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Citations for VWR :
151 - 200 of 329 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The sample-beads were then washed with 3 mL 75-80% ethanol (VWR Chemicals, PA, USA) and again with 2 mL 75-80% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... with 100 μg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 μM CdCl2 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were spun and then incubated at 91°C for 3 min on heat blocks (VWR), then 60°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (3 x 5 min) and mounted on slides (Superfrost Plus, VWR) with mounting medium (Fluoromount ...
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bioRxiv - Immunology 2024Quote: ... Endogenous peroxidase activity was blocked with a 5-minute incubation in 3% hydrogen peroxidase (VWR, 23614.291) and further by treating the samples with Bloxall blocking solution (SP-6000 ...
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Physiology 2024Quote: ... the 96-well plate was centrifuged at 3800 rpm for 3 min at 4°C (VWR® Benchtop General Purpose Centrifuges ...
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bioRxiv - Bioengineering 2024Quote: ... 200 mg of paKG polymers were dissolved in 3 mL of dichloromethane (DCM) (VWR, Radnor, PA). 10 mg of PFK15 (Selleckchem ...
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bioRxiv - Immunology 2024Quote: ... with media changes every 2-3 days before expansion to a T-75 flask (CellStar, VWR).
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bioRxiv - Bioengineering 2024Quote: ... at passage number 3 were cultured to ∼90% confluency in 6-well plates (734-2323, VWR) in EGM-2 medium (CC-3162 ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Neuroscience 2024Quote: ... or RNA and protein extraction (18 mm cover glasses [631-0153; VWR] in 12 well plates [142475 ...
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bioRxiv - Genomics 2024Quote: ... antibody overnight followed by incubation with protein A coupled Sepharose beads (VWR). Samples were washed with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein induction was carried out in bacteria grown in Terrific Broth (VWR) medium with 1mM IPTG (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Plant Biology 2021Quote: ... 3.5 mL of 18.2 MΩ deionised water (Elga PURELAB flex 3 system, VWR International, Edmonton, AB, Canada) was then added to effect a separation into a polar methanol/water layer and a chloroform layer ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
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bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
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bioRxiv - Neuroscience 2022Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
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bioRxiv - Microbiology 2024Quote: ... Moore swabs were constructed by folding 3 by 24-inch strips of cheese cloth (VWR, Radnor, PA) 8 times to form an 8-ply square pad ...
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bioRxiv - Bioengineering 2024Quote: ... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
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bioRxiv - Cancer Biology 2024Quote: PDAC-3 spheroids were grown in VWR®96-wells F-bottom plates (VWR, Cat No. 7342327) coated with 1.5% w/v agarose ...
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bioRxiv - Biochemistry 2024Quote: ... and inoculated on a Nutrient Agar plate containing 100 μg/mL ampicillin (VWR; Cas: 69-52-3). After culturing overnight at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were washed from 3 to 5 times for 10 min in PBS-Triton 0.8% (28817295, VWR) and permeabilized in 0.25% Trypsin-EDTA (sc391060 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor growth was monitored weekly and tumor volume was measured along 3 axes with calipers (VWR 62379-531). Tumors and lungs were harvested at time of tumor burden (total tumor volume of 2cm3 ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at room temperature on a shaker in presence of 3 μg LysC Endopeptidase (VWR). Next ...
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bioRxiv - Genomics 2020Quote: ... were co-transfected with the respective lentiviral expression vector (∼1:1:3 molar ratio) using Trans-IT (VWR) and OptiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μm-thick sections were obtained and counterstained with Liquid-Stable Nuclear Fast Red (VWR Life Science AMRESCO).
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
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bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer; VWR, Radnor, PA, United States). The supernatant was transferred to a clean tube ...
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bioRxiv - Microbiology 2024Quote: ... were added by incubating samples for 3.5 hours at 37°C in 3% BSA (VWR Cat# 97061-422) in PBS ...
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bioRxiv - Genetics 2023Quote: The muscarinic receptor antagonist atropine ((RS)-(8-Methyl-8-azabicyclo[3.2.1]oct-3-yl, VWR Cat. TCA0754-005G) was dissolved in ethanol and added to 1M (HS ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min in 1x PBS and mounted onto SuperFrost slides (VWR, #48311- 703) using VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories ...