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Citations for VWR :
1 - 50 of 197 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... MEF cells were passaged in alpha-minimal essential medium (alpha-MEM; Hyclone Laboratories, Logan, UT, USA) supplemented with 10% FBS (VWR, Radnor, PA, USA) and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was later thawed and homogenized in RIPA buffer (VWR; Atlanta GA) via agitation on ice for 30 minutes before centrifugation at 23,000 x g for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Plates were sealed with a gas-permeable microplate adhesive film (VWR, USA), and then optical density and fluorescence were monitored for 48 hours in an Biotek Synergy 4 plate reader (BioTek ...
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bioRxiv - Microbiology 2020Quote: ... Plates were sealed with a gas-permeable microplate adhesive film (VWR, USA), and then optical density and fluorescence were monitored for 20 hours in a Tecan Infinite F200Pro (Tecan Life Sciences ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were sealed with a gas-permeable microplate adhesive film (VWR, USA), and then optical density at 600 nm (OD600 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plate was covered with a sterile gas permeable rayon film (VWR) and incubated overnight at 37°C with shaking ...
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bioRxiv - Microbiology 2021Quote: ... Plates were sealed with a gas-permeable microplate adhesive film (VWR, USA), and then optical density and fluorescence were monitored for 24-72 hours in an Biotek Synergy H1M plate reader (BioTek ...
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bioRxiv - Biochemistry 2021Quote: ... sterility was maintained with standard techniques and a gas permeable rayon film (VWR). The liquid culture plate was incubated for 5 hours at 37 °C and 250 rpm shaking ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% heat inactivated horse serum (VWR, cat#103219-558, Suwanee, GA,) and 2 mM L-glut (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... Pan-flavivirus E protein antibody (1:100, VWR Enzo, 76285-044), GFAP (1:250 ...
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bioRxiv - Microbiology 2022Quote: ... Dormant spores were purified by suspending the pellets in 20% Nycodenz (VWR, Atlanta, GA) followed by 50% Nycodenz density gradient centrifugation at 14000 g for 45 minutes ...
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bioRxiv - Genomics 2024Quote: ... antibody overnight followed by incubation with protein A coupled Sepharose beads (VWR). Samples were washed with lysis buffer ...
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bioRxiv - Bioengineering 2024Quote: ... 10% fetal bovine serum (Atlanta Biologics, Flowery Branch, GA) and 1% penicillin-streptomycin (VWR, Radnor, PA). The bone marrow from the femur and tibia was flushed out with 10 mL wash media and pipetted to generate a homogeneous suspension ...
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bioRxiv - Microbiology 2024Quote: ... the extracted biofilm matrix was lyophilized using an ALPHA 1-4 LDplus freeze dryer (Christ, VWR) and kept at −80 °C until sample preparation for proteomics.
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bioRxiv - Biophysics 2024Quote: ... retention time = 6 min) The peptide was lyophilized (Christ Freeze Dryer Alpha 2-4 LDplus, VWR) and stored at -20 °C ...
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bioRxiv - Immunology 2020Quote: ... After 24 hours tissues were embedded in Optimal Cutting Temperature (OCT) compound (VWR Chemicals, Atlanta, GA, USA) and snap frozen on a metal bar cooled by dry ice ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The library was cultured in a 96-well deep well plate sealed with a gas permeable membrane (VWR, USA). The plate was shaken (700 rpm ...
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bioRxiv - Synthetic Biology 2024Quote: ... in PBS buffer (pH 7.4) was coated onto a high-binding 96-well plate (VWR) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes prior to incubating for 72 hours at 37°C in a 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). Following incubation ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated for 72 hours at 37°C in 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). All wells had a DMSO concentration of 0.5% v/v with drugs MRX-2843 (25 – 800 nM) ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were rinsed thoroughly in RO water and dried with nitrogen gas before storing in the dark at room temperature in a Tupperware container with Drierite desiccant (VWR).
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bioRxiv - Bioengineering 2024Quote: ... the two compounds selected as extraction baseline were the commonly used straight-chain alkane n-dodecane (≥99%, VWR International, France), which forms an overlay above algal cultures and is known to continuously “milk” terpenoid metabolites from living cultures [28] ...
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bioRxiv - Immunology 2024Quote: ... LUMITRAC 600 high-binding white polystyrene 384-well microplates (Greiner Bio-One, #781074; VWR, #82051-268) were first pre-coated overnight with 10uL per well of antigen (Ag) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubating in 5% normal horse serum for 60 min to block nonspecific binding (VWR, 102643-676). Afterwards ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Biophysics 2024Quote: ... Storage solution was removed and the resin was washed with 3×1.0 mL of target-binding buffer (25 mM HEPES pH 7.4, 150 mM NaCl, 0.05% tween-20 (VWR)) ...
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bioRxiv - Neuroscience 2024Quote: ... Premixed gases were delivered into the chamber through the gas inlet at a flow rate of ∼700 mL/min (controlled by a flow meter, VWR model #FR2A138VVT-VW) from gas tanks (Airgas ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...