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Citations for VWR :
1151 - 1200 of 1285 citations for 8 DECYLOXYPYRENE 1 3 6 TRISULFONIC ACID TRISODIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... devices were treated in oxygen plasma and covalently bound onto borosilicate cover glass slides (round, 50 mm diameter, thickness No. 1, VWR). All microflow experiments were conducted in single microchannels with dimensions of either of μm in width and 100 μm in height or a width of 50 μm and a width of 200 μm.
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bioRxiv - Genomics 2019Quote: ... The mix was filtered through a set of sieves (1 mm/500 μm/38 μm × 200 mm diameter, (VWR, Sweden). The content of the last sieve was transferred into a falcon tube containing 20 ml of 60% sucrose solution ...
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50 μL of 1% (w/v) sodium dodecyl sulfate (SDS, VWR International, Radnor, PA, USA). Colorimetric absorbance at 414 nm was measured using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Microbiology 2024Quote: ... Then it was used to seed a main culture (200 µl final volume of BMM with desired concentration of Ca2+ and/or Fe) at a ratio of 1:100 in 96 well white clear bottom plates (VWR). The plates were covered with lids treated with a mixture of 95% ethanol and 1% Triton solution to prevent condensation ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Bioengineering 2024Quote: ... Oral rinses in the anatomical scaffolds were performed on day 3 and day 5 post-inoculation using Listerine® Antiseptic mouthwash original (brown color) diluted in PBS (1x) to 1% and filtered using a vacuum filtration system (0.2 µm) (VWR, Philadelphia). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Molecular Biology 2023Quote: ... Tissue sections were rinsed of excess probes in 2X SSCT for 1 min and washed twice in 50% formamide (VWR) in 2X SSC at 37°C for 25 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS pads were placed on the sample and were covered with 20×20 mm No 1 cover glass slide (VWR). The open sides between two cover glass slides were sealed using VALAP solution (1:1:1 w/w Vaseline-Lanolin-Paraffin).
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Biophysics 2023Quote: ... at a mass ratio of 1000:1 TO:PL and bath sonicated for 30 min (Ultrasonic Cleaner 96043-936; VWR Symphony) to form aLDs ...
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bioRxiv - Developmental Biology 2023Quote: ... and then to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Bioengineering 2023Quote: ... The product was purified by a dialysis bag with 1 K MWCO followed by filtration with VWR syringe filter (0.22 μm; 76479-010; VWR, PA). To load DOX to GQDs (e.g. ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... The fixed cells were washed twice with cold flow staining buffer and blocked by 1% bovine serum albumin (BSA) (Fraction V, cat. 422371X, VWR) in PBS for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Molecular Biology 2021Quote: We prepared Aβ1-42 oligomers (oAβ) and fibrils (fAβ) following Evans (2006) and Ryan et al (2010) using siliconized polypropylene tubes (VWR #4165SL). We used Aβ1-42 to test the effects of the hybrid protein because it is more predominant in AD plaques (Nirmalraj et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...