature (RT) ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Bioengineering 2024Quote: ... the peptide solutions were pH adjusted to 7 and sterile filtered using 0.2 μm cellulose acetate sterile syringe filters (VWR International, Radnor, PA) under sterile conditions ...
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bioRxiv - Bioengineering 2022Quote: ... cells for both batches were seeded onto the material at a confluence comprised between 50% and 60% on fibronectin coated (15 μg/ml in PBS buffer solution) 18-mm round glass coverslip (VWR, Radnor, USA). The cells were maintained in incubator at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903], 10mM KH2PO4 [VWR, 470302] ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Bioengineering 2024Quote: ... was defined by completely demineralizing a 2% gelatin solution by adding an abundance of mixed bed ion exchange resin Amberlite IRN-150 (VWR, 40992.A9) to the gelatin ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was passed through a 0.22 µm filter before 0.5 ml was combined with 2 ml of Patton-Reeders solution (0.105 mM calconcarboxylic acid, VWR, in 0.73 M NaOH) and vortexed briefly to mix ...
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bioRxiv - Plant Biology 2021Quote: ... Membrane protein samples containing 1 μg total Chl were solubilized and separated by electrophoresis on a 10 % SDS-polyacrylamide gel using Next Gel solutions and buffers (VWR, Radnor, PA, USA). Proteins were transferred to Immobilon-P PVDF membranes (MilliporeSigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumors were weighed and rinsed in blood bank saline solution (150 mM NaCl) and blotted on filter paper (VWR, Radnor, PA, 28298–020). The process of dissection and tumor preparation took < 3min ...
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bioRxiv - Neuroscience 2023Quote: ... slides that included the region of interest were washed with PBS before quenching with a solution of 50% methanol (VWR, Radnor, PA, USA) and 3% hydrogen peroxide (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Cell Biology 2024Quote: ... cells were passaged 1:10 to a new 96-well Matrigel-coated plate for gDNA extraction with 20 μL of Quick Extract gDNA lysis solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA). The remaining cells were overlaid with 50 μL of 20% mTeSR1 ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder of the cells were spun and resuspended in 20 μL of Quick Extract gDNA lysis solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA) and prepared for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... and stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was extracted from lysed samples using the Quick Extract DNA Extraction Solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA), following the protocol provided by the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: ... the tubes and multiplexers of all liquids are flushed for cleaning with first 1.4 % sodium hypochlorite in aqueous solution (7681-52-9, VWR International, Radnor, PA, USA) diluted with ultrapure water ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Microbiology 2021Quote: ... They were then stained with 2% uranyl acetate in an aqueous solution (Taab Laboratories, Berkshire, UK) for 15 minutes followed by Reynolds lead citrate stain (VWR International Ltd, Leicestershire, UK) (Reynolds ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −20 °C in an anti-freeze solution (30 % glycerol [Merck Millipore, Germany], 30 % ethylene glycol [VWR International, USA] and 10 % TBS) until further processing ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were first incubated for 1 h in blocking solution (0.01 M PBS with 0.3% Triton X-100, Sigma, T9284; 5% normal donkey serum, NDS, VWR, 80054-446 or Calbiochem, 566460-5ML) at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Tissues were dehydrated using an ascending methanol series and bleached overnight at 4 °C in a solution containing 5% H2O2 (VWR Chemicals, Radnor, PE, USA) in methanol ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed midguts were blocked for 1 h in blocking solution (1% bovine serum albumin, Cat# A8806, Sigma-Aldrich, St. Louis, MO; 1% normal donkey serum, Cat# 102644-006, VWR, Radnor, PA. in PBS). Then the primary antibody (1:1000 ...