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Citations for VWR :
51 - 100 of 197 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Physiology 2024Quote: ... The protein marker peqGOLD IV (ref: 27-2110, VWR) was used to quantify protein size ...
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bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a 0.45 µm nitrocellulose membrane (VWR), by electroblot ...
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bioRxiv - Immunology 2024Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
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bioRxiv - Biophysics 2024Quote: ... the protein was eluted with 20 mM reduced glutathione (VWR) in the binding buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody (donkey anti-rabbit IgG polyclonal antibody [horseradish peroxidase]; NA934) was from VWR International ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody (donkey anti-rabbit IgG polyclonal antibody [horseradish peroxidase]; NA934) was from VWR International ...
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bioRxiv - Microbiology 2024Quote: ... aureus polyclonal antibody or rabbit anti-CD68 polyclonal antibody (Cat. # BOSSBS-0649R, VWR, Radnor, PA, US) diluted 1:400 or 1:200 in 3% BSA in PBS respectively ...
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bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
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bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were sterilized by filtration (0.45 μm PES membrane filters, VWR).
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bioRxiv - Microbiology 2024Quote: Chemicals used for protein production and characterisation were purchased from VWR and used as received unless otherwise stated ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were transferred to nitrocellulose membranes (VWR, cat# 28298-020) which were blocked with 5% non-fat milk in Tris-Buffered Saline with Tween 20 (TBST) ...
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bioRxiv - Genomics 2020Quote: ... and Avadin-FITC antibodies (VWR CAP21221) in 10 μg/ml BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Neuroscience 2024Quote: ... or RNA and protein extraction (18 mm cover glasses [631-0153; VWR] in 12 well plates [142475 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein induction was carried out in bacteria grown in Terrific Broth (VWR) medium with 1mM IPTG (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibody (goat-anti-mouse-Cy3: VWR-95040-042 or goat-anti-rabbit-cy5 or Donkey anti-chicken-488 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... anti-OSR1 monoclonal antibody (VWR, 10624-616), anti-WNK1 antibody (Cell Signaling ...
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bioRxiv - Biochemistry 2024Quote: ... anti-OSR1 monoclonal antibody (VWR, 10624-616), anti-WNK1 antibody (Cell Signaling ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2024Quote: ... primary antibodies followed by a goat anti-rabbit IgG secondary antibody conjugated to HRP (VWR, cat. no. 102645-182). Following incubation with enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in the pellet were solubilized by sonication (incubation in Ultrasonic Cleaner bath, VWR) for 10 min followed by incubation for 60 min at 4 °C under rotation ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Genetics 2024Quote: ... then protein was lysed and harvested in 1X RIPA lysis buffer (VWR Life Science) supplemented with 1X cOmpleteTM Mini EDTA-free protease inhibitor cocktail (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein absorbance was read at 562 nm using a Spectramax i3x Microplate reader (VWR). 10 µg of total proteins were denatured at 95°C for 5 min in 100 mM dithiothreitol (DTT ...