Labshake search
Citations for VWR :
1201 - 1250 of 1469 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were re-dissolved in 10 μl 1% formic acid (VWR Ltd., Radnor, PA, USA) and were separated in a 180 min water/acetonitrile gradient using an Easy nLC 1200 nano UPLC (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Tissues were harvested following sacrifice via CO2 inhalation and fixed in 10 % formalin (VWR, Radnor, PA) for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: The adipose tissue was immediately perfused with PBS and fixed with 10% neutral-buffered formalin (VWR). Adipose tissues were then transferred to 70% ethanol ...
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bioRxiv - Immunology 2023Quote: ... The cells were fixed by dipping 10 times in pre-cooled ice-cold acetone (VWR, #20066.296) in a glass tray ...
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bioRxiv - Microbiology 2024Quote: The left lung from each mouse was collected in buffered 10% formalin (Q-Path VWR Chemicals), included in paraffin ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was extracted from the skull and post-fixed in 10% formalin (VWR, Radnor, PA) for at least 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... DMEM and RPMI-1640 media were supplemented with 10% heat-inactivated fetal bovine serum (FBS, VWR). U937 cells (a kind gift from Dr ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Developmental Biology 2024Quote: ... we generated a sterile circuit by pumping 50 mL of medium from a 50 mL conical tube reservoir using a Masterflex Ismatec Reglo Independent Channel Control Peristaltic Pump (MFLX78001-80, 4-channel, 8-roller; Avantor/VWR, Radnor, PA, USA) fitted with 1.52 mm inner diameter 3-stop tubing (95625-36 ...
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bioRxiv - Bioengineering 2024Quote: ... All dilutions of targets were performed in 4× PBS with 2 μM dT10 in GeneMate low-adhesion 1.7-mL microcentrifuge tubes (VWR, Cat No. 490003-230). PCR tubes containing target mixtures were then heated in a thermocycler to 73 °C for 3 min ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells transfected with vector-only control or A30P αSyn with and without AcNPs were fixed using a 4% paraformaldehyde (VWR, Cat# ALFAJ61899.AK) in PBS solution for 20 min and washed with PBS and 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35150™) was cultured overnight in 3 mL of brain heart infusion (BHI) growth medium (VWR International, LLC, 95021-488) using a shaker-incubator at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 10% FBS)) and filtered through a 100 uM cell strainer (cat no. 21008-950, VWR, Radnor PA). Cell density was quantified using a Countess II Automated Cell Counter (cat no ...
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bioRxiv - Cell Biology 2020Quote: Dechorionated embryos were immobilised with glue on a coverslip and covered with Oil 10-S Voltalef (VWR). To visualise tracheal Shot in vivo ...
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bioRxiv - Cell Biology 2020Quote: ... Thin sections of tissue (10 ⎧m) were mounted in 24-well glass bottom plates (VWR 82050-898) coated with a 1:50 dilution of APTES (Sigma 440140) ...
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bioRxiv - Molecular Biology 2020Quote: ... was purchased from American Type Culture Collection (ATCC) and was maintained in RPMI 1640 Medium (ATCC modification) with 10% FBS (VWR Life Science Seradigm) and 0.01% Penicillin-Streptomycin (10,000 U/mL) ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila melanogaster S2R+ cells were maintained in Schneider’s Insect Medium supplemented with 10% heat inactivated FBS (VWR) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... was purchased from American Type Culture Collection (ATCC) and was maintained in RPMI 1640 Medium (ATCC modification) with 10% FBS (VWR Life Science Seradigm) and 0.01% Penicillin-Streptomycin (10,000 U/mL ...
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bioRxiv - Cell Biology 2019Quote: ... Drosophila egg chambers were dissected and mounted in the gas-permeable oil 10S VOLTALEF® (VWR chemicals) for live imaging.
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bioRxiv - Neuroscience 2020Quote: ... Gentle irrigation with phosphate-buffered saline (137 mM NaCl, 27 mM KCl, 10 mM phosphate buffer, VWR) was used to clear debris at regular intervals ...
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bioRxiv - Bioengineering 2021Quote: ... The resultant product was dialysed in a 10 kDa MWCO dialysis tube (Spectra/Por, regenerated cellulose, VWR) for 3 d in 100 mM ...
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bioRxiv - Immunology 2020Quote: ... counted and kept frozen in aliquots at −150°C in FBS with 10 % dimethyl sulfoxide (DMSO, VWR) for future experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-30μg samples were prepared with a 6x reducing sample buffer containing β-mercaptoethanol (VWR, M131). Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic ...
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bioRxiv - Microbiology 2023Quote: ... After 72 h media were aspirated and cells were fixed in 10% neutral buffered formalin (NBF) (VWR) for 20 minutes (min) ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% fetal bovine serum (Avantor, Radnor Township, PA) and 1% penicillin/streptomycin (VWR, Radnor, PA). Cultures were grown in an incubator set at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:12 series of sections were quenched for 5 min using 10% H2O2 (VWR, West Sussex, UK) and methanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections measuring 10 μm containing the LC were then mounted on SuperFrost Plus slides (VWR, The Netherlands) and allowed to dry at −20 °C for 2 h ...
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bioRxiv - Physiology 2024Quote: ... 10 % Nipagin (SLS, FLY1136) in ethanol and an acid mixture comprised of 4.15 % phosphoric acid (VWR, 20624.262) and 41.8 % propionic acid (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Larvae were cut perpendicular to the caudal notochord boundary using a surgical blade (Feather No. 10, VWR). Burn injury was performed on anesthetized larvae in a 60 mm tissue culture-treated dish containing 1x Tricaine with E3 ...
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bioRxiv - Neuroscience 2024Quote: ... the overlay media was removed and cells were fixed/stained using 10% neutral buffered formalin (VWR #89370) and 0.25% crystal violet (VWR #0528 ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... One-day-old biofilms were grown on the surface of a 4-well Permanex slide (Lab-Tek® Chamber Slide™ System, VWR, Debrecen, Hungary). The preformed biofilms were washed three times with sterile physiological saline ...
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bioRxiv - Cell Biology 2020Quote: ... dechorionated embryos were mounted on glue-coated coverslips (0.17mm, grade #1.5) and covered with Voltalef 10S oil (VWR). Movies of GFP::M6 accumulation were acquired using a 40x/1.3 NA oil immersion objective and the SP8 resonant scanner (line accumulation of 2 ...
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bioRxiv - Genomics 2019Quote: ... with 10 strokes using a loose pestle and filtered through a 100 µm nylon mesh (VWR #10199-658) followed by 20 strokes using a tight pestle and centrifuged for 1 minutes at 100g ...
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bioRxiv - Bioengineering 2021Quote: ... 10 grams of cranberry powder was mixed with 100 mL of absolute ethanol (≥99.8%, VWR chemicals, AnalaR Normapur) under magnetic stirring for 30 minutes at 24±1°C sealed with parafilm ...
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bioRxiv - Bioengineering 2020Quote: ... SI was rolled using the Swiss-rolling technique and incubated in 10% Neutral Buffered Formalin (VWR, 10790-714) for 24 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS; Heat Inactivated Seradigm Premium Grade Fetal Bovine Serum, VWR, Radnor, PA) and 1 mM sodium pyruvate (Corning Inc. ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µl cells were mounted onto microscope slides containing a thin layer of 1 % agarose in water (supplemented with 10 % medium, where appropriate) and covered with 0.13-0.17 mm coverslips (VWR). Nucleoids were visualised using 1 µg/ml DAPI (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: Cell line MDA MB 436 was propagated in DMEM media supplemented with 10% FBS (VWR Seradigm Life Science), 1% L-glutamine and 1% sodium pyruvate (Life Technology-Thermo-Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated for 30 minutes in permeabilization buffer (PB; 0.5% Bovine serum albumin (VWR International; 126575-10), 0.25% Fish skin gelatin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 60-minute blocking with donkey serum (1:10 diluted in 1% BSA; VWR, Pennsylvania, USA). Incubation with the primary antibody was performed overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Mice (10-12 weeks) were suspended by their tails with adhesive tape (VWR or Fisher, 25 mm wide) to a flat metal bar connected to a force transducer that measures transduced movement ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... H2O up to a total volume of 10 μl was combined in a sealed short thread vial (VWR). For reducing condition freshly prepared DTT was added to a final concentration of 2 mM ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...