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Citations for VWR :
201 - 250 of 285 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated by incubating the RNA samples in a solution containing qScript cDNA SuperMix (VWR #101414-102) and nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and fixed/stained in crystal violet/methanol solution (25% (v/v) methanol (VWR #20847.307) + 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... the canary underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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bioRxiv - Immunology 2023Quote: ... then placed overnight at 4°C in a 25% sucrose solution containing 50% optimal cutting temperature compound (OCT, VWR Clear Frozen Section Compound ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were collected onto copper mesh grids and contrasted for 2min with 4% uranyl acetate solution in methanol (VWR), followed by 2min in lead citrate (Reynold’s solution) ...
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bioRxiv - Bioengineering 2023Quote: ... Then 10 μL of fluorescein sodium aqueous solution was added to the particles and mixed well with vortex (VWR). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then blocked in a solution containing 5% skimmed milk powder in Tris-buffered saline with 0.1% Tween-20 (VWR) for 1 hour at ambient temperature.
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bioRxiv - Immunology 2024Quote: ... The solution was filtered into a new Eppendorf tube through a 100 µM cell strainer (Falcon/VWR #21008-949). The epithelial cells were then spun down at 1000 g for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal sections (300 μm thick) were cut in the same solution using a vibratome (Vibration microtome, HM 650V, VWR) and afterwards kept in ACSF (in mM ...
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bioRxiv - Cell Biology 2024Quote: ... an agarose mounting pad (10% agarose in M9 buffer62 was prepared prior to imaging by briefly heating the agarose solution and flattening it between two slides (#1.5, VWR). Approximately 3 µL 0.1 µm Polybead microsphere suspension (Polysciences ...
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bioRxiv - Biophysics 2021Quote: ... 2 drops (~30 μL) of 2% melted agarose solution (in water) were dropped to glass slides (VWR Vistavision #16004-422) and flattened by placing another slide on top with thin (~0.2 mm ...
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bioRxiv - Developmental Biology 2020Quote: ... Eyes were then incubated in a 1:1 solution of OCT:30% sucrose in PBS for one hour and frozen in OCT (VWR). The frozen tissues were sectioned using a Leica CM1850 cryostat for collecting serial retinal sections of 16μm thickness ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Neuroscience 2020Quote: ... and dried gently with Kimwipes and transferred to a refractive index matching (RIM) solution of 63% (v/v) 2,2′-thiodiethanol (TDE, VWR international) in 10 mM PBS ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% Fast green (Raymond A Lamb) (both in distilled water) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Bone marrow isolation and cell staining was performed on ice in staining medium (SM; Hanks Balanced Salt Solution [HBSS 1x, Corning 1-022-CM] supplemented with heat inactivated fetal bovine serum [FBS, VWR 97068-05] to a final concentration of 2%) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We removed nearly all dissection/fixation solution from the sample and picked up the coverslip with a HistoBond microscope slide (VWR). Samples were then frozen on dry ice ...
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bioRxiv - Bioengineering 2022Quote: ... the inactivating solution was replaced by pre-warmed plating medium (10% horse serum (#S900-500, VWR International GmbH, Darmstadt, Germany), 1.8 mM glutamine ...
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bioRxiv - Microbiology 2022Quote: ... after electrophoresis gels were immersed into a methanol/sodium acetate (pH=5) solution and incubated at room temperature on rocking platform (VWR) at 1 speed for 2 min with constant shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Piranha solution reacts violently with organic matter and must be handled and disposed of according to institutional guidelines! Coverslips (VWR) were placed in a glass container and freshly prepared piranha solution was added ...
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bioRxiv - Microbiology 2023Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Immunology 2023Quote: ... Analytical grade ammonia solution (28%, GPR RECTAPUR®) and acetic acid glacial (AnalaR® NORMAPUR®) were obtained from VWR Chemicals.
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Label-free digital holotomography reveals ibuprofen-induced morphological changes to red blood cellsbioRxiv - Cell Biology 2022Quote: ... Ibuprofen powder was obtained by crashing a 400 mg ibuprofen tablet and stock solutions were prepared by dissolving ibuprofen (2 mg/mL) in 2 mL of PBS (VWR). Four concentrations of ibuprofen solution were prepared (0.25 mM ...
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bioRxiv - Microbiology 2023Quote: ... gels were immersed in a methanol-sodium acetate solution (pH 5) and incubated at room temperature on a rocking platform (VWR) at a speed of 1 for 2 min ...
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration in KGM was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). Cells were maintained at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). For all hTCEpi cell experiments ...
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bioRxiv - Neuroscience 2024Quote: ... slides were warmed to room temperature prior to antigen retrieval in a 1X antigen retrieval solution (VWR, Cat# 103780-314) for 5 min at 80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were formed by dripping the cell suspension into a sterile 100 mM CaCl₂ solution (VWR, Fontenay-sous-Bois, France). The beads were subsequently washed in 0.15 M NaCl solution (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... CV was measured in 1 mM [Fe(CN)6]3- in 1 M KCl solution (VWR, catalog no. BDH9258-500G) at a scan rate of 200 mV/s.
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bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed tissue was placed in sucrose solution (30% sucrose in PBS) for 24h and frozen in tissue mounting medium (OCT mounting media, VWR chemicals). The tissue was coronally sectioned at 50-60µm thickness on a cryostat ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was then incubated in 4% PFA/PBS overnight and either immersed in serial solutions of 15% and 30% sucrose/PBS and embedded in OCT (VWR Chemicals) for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica) ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... fixed breast samples were impregnated with 30% Sucrose solution in 1X PBS for at least 48h and embedded in OCT (VWR, #361603E). 70 μm-thick sections were incubated with 0.5% Triton-blocking buffer (5% FBS and 2% BSA in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Developmental Biology 2020Quote: ... each embryo was positioned in a sagittal orientation in a tissue base mold (Sakura, 4162) in optimal cutting temperature compound (OCT) solution (Sakura, 4583) and frozen in a dry ice isopropanol (VWR, 20842) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Cancer Biology 2021Quote: Glass-bottom 6-well dishes (#0 thickness, IBL, #220.200.020) were treated with a bind-silane solution consisting of PlusOne Bind-Silane (VWR, #17-1330-01) and acetic acid (Pancreac Quimica ...
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bioRxiv - Neuroscience 2023Quote: ... brains were kept for 1 hour in a 1:1 solution of 30% sucrose and Optimal Cutting Temperature Medium (OCT, VWR International) at 4°C ...