Labshake search
Citations for VWR :
1301 - 1350 of 1752 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50 μL of 1% (w/v) sodium dodecyl sulfate (SDS, VWR International, Radnor, PA, USA). Colorimetric absorbance at 414 nm was measured using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The in-house polyacrylamide TBE gels (40 ml) for horizontal electrophoresis were produced by combining 6 ml 40% Acryl/Bis 29:1 (VWR), 4 ml of 5x TBE ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...
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bioRxiv - Neuroscience 2024Quote: ... the enriched PBMCs at the middle layer were carefully removed and washed twice in 1% BSA (VWR, 9048- 45-8) in DMEM (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Microbiology 2024Quote: ... Then it was used to seed a main culture (200 µl final volume of BMM with desired concentration of Ca2+ and/or Fe) at a ratio of 1:100 in 96 well white clear bottom plates (VWR). The plates were covered with lids treated with a mixture of 95% ethanol and 1% Triton solution to prevent condensation ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Bioengineering 2024Quote: ... Oral rinses in the anatomical scaffolds were performed on day 3 and day 5 post-inoculation using Listerine® Antiseptic mouthwash original (brown color) diluted in PBS (1x) to 1% and filtered using a vacuum filtration system (0.2 µm) (VWR, Philadelphia). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Molecular Biology 2023Quote: ... Tissue sections were rinsed of excess probes in 2X SSCT for 1 min and washed twice in 50% formamide (VWR) in 2X SSC at 37°C for 25 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS pads were placed on the sample and were covered with 20×20 mm No 1 cover glass slide (VWR). The open sides between two cover glass slides were sealed using VALAP solution (1:1:1 w/w Vaseline-Lanolin-Paraffin).
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Biophysics 2023Quote: ... at a mass ratio of 1000:1 TO:PL and bath sonicated for 30 min (Ultrasonic Cleaner 96043-936; VWR Symphony) to form aLDs ...
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bioRxiv - Developmental Biology 2023Quote: ... and then to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Bioengineering 2023Quote: ... The product was purified by a dialysis bag with 1 K MWCO followed by filtration with VWR syringe filter (0.22 μm; 76479-010; VWR, PA). To load DOX to GQDs (e.g. ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... The fixed cells were washed twice with cold flow staining buffer and blocked by 1% bovine serum albumin (BSA) (Fraction V, cat. 422371X, VWR) in PBS for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... to measure the radial expansion for each graft whereas thickness was measured on 1-2 mm length graft cross-sections imaged under phase contrast microscopy (VWR). Burst pressure was defined as the highest pressure recorded on the pressure monitor during the burst experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Microbiology 2024Quote: ... overnight cultures of each strain were back diluted 1:50 into 14 mL of pre-reduced mRCM in 100x15mm circular petri dishes (VWR) to optimize exposure to oxygen ...
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bioRxiv - Molecular Biology 2024Quote: ... (newly assigned) were used for amplification with a primer concentration of 1 µM and the help of a PCR Mastermix (VWR Life Science ...
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bioRxiv - Molecular Biology 2024Quote: ... polyallomer tubes were placed in an ice bucket and the top 1 mL (termed top fraction) was carefully removed with a pipettor and placed in a 2 mL microtube containing 1 mL Trizol (VWR). The remaining sucrose was carefully poured/discarded ...
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bioRxiv - Physiology 2024Quote: ... Muscles were immediately placed into warmed collagenase solution (2 mg/ml Type I collagenase [MB-122-0050, Rockland Immunochemicals, Limerick, PA, USA], 1% penicillin-streptomycin [Pen-Strep, 97063-708, VWR, Radnor ...
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bioRxiv - Bioengineering 2024Quote: ... We used a 1 : 10 ratio of tissue to deionized water and homogenized the tissue samples in a bead-mill homogenizer (VWR). We then diluted the tissue homogenates 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Microbiology 2020Quote: ... were cut with a cryostat and 3 sections of 20 µm were placed on each microscope glass slide (VWR). Sections were fixed with acetone for 10 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3-D spheroids were generated by seeding 2.0E6 cells per well of a 6-well cell repellent plate (VWR) in a final volume of 4.0 mL complete media and placed on an orbital shaker at 95 RPM for 24 hours ...
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bioRxiv - Synthetic Biology 2020Quote: 3 biological replicates of each isolate were grown overnight in 96-deep-well plates (VWR International, cat #10755-248) in LB media ...
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bioRxiv - Biochemistry 2021Quote: Custom Eppendorf tubes for extract recovery were created by cutting approximately 3 mm from the bottom of a standard 0.5 mL Eppendorf tube (VWR). The tubes were then soaked in 70% ethanol to remove small molecule contaminants ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...