Labshake search
Citations for VWR :
1451 - 1500 of 1752 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... at a thickness of 5-30 μm and mounted onto Superfrost Plus slides (VWR, 48311-703).
-
bioRxiv - Microbiology 2024Quote: ... pyogenes strains which were grown in BHI supplemented with 5% heat-inactivated fetal bovine serum (VWR). For in vivo imaging of bacterial infection in mice ...
-
bioRxiv - Microbiology 2024Quote: ... heated at 95 °C for 5 min and subjected to bead beating twice on high (VWR) for 1 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 5 mL of 50 mM ammonium bicarbonate (ABC) + 2 M urea buffer (VWR, M123-1KG). After washing ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and was subsequently propagated in a well of an unshaken flat-bottom polypropylene 96-well plate in one of three conditions: YPD (1% Bacto yeast extract (VWR #90000–726), 2% Bacto peptone (VWR #90000–368) ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we plated a 150 μL of 1:100 diluted saturated culture of a sensitive strain (YAN563) on a single-well (VWR #46600-638) methylene blue agar plate (20 g/L peptone ...
-
bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with chemiluminesence substrate (Perkin-Elmer: NEL105001EA) for 1 min and signal was developed on GE Amersham Hyperfilm ECL (VWR: 95017-661). Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... The films were rinsed in dH2O and covered with large coverslips (80 × 120 mm, thickness no.1, VWR International AS, Oslo, Norway). Imaging was performed with an epi-fluorescence microscope (Olympus BX51) ...
-
bioRxiv - Biophysics 2022Quote: ... the surface of the glass coverslip serving as chamber bottom was incubated with 1% (10 mg/ml) bovine serum albumin (BSA; VWR, Radnor, PA) in phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... each well was washed three times with warm PBS and then treated with 500ul PBS with 1% Triton X-100 (AMRESCO, VWR, Randor PA) for 5 minutes ...
-
bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck, Germany). For this purpose ...
-
bioRxiv - Plant Biology 2021Quote: ... and 1/5th of the lysate was added into 40 µL lysis buffer and sheared in a sonicator (VWR Aquasonic 250D, 35Khz) for 3 min ...
-
bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
-
bioRxiv - Neuroscience 2021Quote: ... After which cells were washed once with 1x PBS with 1% BSA before being filtered through 40um FlowMi Tip Strainers (VWR, 10032-802). Cells were then diluted with 1x PBS with 1% BSA and loaded onto the Chromium 10x Controller according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μl of each reaction were mixed with loading dye and analyzed on a 1% (w/v) agarose gel containing GelRed (VWR, Darmstadt, Germany). The remaining PCR mix was purified using a QIAquick PCR Purification Kit (Qiagen ...
-
bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
-
bioRxiv - Immunology 2021Quote: ... Whole blood cell lysates or lysates of PBMCs from IL-1-Ra-Ab positive patients were treated with alkaline phosphatase as previously described using FastAP thermosensitive alkaline phosphatase (Fermentas/VWR, Darmstadt, Germany).(40 ...
-
bioRxiv - Bioengineering 2020Quote: ... and particles were removed from PDMS chip and glass substrate (76 x 26 x 1 mm microscope slides, VWR International GmbH, Germany) via isopropanol washing and drying with pressurized air ...
-
bioRxiv - Cancer Biology 2021Quote: ... was achieved using an adapted protocol from Ly et al.22 FFPE TMAs were first washed twice for 1 minute in a xylene bath (mixture of isomers; ≥98.5%, AnalaR NORMAPUR®, VWR, Leicestershire, UK). Residual xylene was removed ...
-
bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
-
bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
-
bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
-
bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
-
bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
-
bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
-
bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
-
bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... pre-cultures for reporter-based promoter activity assays were performed at 30°C and 900 rpm in 1 ml medium in 2.2 ml square-shaped V-bottom 96-deep well plates (VWR, Radnor, US-PA) covered with a sterile breathable rayon film (VWR ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resin was equilibrated with wash buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) and bound proteins were eluted in wash buffer supplemented with 20 mM glutathione ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM dithiotreitol (DTT; [VWR Chemicals])) supplemented with protease inhibitor [cOmplete ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
-
bioRxiv - Developmental Biology 2024Quote: ... was conducted in 1x Towbin buffer with 0.1 % SDS for 30 minutes (25 mM Tris-base, 192 mM Glycine, 1 % SDS) using a PerfectBlue Semi-Dry Electroblotter Sedec M (VWR; 700-1220). Transfer was performed at a constant current of 10 mA for 90 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... and the wing was imaged at selected ages through a cover glass of thickness 1.5 (VWR Micro Cover Glass, selected 1 ounce, 18×18 mm, No. 1.5, VWR Cat No 48366-205).
-
bioRxiv - Evolutionary Biology 2021Quote: ... which we then incubated at 25°C for 4 days prior to staining with iodine (VWR iodine crystals) vapor (Forsburg and Rhind 2006).
-
bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was kept overnight in a solution of PBS 0.01 mM/formaldehyde 4% (v/v; CAT#20909.330, VWR) and then cut in 50 μm slices with a vibratome (VT1000 ...
-
bioRxiv - Neuroscience 2020Quote: ... Mice were then perfused with 30 mL of cold PBS and 4% paraformaldehyde (#100503-916, VWR, Radnor, PA) and were immediately decapitated to collect the brains ...
-
bioRxiv - Developmental Biology 2021Quote: ... Fetuses and brains were then stored long-term at 4°C in 0.002% Sodium Azide (VWR - Bie&Berntsen) in PBS.
-
The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were mounted on ∼1.4% w/v agar pads (in sterile ultrapure water) and a 0.13- to 0.17-mm glass coverslip (VWR) was placed on top ...
-
bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... Samples were fixed with 4% PFA for 15 min and incubated with 40 mM Alizarin Red S (VWR, US) for 30 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... reference slides were generated using 4-micron thick sections mounted on SuperFrost Plus slides (VWR International, Lutterworth, UK) and reviewed by the neuropathologist who performed the autopsy ...
-
bioRxiv - Developmental Biology 2024Quote: Fish were anesthetized using tricaine and initially fixed by immersion in a solution of 4% paraformaldehyde (VWR:15713) and 0.1M sodium-cacodylate (VWR:11653) ...
-
bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
-
bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...