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Citations for VWR :
151 - 200 of 294 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and then transferred onto a Biotrace NT nitrocellulose membrane (27376-991, VWR). Total proteins were stained with Ponceau S solution (P7170 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... to the membrane and exposing it to autoradiography film (VWR 490001-930).
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 1X PBS (prepared from 10X concentrate; VWR International) containing 0.05% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked in 1X PBS (prepared from 10X concentrate; VWR International) containing 0.05% (v/v ...
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bioRxiv - Physiology 2023Quote: ... membranes were cut to remove excess and Ponceau S stained (VWR K793) to confirm complete transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Resulting solution was filtered through a centrifuge filter (30K PES membrane, VWR) for 7 min at 12,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was crosslinked to membranes at 2400J/m2 using UV Crosslinker (VWR) and tRNA were detected using digoxigenin-labeled (DIG ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Microbiology 2021Quote: ... We mixed 30 μl of each lysate with 200 μl UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 μl centrifugal filter unit (VWR International) and centrifuged the mixture at 14,000 x g for 40 min ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed by placing the plates on a shaker after adding 250 μL 0.1 M NaOH (VWR, US). Aliquots of 150 μL were pipetted out of each well into a scintillation bottle (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Microbiology 2023Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... lysate and UA solution were mixed in a 3:10 ratio (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µL centrifugal filter unit (VWR International) and the mixture was centrifuged at 14,000g for 40 min ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Microbiology 2024Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... we combined 60 uL of the lysate supernatant with 400 uL UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µl centrifugal filter unit (VWR International) and centrifuged for 40 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was immediately and gently mixed with 1 ml NCM medium containing 0.38 M mannitol and recovered in a round bottom tube (VWR) at 37 °C on a rotary shaker (120 rpm ...
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bioRxiv - Developmental Biology 2021Quote: ... and sealed around the membrane borders with oil 10 S Voltalef (VWR Chemicals). Images were acquired with 60x oil objective (NA 1.4 ...
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bioRxiv - Microbiology 2021Quote: ... and transferred to membrane (Nytran SuPerCharge) using the TurboBlotter system (VWR 89026-838). A DNA probe consisting of the 5’ 750 bp of the PPS gene was produced by PCR (primers 16/17) ...
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bioRxiv - Cell Biology 2020Quote: ... and sealed around the membrane borders with oil 10 S Voltalef (VWR Chemicals). Images were acquired with 60x oil objective (NA 1.4 ...
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bioRxiv - Systems Biology 2022Quote: ... and flowed them through a 0.2 µm pore filter (VWR, cellulose-acetate membrane). We then directly proceeded to measure the ROS production rate in the supernatant as described in the paragraph “measuring extracellular ROS production rate”.
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bioRxiv - Pathology 2021Quote: ... then the supernatants were filtered through a 0.2 μm cellulose acetate membrane (VWR) into sterile 1.5 mL Eppendorf tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... or 10% SDS-PAGE and wet transferred to Nitrocellulose membranes (VWR, CA10061-084) at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... filtration through a polyethersulfone (PES) membrane filter with 0.45 µm pore size (VWR), Ultracentrifugation at 4°C and 120,000 xg for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: Membranes were blocked with the LI-COR Biosciences Intercept Blocking buffer (VWR International) for one hour at room temperature and incubated with primary antibodies overnight at 4 °C on a rocker ...
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bioRxiv - Molecular Biology 2024Quote: ... Nitrocellulose membrane was then incubated with goat Anti-mouse (102971-154, VWR International) and goat Anti-rabbit (103011-498 ...
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bioRxiv - Pathology 2024Quote: ... Gradient gels were then transferred to 0.45-μm nitrocellulose membranes (VWR, #27376-991) for 1 hr at 75 V in ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad ...
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bioRxiv - Pathology 2024Quote: ... The gradient gels were transferred onto 0.45-μm nitrocellulose membranes (VWR, #27376-991) for 1 hour at 75 V using ice-cold 20% methanol in 1X Tris-glycine buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... the C18 membrane was activated using 20 μL LC-MS grade MeOH (VWR), cleaned by 20 μL 80% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Warm phosphate buffer (0.1 M; 21.7 mM NaH2PO4 [Chem Supply, #SA061-500G], 81 mM Na2HPO4 [VWR Chemicals, #102494C], pH 7.2) was perfused ...
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bioRxiv - Biophysics 2023Quote: ... Beads were resuspended in 500 μl of 0.1 M pH5 MES buffer (VWR, Radnor, PA, USA, Cat. No. E149-100G). 12.5 μl of the 0.2 g/ml EDC solution and 50 μl of the 1 mg/ml mPEG-NH2 solution were then added to the bead solution and incubated overnight at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 5μm consecutive sections to the sections used for Visium FFPE were placed onto Superfrost Plus microscope slides (#631-9483, VWR) and stored at 4°C until used for RNAScope and in situ sequencing ...
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bioRxiv - Immunology 2023Quote: ... After the 10 minutes the reaction were stopped with 100 μL 0,5 M sulphuric acid (VWR Chemicals, cat no: 30144.294). The plates were read at 450 nm on an EL808 Ultra Microplate Reader (BioTek Instruments)
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bioRxiv - Neuroscience 2024Quote: ... in 0.1 M phosphate buffer (80 mM Na2HPO4 and 19 mM NaH2PO4, pH 7.4) containing 0.003% glutaraldehyde (ref # 1875, VWR, Radnor, PA). Brains were removed and post-fixed overnight at 4°C in 4% PFA ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration in KGM was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). Cells were maintained at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). For all hTCEpi cell experiments ...
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bioRxiv - Bioengineering 2024Quote: ... NO sensing electrode was immersed with 25 µM NO in PBS (0.01 M, pH = 7.4) (VWR, catalog no. 392-0442). LSV peak was fitted by using linear baseline subtraction in the PSTrace software.
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bioRxiv - Bioengineering 2024Quote: ... CV was measured in 1 mM [Fe(CN)6]3- in 1 M KCl solution (VWR, catalog no. BDH9258-500G) at a scan rate of 200 mV/s.
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and filtered through a 0.22 µM polyethersulfone (PES) membrane filter (VWR) and stored in aliquots at −80 ...
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bioRxiv - Cell Biology 2020Quote: ... The cytosol fraction in the supernatant was concentrated using 10K Polyethersulfone (PES) membranes (VWR) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Membrane was washed in TBST and blocked in TBST + 5% milk (VWR # 10128-600) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Collected supernatants were then passed through a 5.0µm acrodisc versapor membrane syringe filter (VWR). The filtrate was then pelleted at 16,000xg for 2min ...
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bioRxiv - Microbiology 2021Quote: ... cultures were separated by membranes of different pore sizes in 6-well plates (VWR). Inserts with membrane pore size of 0.4 µm (VWR 10769-192 ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in blocking buffer consisting of 5% bovine serum albumin (BSA; VWR) dissolved in 1X Tris-buffered saline (TBS ...