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Citations for VWR :
1101 - 1150 of 1285 citations for 8 DECYLOXYPYRENE 1 3 6 TRISULFONIC ACID TRISODIUM SALT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Microbiology 2023Quote: ... cells were scraped into 1 mL fresh KA medium using disposable cell scrapers with 20 mm blade width (VWR). Cells were pelleted with the respective supernatants discarded and then flash frozen in a dry ice-ethanol bath to preserve RNA integrity and stored at −80°C ...
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 monocytes were differentiated into macrophages by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA, VWR) for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were diluted in 1 mL PBS and they or BALF were shaken in a glass vial (VWR) with 2 mL chloroform and 1 mL methanol containing internal standards (13C16-palmitic acid ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Cell Biology 2024Quote: For indirect immunofluorescence experiments cells were trypsinized and transferred either to Ø 18 mm cover glasses (No. 1; VWR) in 12-well tissue culture plates (TPP ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted fractions were pooled and further purified on a 1 ml HiTrap FF Q column (VWR 17-5053-01) using an AKTA FPLC system (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... myofibril pellets were resuspended in 300 μL of ice-cold wash buffer (Buffer 2: 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 1 mM DTT; all chemicals from VWR), and tubes were centrifuged at 3,000 g for 10 minutes at 4ºC ...
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bioRxiv - Neuroscience 2024Quote: ... S3: the lysate was extracted with an equal volume of phenol:chloroform:iso-amyl alcohol (25:24:1) (VWR, 97064-692), and the upper aqueous phase was then precipitated from ethanol.
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bioRxiv - Biochemistry 2024Quote: ... 500 µL of the upper phase was transferred in a new tube containing 1 mL isopropanol (VWR, 84881-320) and 2 µL f 15 mg/mL Glycoblue (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... adjusted for 1 hour in a 50:50 solution of 30% sucrose solution: Optimal Cutting Temperature (OCT) compound (VWR), followed by 1 hour in 100% OCT and snap-frozen in OCT using dry ice ...
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bioRxiv - Synthetic Biology 2024Quote: HEK293T cells were seeded 1 day before transfection at 100,000 cells/well in 24-well plates (Multiwell cell culture plates, VWR) for the transient transfections ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was immediately and gently mixed with 1 ml NCM medium containing 0.38 M mannitol and recovered in a round bottom tube (VWR) at 37 °C on a rotary shaker (120 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 20% glycerol, 1 mM DTT, 50 mM spermidine; all chemicals from VWR), and stored at -80°C for analyses described below.
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Neuroscience 2019Quote: ... was made with a dental drill (Osada) for injection into the target (see Table 1) using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Immunology 2019Quote: ... cells were plated at a density of 1 x 105 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...
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bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Biochemistry 2022Quote: ... Cells on D-poly-Lysine coated coverslips were then fixed with pre-warmed 3.7% formaldehyde (v/v; VWR, VW3408-1), permeabilized with ice-cold methanol ...
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: A Ninjaflex 2 mm thick gasket was 3D printed onto a 50 mm × 75 mm × 1 mm glass slide (VWR) using a Makerbot 2× printer (Appendix I) ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Systems Biology 2022Quote: Samples were grown using SD-His+G418 agar and medium selecting for the duplicated chromosomes (6.7 g/L yeast nitrogen base without ammonium sulfate, Difco Cat#233520; 20 g/L glucose, Sigma Cat#Y0626; 1 g/L monosodium glutamate, VWR Cat#27872.298 ...
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bioRxiv - Microbiology 2019Quote: ... excess oil was removed by pipetting and water-in-oil emulsions were broken by adding an equal amount of 1H,1H,2H,2H-Perfluoro-1-octanol (PFO, VWR) and briefly vortexed ...
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bioRxiv - Microbiology 2019Quote: ... ground with 1 mL of phosphate buffered saline using the sterile 15 mL conical tissue grinder (VWR™, Radnor, PA). Samples were spun at 5,000xg ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...