Labshake search
Citations for VWR :
1151 - 1200 of 1695 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... containing three different concentrations of EtOH (5%, 10%, 20%, prepared using 96% EtOH; VWR international #83804.360 diluted to the correct concentration ...
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bioRxiv - Neuroscience 2024Quote: ... the coverslip was perfused with ECS containing: 140 mM NaCl (VWR, #7647-14-5), 5 mM KCl (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Neutrophils were seeded in transwell inserts with a pore size of 5 µm (VWR). Sixty microliters of mouse serum from C57BL/6 mice were added to 600 µl of 10% FBS in DMEM at the basolateral surface of the transwell ...
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bioRxiv - Bioengineering 2024Quote: ... a stock solution of 5% (w/v) GelMA in PBS (VWR, Radnor, PA, USA) containing 0.2% lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP ...
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bioRxiv - Biochemistry 2024Quote: ... were transformed with the plasmid and grown in 5 ml of LB media (VWR) supplemented with 100 μg/ml carbenicillin (final concentration ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...
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bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.1% Fast green (Raymond A Lamb) (both in distilled water) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Biochemistry 2022Quote: ... Cells on D-poly-Lysine coated coverslips were then fixed with pre-warmed 3.7% formaldehyde (v/v; VWR, VW3408-1), permeabilized with ice-cold methanol ...
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Systems Biology 2022Quote: Samples were grown using SD-His+G418 agar and medium selecting for the duplicated chromosomes (6.7 g/L yeast nitrogen base without ammonium sulfate, Difco Cat#233520; 20 g/L glucose, Sigma Cat#Y0626; 1 g/L monosodium glutamate, VWR Cat#27872.298 ...
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bioRxiv - Immunology 2022Quote: Bone marrow isolation and cell staining was performed on ice in staining medium (SM; Hanks Balanced Salt Solution [HBSS 1x, Corning 1-022-CM] supplemented with heat inactivated fetal bovine serum [FBS, VWR 97068-05] to a final concentration of 2%) ...
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50 μL of 1% (w/v) sodium dodecyl sulfate (SDS, VWR International, Radnor, PA, USA). Colorimetric absorbance at 414 nm was measured using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Pathology 2021Quote: ... 10% glacial acetic acid) at a 20:1 ratio for 30-45 min and 10% neutral buffered formalin (VWR scientific) at a 10:1 ratio for 24 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue sections were rinsed of excess probes in 2X SSCT for 1 min and washed twice in 50% formamide (VWR) in 2X SSC at 37°C for 25 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS pads were placed on the sample and were covered with 20×20 mm No 1 cover glass slide (VWR). The open sides between two cover glass slides were sealed using VALAP solution (1:1:1 w/w Vaseline-Lanolin-Paraffin).
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Bioengineering 2023Quote: ... The product was purified by a dialysis bag with 1 K MWCO followed by filtration with VWR syringe filter (0.22 μm; 76479-010; VWR, PA). To load DOX to GQDs (e.g. ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...
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bioRxiv - Bioengineering 2024Quote: ... Oral rinses in the anatomical scaffolds were performed on day 3 and day 5 post-inoculation using Listerine® Antiseptic mouthwash original (brown color) diluted in PBS (1x) to 1% and filtered using a vacuum filtration system (0.2 µm) (VWR, Philadelphia). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Then it was used to seed a main culture (200 µl final volume of BMM with desired concentration of Ca2+ and/or Fe) at a ratio of 1:100 in 96 well white clear bottom plates (VWR). The plates were covered with lids treated with a mixture of 95% ethanol and 1% Triton solution to prevent condensation ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Neuroscience 2024Quote: ... the enriched PBMCs at the middle layer were carefully removed and washed twice in 1% BSA (VWR, 9048- 45-8) in DMEM (Corning ...
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The in-house polyacrylamide TBE gels (40 ml) for horizontal electrophoresis were produced by combining 6 ml 40% Acryl/Bis 29:1 (VWR), 4 ml of 5x TBE ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Biophysics 2023Quote: ... at a mass ratio of 1000:1 TO:PL and bath sonicated for 30 min (Ultrasonic Cleaner 96043-936; VWR Symphony) to form aLDs ...