Labshake search
Citations for VWR :
1601 - 1650 of 1695 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
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bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover for 48 hours before passage into new 10 cm plates containing DMEM media supplemented with 2 mg/ml Geneticin sulfate (G418; VWR). Following 7 days of selection ...
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bioRxiv - Bioengineering 2021Quote: ... well plates were washed two times with MilliQ H2O and then stained with 400 μL of 2% w/V alizarin red S (VWR) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Microbiology 2020Quote: ... Transfections were conducted by electroporation using a BioRad Gene Pulser Xcell at 1.5 kV and 25 µF with 2 mm electroporation cuvettes (VWR international) in Cytomix buffer (10 mM KH2PO4/K2HPO4 (pH 7.6) ...
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bioRxiv - Genetics 2020Quote: ... HEPES pH 7.8 and 0.00004% methylene blue in autoclaved reverse osmosis water) and mounted them on coverslip bridges (2 x 22 mm square glass coverslips (16004-094, VWR) glued together on either side of a 24 x 60 mm glass cover slip (12460S ...
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bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine culture of E.coli for cloning purposes was performed in Miller’s LB Broth (Fisher BP1426-2) in 14mL Falcon tubes (VWR 60819-761) at 37°C on a rotating drum ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were transferred to a 2 mL tube pre-filled with 2.8 mm ceramic beads (VWR, Cat. No. 10158-612), flash frozen on dry ice ...
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bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
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bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: The brain and lung tissue were transferred to a cold room and subjected to homogenization using a 2 ml Dounce homogeniser (VWR, Tissue grinders ...
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bioRxiv - Biochemistry 2023Quote: ... were obtained from MilliporeSigma. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride (Cat. # K831, CAS: 51805-45-9) was purchased from VWR. Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
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bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
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bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µL of each was added to 2 mL cultures of attached cells in 6-well plates (#10861-554, VWR) containing 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
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bioRxiv - Synthetic Biology 2023Quote: Sender strains and receiver strains were inoculated in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in a 2 mL deep-well 96-well plate (VWR), covered with a “Breathe Easier” membrane (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were lysed in TRK buffer with 2% β-mEtOH and homogenized using a peqGOLD RNA Homogenizer Column (VWR). Following centrifugation at ≥12,000 x g for 2 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 50 µ/mL carbenicillin overnight at 37 °C in a 96-well 2 mL deep-well plate (VWR) shaking at 1100 rpms ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Leaf tissue was collected 7 days post infection, transferred to an extraction bag (Bioreba, Switzerland) and mixed with 5 ml of phosphate buffered saline (VWR, West Chester, PA), hand homogenized using a hand-held tissue homogenizer (Agdia ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Neuroscience 2023Quote: ... to a 50-mL Falcon tube on ice and homogenized at a speed of ∼1000 rpm for 5 sec to brake nuclei clumps with a handheld homogenizer (VWR® 200 Homogenizer). After homogenization ...
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bioRxiv - Bioengineering 2024Quote: ... Cellular debris was then cleared by centrifugation at 1,000 g for 5 minutes at 4°C and then further filtered through a 0.45 μm cellulose acetate filter (VWR cat. no. 28145-481). The cleared media was then concentrated with 100 kDa Amicon cutoff filter (Millipore Sigma cat ...
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bioRxiv - Biochemistry 2024Quote: ... The blots were incubated in 5% nonfat dry milk prepared in 1X Tris-buffered saline with Tween 20 (TBS-T, VWR International, #J63682.K2) for 30 min at room temperature to block nonspecific binding sites ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Microbiology 2020Quote: ... Pigment-expressing GBS was concentrated in 50 µL of PBS and a 2 µL droplet was aliquoted and sandwiched between two cover slides (48393-230, VWR International). Changes in pigment presence were determined by measuring the change in Raman peak amplitudes associated with conjugated double bonds located at the 1126 cm-1 and 1510 cm-1 before and after light treatment ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Immunology 2020Quote: ... and the filter was washed with sterile 1x phosphate buffer saline (PBS) supplemented with 2% v/v fetal bovine serum (FBS, VWR, USA). Cell density was determined through trypan blue exclusion ...
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bioRxiv - Biochemistry 2020Quote: ... The 168 h incubation samples were sonicated for 2 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). Circular dichroism (CD ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...