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Citations for VWR :
201 - 250 of 294 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in blocking buffer consisting of 5% bovine serum albumin (BSA; VWR) dissolved in 1X Tris-buffered saline (TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then centrifuged and the supernatant filtered through a 0.22 μm PES membrane (Avantor, VWR). For each clone a maximum growth rate was estimated from 5 measurements of optical density (OD ...
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bioRxiv - Cell Biology 2024Quote: ... the lentivirus was filtered through a cellulose acetate membrane (0.45 µm, #28145-481, VWR), and 250 µl of media containing the lentivirus were added to a 60mm dish of the indicated cells along with polybrene (8 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins separated on SDS-PAGE gels were transferred to nitrocellulose membranes (VWR, #27376-991) and then probed with appropriate antibodies ...
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bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of minceate was then suspended in 1 mL ice-cold 0.3 M sucrose (Fisher, cat# BP220) in H2O supplemented with protease inhibitor cocktail (VWR, cat# M250) at 1:100 (1:10 w/v suspension) ...
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bioRxiv - Biophysics 2021Quote: ... The chamber was filled with 500 μl Swelling Buffer (1 mM HEPES, 0.25 M sucrose, 1 mM DTT) and sealed with Critoseal (VWR International, Radnor, PA). GUVs were formed by applying a 1.8 V sinusoidal voltage at 10 Hz for at least 2 h at room temperature.
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bioRxiv - Microbiology 2020Quote: Microspheres were washed once with deionized water and incubated for 20 minutes in an end-over-end rotator in the dark in a suspension of 0.1 M NaH2PO4 (VWR; Radnor, PA, USA), 5 mg/mL EDC (1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2022Quote: ... lumbar spinal cords were cut (30 μm thickness) in series in the transverse plane with a cryostat (Bright Instruments, UK) mounted onto charged glass slides (VWR, #631-0108), and stored at −20°C ...
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bioRxiv - Immunology 2020Quote: ... Microspheres were washed once with deionized water and incubated for 20 minutes in the dark in an end-over-end rotator suspension of 0.1 M NaH2PO4 (VWR; Radnor, PA, USA), 5 mg/mL EDC (1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Developmental Biology 2024Quote: ... We cut blocks into 5-6 μm sections on a Shandon Finesse Me+ microtome (cat. no. 77500102) with High-Profile Disposable Surgipath DB80 HS Blades (VWR, 10015-022), and collected sections on Apex Superior Adhesive slides (Leica Microsystems ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was passed through a 0.22 µm filter before 0.5 ml was combined with 2 ml of Patton-Reeders solution (0.105 mM calconcarboxylic acid, VWR, in 0.73 M NaOH) and vortexed briefly to mix ...
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bioRxiv - Developmental Biology 2024Quote: ... was conducted in 1x Towbin buffer with 0.1 % SDS for 30 minutes (25 mM Tris-base, 192 mM Glycine, 1 % SDS) using a PerfectBlue Semi-Dry Electroblotter Sedec M (VWR; 700-1220). Transfer was performed at a constant current of 10 mA for 90 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... SIH5841.5), 1960 µL of deionized (DI) H2O (PURELAB Flex, ELGA LabWater) and 120 µL of 0.5 M HCl (VWR, catalog no. 20246.298). The solution was stirred vigorously for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... a 24-well insert with an 8-μm-pore membrane Trans-wells (VWR International Ltd) was coated with 50 μl of CellMatrix/PureCol collagen mixture (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... sections were transferred to 0.4 μm polycarbonate membranes cell culture inserts (VWR, Radnor, PA, USA) and incubated in Claycomb medium supplemented with 10 % foetal calf serum ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were blocked for 30 minutes with either 5% milk or BSA (VWR, 97061-416) diluted in 1 X TBST [100 mL of 10 × 1L Stock (90 g Tris Base (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatant was passed through a syringe filter (0.2 μm cellulose acetate membrane, VWR). The filtrate was kept frozen for 48 hrs at −20°C and then thawed and centrifuged (3000 x g ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with 5 % milk powder (MP, Skim milk powder, (VWR, Radnor, PA, US)) in 1x TBST buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then imaged on a C-DiGit Chemiluminescent Western Blot Scanner (103375-240, VWR) using SuperSignal West Pico PLUS Chemiluminescent Substrate (PI34577 ...
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bioRxiv - Cancer Biology 2023Quote: ... or 6.5 mm cell culture inserts (8.0 µm pore and polycarbonate membrane, VWR #734-2744). 2 hours prior to the assay ...
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bioRxiv - Physiology 2024Quote: ... sterilized using a vacuum filtration system with polyethersulfone (PES) membrane (2 μm pore size; VWR), and stored at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... the collected supernatant was passed through triple layered nylon membranes (150 and 50 μm, VWR) and then through an 8 μm pore size filter (Millipore ...
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bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
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bioRxiv - Plant Biology 2020Quote: ... plants were harvested and meristems were stained with 5 μM SynaptoRed membrane dye (VWR 80510-682) in DMSO ...
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bioRxiv - Bioengineering 2021Quote: ... The solution was then transferred into 12-14 kDa MWCO dialysis membranes (VWR, Chicago, IL, USA) and dialyzed against DI water for one week ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was UV-crosslinked to the membrane by using VWR UV crosslinker (VWR, #89131-484) at 120,000 microjoules per cm2 and 100 ng/mL of the probe diluted in DIG Easy Hyb Granules Working Buffer was hybridized overnight at 42°C with gentle agitation (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... A transwell plate insert with a 0.4µm pore size bottom PET membrane (VWR, Cat# 76313-906) was placed in each well and the inoculum (MOI=5 ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting blend was then filtered through a cellulose acetate membrane (0.45 µm, VWR, #28145-481). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal volume (2.5 µl) of purified RNA was dotted on Amersham Hybond N+ membrane (VWR) and crosslinked using an UV Stratalinker 2400 (Autocrosslink mode) ...
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bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... Bacteria were lysed using lysis buffer (25 mM Tris-HCl [Sigma-Aldrich], 1 M NaCl [Sigma-Aldrich], 10 µM EDTA [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were first incubated for 1 h in blocking solution (0.01 M PBS with 0.3% Triton X-100, Sigma, T9284; 5% normal donkey serum, NDS, VWR, 80054-446 or Calbiochem, 566460-5ML) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 5% non-fat milk or 5% bovine serum albumin (BSA; VWR #97062-904) in TBS before probing overnight at 4°C with primary antibodies diluted in TBS containing 0.2% Tween-20 (TBS-Tw) ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with goat anti-rabbit IgG conjugated to HRP (VWR, cat. no. 102645-182) secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 15,000 rpm for 2 min and filtered through a 0.2 μm cellulose acetate membrane (VWR). Then ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was transferred using a semi-dry system to a Nytran SuPerCharge nylon blotting membrane (VWR). After transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR, cat. no. 102646-160) and goat anti-rabbit IgG conjugated to HRP (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR, cat. no. 102646-160) or goat anti-rabbit IgG conjugated to HRP (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked by 5% Powdered milk (Roth, T145.3) in PBS-0.2% Tween20 (VWR Life Science, M147-1L), detected with the indicated antibodies and analyzed by quantitative near-infrared scanning (Odyssey ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a 500uL volume of each culture was filtered-sterilized (0.2 um centrifugal filter; nylon membrane; VWR 82031-358) and assayed in technical triplicate for enzymatic starch hydrolysis (see measuring amylolytic function below) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Zoology 2021Quote: ... the samples were passed through a 5–15 μm pore size paper membrane (VWR, Cat No. 516-0813) by gravity filtration to avoid damaging the cells– M ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was cleared by centrifugation at 20,000g for 30 min and filtered through a 0.45 µm cellulose acetate membrane (VWR). In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was lysed with TRK lysis buffer of the Micro Elute Total RNA Kit (VWR International PBI) and left rotating on a wheel for 20 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... the collected culture medium was filtered with a 0.45 μm polyethersulfone (PES) membrane (VWR International, Radnor, PA, USA) to avoid contamination by the cellular proteins ...