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Citations for VWR :
1601 - 1650 of 1895 citations for 6H Dibenzo a g quinolizinium 5 8 13 13a tetrahydro 2 9 dihydroxy 3 10 dimethoxy 7 methyl 7S 13aS since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Cell Biology 2021Quote: ... decalcification solution for 14 days and then paraffin embedded for sectioning with a Leica RM2235 microtome at 5 μm per section to generate histology slides (VWR 631-1553). Sections were processed and stained with TRAP solution and counterstained with 0.2% methyl green solution (Bioenno Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Microbiology 2022Quote: ... containing 1.5 ml of brain–heart infusion broth ([BHI]; Becton Dickinson, Franklin Lakes, NJ) supplemented with 0.1% Tween 80 (VWR Chemicals, Solon, OH) and were incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biophysics 2023Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Cell Biology 2020Quote: ... dechorionated embryos were mounted on glue-coated coverslips (0.17mm, grade #1.5) and covered with Voltalef 10S oil (VWR). Movies of GFP::M6 accumulation were acquired using a 40x/1.3 NA oil immersion objective and the SP8 resonant scanner (line accumulation of 2 ...
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bioRxiv - Genomics 2019Quote: ... with 10 strokes using a loose pestle and filtered through a 100 µm nylon mesh (VWR #10199-658) followed by 20 strokes using a tight pestle and centrifuged for 1 minutes at 100g ...
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bioRxiv - Bioengineering 2021Quote: ... 10 grams of cranberry powder was mixed with 100 mL of absolute ethanol (≥99.8%, VWR chemicals, AnalaR Normapur) under magnetic stirring for 30 minutes at 24±1°C sealed with parafilm ...
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bioRxiv - Bioengineering 2020Quote: ... SI was rolled using the Swiss-rolling technique and incubated in 10% Neutral Buffered Formalin (VWR, 10790-714) for 24 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS; Heat Inactivated Seradigm Premium Grade Fetal Bovine Serum, VWR, Radnor, PA) and 1 mM sodium pyruvate (Corning Inc. ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: Cell line MDA MB 436 was propagated in DMEM media supplemented with 10% FBS (VWR Seradigm Life Science), 1% L-glutamine and 1% sodium pyruvate (Life Technology-Thermo-Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 30 minutes in permeabilization buffer (PB; 0.5% Bovine serum albumin (VWR International; 126575-10), 0.25% Fish skin gelatin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 60-minute blocking with donkey serum (1:10 diluted in 1% BSA; VWR, Pennsylvania, USA). Incubation with the primary antibody was performed overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Mice (10-12 weeks) were suspended by their tails with adhesive tape (VWR or Fisher, 25 mm wide) to a flat metal bar connected to a force transducer that measures transduced movement ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... H2O up to a total volume of 10 μl was combined in a sealed short thread vial (VWR). For reducing condition freshly prepared DTT was added to a final concentration of 2 mM ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μm ventricular sections were cut using a Leica cryostat and attached to SuperFrost Plus™ slides (VWR). Slides were frozen at -20prior to immunostaining.
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bioRxiv - Biochemistry 2020Quote: ... 10 mM tetraoctylammonium bromide (TOAB) and solvent B contained 30 mM TOAB dissolved in HPLC grade acetonitrile (VWR). The column was subsequently cleaned for 10 mins using solvent B (isocratic ...
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bioRxiv - Microbiology 2020Quote: ... Growth media were loaded into plastic 10 mL syringes (Codan) or glass vials (VWR, cat. no. 548-0154) and warmed to 37 °C at least 3 h prior to the start of each experiment.
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bioRxiv - Cell Biology 2021Quote: Embryoid bodies and monolayer cultures were fixed in 10% buffered formalin (Cat# CA71007-344, VWR, Radnor, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: α-Syn (1 mg/mL) was dissolved in 10 mM NaOH and sonicated (VWR® Symphony™ Ultrasonic Cleaner) for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... dechorionated embryos were mounted and lined up on a Menzel-Glaser coverslip with oil 10-S Voltalef (VWR) and covered with a membrane (YSI membrane kit) ...
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bioRxiv - Immunology 2023Quote: ... whole livers were harvested from euthanized mice and placed in 10% buffered formalin (VWR Cat. No. 16004-128) in 50 mL conical tubes ...
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bioRxiv - Cell Biology 2023Quote: ... temperature-mediated antigen retrieval was performed in citrate buffer (10 mM citric acid monohydrate (VWR, 20276.235, Pennsylvania, USA), Ph 6 ...
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bioRxiv - Biochemistry 2024Quote: ... and 10% glycerol with Cy3-Mono-Reactive Dye or Cy5-Mono-Reactive Dye overnight (VWR Catalog# 95017-379). The dye-labeled Cy3-RAD51 and Cy5-RAD51 was further purified as described above to remove any free dye in the protein prep ...
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bioRxiv - Neuroscience 2024Quote: ... mice were transcardially perfused with 1x PBS (137 mM NaCl, 2.7 mM KCl, and 10 mM PB, VWR) and then 4% PFA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Zoology 2019Quote: ... Agar-based assay plates were prepared by adding 70 μl of 2% Agar (Bacto Agar, VWR, Becton Dickinson Sparks, MD) and 70 μl of thiabendazole solution to each well ...
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bioRxiv - Bioengineering 2021Quote: ... An array of 2 mm holes was punched in the channel outlets using a biopsy punch (VWR, Cat# 21909-132). A hole was drilled on a plate lid (VWR ...
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Microbiology 2020Quote: A Ninjaflex 2 mm thick gasket was 3D printed onto a 50 mm × 75 mm × 1 mm glass slide (VWR) using a Makerbot 2× printer (Appendix I) ...
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bioRxiv - Bioengineering 2021Quote: ... well plates were washed two times with MilliQ H2O and then stained with 400 μL of 2% w/V alizarin red S (VWR) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Microbiology 2020Quote: ... Transfections were conducted by electroporation using a BioRad Gene Pulser Xcell at 1.5 kV and 25 µF with 2 mm electroporation cuvettes (VWR international) in Cytomix buffer (10 mM KH2PO4/K2HPO4 (pH 7.6) ...
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bioRxiv - Genetics 2020Quote: ... HEPES pH 7.8 and 0.00004% methylene blue in autoclaved reverse osmosis water) and mounted them on coverslip bridges (2 x 22 mm square glass coverslips (16004-094, VWR) glued together on either side of a 24 x 60 mm glass cover slip (12460S ...
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bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...