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Citations for VWR :
951 - 1000 of 1098 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were wet-mounted using 200 μl of 10% glycerol in PBS and 24 x 50mm glass cover slips (Cat # 48393-081, VWR). Following imaging ...
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bioRxiv - Biophysics 2019Quote: ... The reactions were quenched by the addition of SDS-PAGE loading buffer and analyzed by 10% Tricine-SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (VWR).
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bioRxiv - Bioengineering 2020Quote: ... The norbornene-functionalized alginate product was purified by dialysis in a 10 kDa MWCO dialysis tube (Spectra/Por, regenerated cellulose, VWR) in 100 mM ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% heat inactivated Fetal Bovine Serum (Thermo Fisher Scientific, Asheville, NC, Gibco or VWR Life Science, Randor, PA), 1x Penicillin Streptomycin Solution (Corning Incorporated ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted onto slides with nPG Antifade mounting medium (nPG stock : Glycerol : 10X PBS in the ratio 1:90:10) and edges were sealed with nail polish (VWR).
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bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
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bioRxiv - Microbiology 2019Quote: ... aeruginosa infected EVPL tissue pieces and uninfected control tissue were fixed in 10% (v/v) neutral buffered formalin (VWR Chemicals). The tissue samples were sent to the University of Manchester’s Histology Core Facility for paraffin wax embedding ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... cecal contents from specific-pathogen free (SPF) mice were resuspended in 10% weight/volume (w/v) Bacto Brain Heart Infusion (BHI) (VWR) supplemented with 0.05% w/v L-cysteine (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: Paraffin embedded brain sections were deparaffinized for 45 min at 60°C in the oven and 2x 10 min in Xylene (VWR). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: ... the inactivating solution was replaced by pre-warmed plating medium (10% horse serum (#S900-500, VWR International GmbH, Darmstadt, Germany), 1.8 mM glutamine ...
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bioRxiv - Cell Biology 2022Quote: ... HAF were seeded onto Ø 10 or 16 mm borosilicate glass cover glasses (631-0170 and 631-0151, VWR, Germany). After transfer into 12- or 24-well plates (665102 and 662160 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2024Quote: ... Reverse transcription was performed for 30 min at 42 °C using dNTP Mix (10 mM each, VWR, Radnor, PA, USA) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Washing was carried out three times for 10 min at room temperature with TBS + 0.1% Tween 20 (VWR, United States) and the membrane was incubated with appropriate fluorescent secondary antibodies (listed in Supplementary Table S1 ...
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bioRxiv - Neuroscience 2024Quote: ... CSF samples were diluted 10-fold in 0.02 µm filtered PBS and added to the glass cover slip (VWR, 6310135) and incubated for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Biophysics 2023Quote: ... All experiments were performed on cells grown in a double layer in 35 mm x 10 mm plastic dishes (#627160, Cellstar) containing 25 mm coverslips (#631-1072, VWR). Both the plastic dishes and the glass coverslips were coated with ornithine/laminin ...
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bioRxiv - Immunology 2023Quote: ... After the 10 minutes the reaction were stopped with 100 μL 0,5 M sulphuric acid (VWR Chemicals, cat no: 30144.294). The plates were read at 450 nm on an EL808 Ultra Microplate Reader (BioTek Instruments)
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bioRxiv - Bioengineering 2023Quote: ... the pigs were euthanized with intravenous barbiturate and the graft was excised and flushed with 300 mL PBS followed by 300 mL 10% neutral buffered formalin (NBF, VWR). If the kidneys could not be fully flushed due to thrombosis ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... Embedded tissue blocks were sectioned on a cryostat in slices 10 to 20 micrometers thick and attached to Superfrost Plus microscope slides (631-0108, VWR). The sections can be stored at −80 indefinitely ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were cut at a thickness of 10 μm using a Leica CM1950 cryostat and placed onto SuperFrost Plus slides (VWR).
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bioRxiv - Immunology 2023Quote: ... Cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Immunology 2023Quote: ... cells were plated at a density of 5 x 104 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Synthetic Biology 2024Quote: The variant BUD-ELMs were grown in standard conditions and then collected into a 1.5 ml Eppendorf tube and spun for 10 s at 3200 rcf using a mini centrifuge (VWR - C0803). Then the supernatant was removed ...
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bioRxiv - Cancer Biology 2024Quote: ... epithelial-stromal composites were incubated for 30 min in permeabilisation buffer (PB1; 0.5 % bovine serum albumin (VWR International, 126575-10), 0.25 % fish-skin gelatin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... We mixed 60 µl of the lysate with 400 µL of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) and loaded the mixture onto a 10 kDa 500 µL filter unit (VWR International) and centrifuged at 14,000 x g for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Microbiology 2024Quote: ... The resulting monolayer of cells was isolated and washed using RPMI1640 (Stemcell, #36750) supplemented with 10% FBS (VWR, #97068-085), Pen/Strep (Thermo ...
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bioRxiv - Neuroscience 2021Quote: ... They were treated to a 1:1 mixture of 100% ethanol and methyl salicylate for 10 minutes before clearing and storage in methyl salicylate (VWR Chemicals).
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bioRxiv - Cell Biology 2020Quote: ... which was mixed thoroughly and allowed to stand for 10 minutes prior to filtration through a 0.2μM vacuum filter unit (VWR, Cat # 10040-460) to remove particulates ...
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was suspended in 1 ml PBS at room temperature (POPC) or 55 °C (DPPC) and then sonicated for 10 minutes in an ultrasound bath (USC500TH, VWR, England) at the same temperature to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
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bioRxiv - Bioengineering 2020Quote: ... of BIO-133 or 10% PEG-DA (ESIBIO GS705) on a #1.5 cover glass (24 mm x 50 mm, VWR, 48393-241). The droplet was positioned between two 100 μm spacers (Precision Brand ...
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bioRxiv - Microbiology 2020Quote: All strains were cultured for 10 hours with aeration at 37°C in brain heart infusion (BHI; VWR, Radnor, PA, USA) medium ...
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bioRxiv - Microbiology 2020Quote: ... Samples for peak isolation were separated on a Vydac Semi-preparative C18 column (218TP510, 5 μm, 300 Å, 10 mm × 250 mm, VWR International B.V ...
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bioRxiv - Cell Biology 2020Quote: Mid third-instar larval (L3) brains and imaginal discs were dissected in fresh Phosphate-Buffered Saline 10X (PBS, VWR #L182-10) and fixed for 30 minutes (min ...
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bioRxiv - Synthetic Biology 2022Quote: ... An approximate volume of 100-200 µL of BUD-ELMs were collected into a 1.5 mL centrifuge tube and spun for 10 sec at 3200 rcf with a mini centrifuge (VWR®, C0803). This allowed for the material to collect at the bottom as an homogeneous paste ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... The spacers supported the top 24×40mm #1 (0.13-0.16mm) coverslip (Knittel Glass) placed over the pupae and covered with a fine layer of mineral oil 10 S (VWR International). The top coverslip was glued to the spacers using nail polish ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Neuroscience 2019Quote: ... All odorant exposures in this study consisted of 10 μL of stimulus spotted onto a cut blotting pad (VWR 28298-014) placed inside an odor cassette (Tissue-Tek 0006772-01) ...