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Citations for VWR :
251 - 300 of 329 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Neuroscience 2024Quote: ... a 1-3 day old virgin female was placed in a 5.0 ml culture tube (VWR, Cat. No. 60818-565) in an ice bath for approximately 30 seconds until the fly was completely immobilized ...
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bioRxiv - Cell Biology 2024Quote: ... BLOC-1-deficient melan-mu melanocytes were plated at 3 x 104 cells/ml in 35 mm glass bottom dishes (VWR) in the presence or absence of 20 µM forskolin for 6 days ...
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bioRxiv - Synthetic Biology 2024Quote: ... At least 3 colonies per strain were picked and inoculated in 2 mL-deep 96 well plates (VWR 76397-576) with 1 mL of MPG supplemented with 50 μg/mL ampicillin and 100 μL of 20% dextrose ...
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bioRxiv - Cancer Biology 2024Quote: ... Excess DAPI was cleared with 3×5 min washes in PBS and slices were then mounted on glass slides (VWR) using a ProLong Gold antifade reagent (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... CV was measured in 1 mM [Fe(CN)6]3- in 1 M KCl solution (VWR, catalog no. BDH9258-500G) at a scan rate of 200 mV/s.
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...
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bioRxiv - Microbiology 2024Quote: ... the transgenic gametocytes were pelleted at 1,400 xg for 3 minutes and fixed in 4% v/v paraformaldehyde (VWR, Cat# PI28908) and 0.0075% v/v glutaraldehyde (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mM imidazole and 200 mM NaCl was added before loading on a self-packed 3 ml Ni Sepharose™ 6 Fast Flow (VWR) column using ÄKTA Pure FPLC system (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... for 50 ms at 950 ms intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Biochemistry 2023Quote: ... whereas other enzymes were removed by passing the sample through a 3 kDa NanoSep centrifugal filter device (VWR, Radnor, PA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Microbiology 2024Quote: ... DBS samples were made by spotting 8 μL of sample onto Whatman grade 3 qualitative filter paper (VWR, cat #1003-917) cut to 96-well plate size ...
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bioRxiv - Cancer Biology 2024Quote: The SKOV-3-luc ovarian cancer cell line was harvested and washed with Dulbecco’s phosphate-buffered saline (DPBS, VWR, Oslo, Norway), and the cell number and viability were determined using a Countess Cell Counter (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Microbiology 2021Quote: ... and cleared refolded StaR was supplemented with additional 2 mM DTT and concentrated using a centrifugal filter with a MWCO of 3 kDa (VWR #516-0227P) and stored at −80 °C ...
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... a 3 mg/mL ORO stock was prepared by dissolving ORO powder in 100% 2-proponal (VWR #BDH2032-1GLP, Radnor, PA, USA). That stock solution was then diluted to a 1.8 mg/mL working solution in UltraPure™ Distilled Water (ThermoFisher #10977015) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2% FBS were injected into the left ventricle of the mice with a 26G × 3/8” needle attached to tuberculin syringe (VWR, Cat: BD309625). During the course of colonization assays ...
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bioRxiv - Bioengineering 2024Quote: ... An incision was made above the quadriceps and either a 2 mm (for sub-critical VML analysis) or a 3 mm (for critical VML analysis) biopsy punch (VWR, 21909-136) was used to make a full-thickness muscle defect28 ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were then mechanically sleep-deprived 3 sec every min from ZT12-ZT0 using a vortexer (VWR DVX-2500 Multi-tube vortexer). Flies were then taken out of the individual tubes between ZT23 and ZT0 and placed into standard imaging chambers using a mouth pipette ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Microbiology 2024Quote: ... Enzyme solution was prepared in distilled water and the protein concentration was determined using Bradford reagent (VWR, USA) with bovine serum albumin as the protein standard ...
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bioRxiv - Cell Biology 2024Quote: 25 µg of proteins were separated in SDS-PAGE gels before being transferred to nitrocellulose membranes (VWR 10600002). After ponceau staining ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min each time, cryopreserved in 30% sucrose (S5-3, Fisher Chemical) in PBS overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (12 μm ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant was removed and replaced with 500 μL of ACK (ammonium-chloride-potassium) lysis buffer per sample to remove red blood cell contamination (3 min incubation at 37°C; VWR cat#10128-808). Reaction was quenched with 10 mL of cold DMEM/F12-Advanced media supplemented with 10% FBS,1% L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed off the filter with an ice-cold freshly prepared mixture of methanol/ethanol/chloroform (1:3:1) (ethanol LiChroSolv©, Merck, Darmstadt, Germany; chloroform HiPerSolv©, VWR, Dresden, Germany). Extracts were treated in an ultrasonic bath for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Four sets of dilutions (100 to 10-3) of the filtered lysates were created in standard 96 well plates (VWR, cat. no. 25381-056), then arrayed in quadrants of a single 384-well plate ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...