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Citations for VWR :
1351 - 1400 of 1469 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... After the 10 minutes the reaction were stopped with 100 μL 0,5 M sulphuric acid (VWR Chemicals, cat no: 30144.294). The plates were read at 450 nm on an EL808 Ultra Microplate Reader (BioTek Instruments)
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bioRxiv - Bioengineering 2023Quote: ... the pigs were euthanized with intravenous barbiturate and the graft was excised and flushed with 300 mL PBS followed by 300 mL 10% neutral buffered formalin (NBF, VWR). If the kidneys could not be fully flushed due to thrombosis ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were cut at a thickness of 10 μm using a Leica CM1950 cryostat and placed onto SuperFrost Plus slides (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Embedded tissue blocks were sectioned on a cryostat in slices 10 to 20 micrometers thick and attached to Superfrost Plus microscope slides (631-0108, VWR). The sections can be stored at −80 indefinitely ...
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bioRxiv - Pathology 2024Quote: ... Reverse transcription was performed for 30 min at 42 °C using dNTP Mix (10 mM each, VWR, Radnor, PA, USA) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The resulting monolayer of cells was isolated and washed using RPMI1640 (Stemcell, #36750) supplemented with 10% FBS (VWR, #97068-085), Pen/Strep (Thermo ...
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bioRxiv - Synthetic Biology 2024Quote: The variant BUD-ELMs were grown in standard conditions and then collected into a 1.5 ml Eppendorf tube and spun for 10 s at 3200 rcf using a mini centrifuge (VWR - C0803). Then the supernatant was removed ...
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bioRxiv - Cancer Biology 2024Quote: ... epithelial-stromal composites were incubated for 30 min in permeabilisation buffer (PB1; 0.5 % bovine serum albumin (VWR International, 126575-10), 0.25 % fish-skin gelatin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... They were treated to a 1:1 mixture of 100% ethanol and methyl salicylate for 10 minutes before clearing and storage in methyl salicylate (VWR Chemicals).
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bioRxiv - Cell Biology 2020Quote: ... which was mixed thoroughly and allowed to stand for 10 minutes prior to filtration through a 0.2μM vacuum filter unit (VWR, Cat # 10040-460) to remove particulates ...
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was suspended in 1 ml PBS at room temperature (POPC) or 55 °C (DPPC) and then sonicated for 10 minutes in an ultrasound bath (USC500TH, VWR, England) at the same temperature to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
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bioRxiv - Bioengineering 2020Quote: ... of BIO-133 or 10% PEG-DA (ESIBIO GS705) on a #1.5 cover glass (24 mm x 50 mm, VWR, 48393-241). The droplet was positioned between two 100 μm spacers (Precision Brand ...
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bioRxiv - Microbiology 2020Quote: All strains were cultured for 10 hours with aeration at 37°C in brain heart infusion (BHI; VWR, Radnor, PA, USA) medium ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Cell Biology 2020Quote: Mid third-instar larval (L3) brains and imaginal discs were dissected in fresh Phosphate-Buffered Saline 10X (PBS, VWR #L182-10) and fixed for 30 minutes (min ...
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bioRxiv - Synthetic Biology 2022Quote: ... An approximate volume of 100-200 µL of BUD-ELMs were collected into a 1.5 mL centrifuge tube and spun for 10 sec at 3200 rcf with a mini centrifuge (VWR®, C0803). This allowed for the material to collect at the bottom as an homogeneous paste ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... The spacers supported the top 24×40mm #1 (0.13-0.16mm) coverslip (Knittel Glass) placed over the pupae and covered with a fine layer of mineral oil 10 S (VWR International). The top coverslip was glued to the spacers using nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Neuroscience 2019Quote: ... All odorant exposures in this study consisted of 10 μL of stimulus spotted onto a cut blotting pad (VWR 28298-014) placed inside an odor cassette (Tissue-Tek 0006772-01) ...
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bioRxiv - Immunology 2019Quote: ... The tissues were mashed using the plunger of a 10 ml syringe on a 40 micron nylon filter (VWR; 10199-654). The filters were washed with MACS buffer (DPBS with 2% FBS ...
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bioRxiv - Microbiology 2020Quote: ... then boiled for 10 minutes or placed in an uncovered polystyrene petri dish (60 mm x 15 mm, VWR, Radnor, PA) for exposure ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Developmental Biology 2021Quote: Wandering third-instar larval (L3) brains and imaginal discs were dissected in fresh Phosphate-Buffered Saline 10X (PBS, VWR #L182-10) and fixed for 30 minutes (min ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Microbiology 2020Quote: ... and sonicated for 15 min in a water bath before transfer to a 10 kDa molecular weight cut-off filter (VWR Scientific). After 15 min of centrifugation at 14,000 × g (20°C) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in DMEM (PAN-Biotech GmbH, Aidenbach, Germany) supplemented with 10% (v/v) fetal bovine serum (VWR International GmbH) and 0.2% penicillin-streptomycin (PAN-Biotech) ...
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bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
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bioRxiv - Microbiology 2022Quote: ... The composition of mobile phase A was 10 mM ammonium acetate at pH 9 in 90% acetonitrile LC–MC grade (VWR Chemicals) and 10% ultra-pure water from Direct-Q 3 UV Water Purification System with LC-Pak Polisher (Merck KGaA ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were immersed in a mixture of 100% ethanol and methyl salicylate (1:1, 10 minutes) before being stored in methyl salicylate (VWR Chemicals). Some slices were stored in a cryoprotective solution containing glycerol (20% ...
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bioRxiv - Immunology 2023Quote: ... Core temperature was measured at 10-minute intervals for 40 minutes post- challenge using a rectal probe (VWR International, 23226-656). Hemoconcentration was measured using the HemataSTAT II centrifugation device (VWR International ...
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bioRxiv - Genomics 2023Quote: SDS treated cells were resuspended in 400 μl of RT mix (final concentration of 1x RT buffer, 500 mM dNTP, 10 mM Biotinylated RT primers, 7.5% PEG 6000 (VWR, 101443-484), 0.4U/ml SUPERase•In™ RNase Inhibitor ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample (10 μL) was loaded and separated on an SDS-PAGE followed by Coomassie G-250 staining (VWR, Cat. # 0615) to visualize the overexpression of the desired fusion protein.
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then washed with 25 mL of ice cold STM buffer (pH = 7.5, 270 mM sucrose, Millipore Sigma, 1.07651.5000, 10 mM Tris HCl, Alfa Aesar, J67233, 1 mM MgCl2, VWR, E525-100ML) and spun down again at 4°C for 5 minutes at 1500 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... TSC feeder-dependent cells were cultured on mitomycin-treated MEFs at a 40-60% density in RPMI1640 (VWR, 10-040-CV) supplemented with 20% fetal bovine serum (GIBCO ...
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bioRxiv - Physiology 2023Quote: ... Tissue samples were cut serially sectioned at 30μm using the Leica cryostat (CM1860 UV) and placed across 10 SuperFrost microscope slides (VWR International; UK).
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bioRxiv - Immunology 2023Quote: ... Plaque assays were developed at 5dpi by removal of overlay media and staining/fixation using 10% neutral buffered formalin (VWR, #89370) and 0.25% crystal violet (VWR ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 – 20 mosquitoes were sorted into a behavioral container made from a 32-ounce HDPE plastic cup (VWR #89009-668). The behavioral container was prepared by cutting a 10 cm hole in the lid with a razor blade and covering the cup with a 20 cm x 20 cm piece of white 0.8 mm polyester mosquito netting (American Home & Habit Inc ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were grown at 22°C in Schneider’s Drosophila medium supplemented with 10% Fetal Bovine Serum (FBS) (VWR, Radnor, PA) and 0.5% Penicillin/streptomycin (Sigma-Aldrich) ...