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Citations for VWR :
651 - 700 of 717 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Transwells with fixed and stained infected epithelia were removed from their supports with a scalpel and mounted on glass slides with VECTASHIELD® Antifade Mounting Medium (VWR). Images of infected cultures were acquired with a Leica Stellaris 8 Confocal Microscope (40× magnification ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Genomics 2022Quote: ... The slide was removed from the holder and air dried before mounted with 85% glycerol and covered with a cover slip (VWR, USA) prior to imaging ...
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bioRxiv - Synthetic Biology 2022Quote: ... for 30 mins in 150 μl lysis buffer containing 8 mg/mL lysozyme (from chicken egg white, #76346-678, VWR, USA), 10 mM Tris HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... 8 µm serial sections of the embedded ovary from an individual were mounted on polysine-coated Microslides (VWR International, Radnor, US). For UH two individual slides containing whole cross sections of UH per animal were assessed ...
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bioRxiv - Neuroscience 2024Quote: ... We obtained HEK-293 cells from Jong Yun Lab (Penn State College of Medicine) and cultured them in DMEM (VWR International) supplemented with 10% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 – 20 mosquitoes were sorted into a behavioral container made from a 32-ounce HDPE plastic cup (VWR #89009-668). The behavioral container was prepared by cutting a 10 cm hole in the lid with a razor blade and covering the cup with a 20 cm x 20 cm piece of white 0.8 mm polyester mosquito netting (American Home & Habit Inc ...
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bioRxiv - Genomics 2022Quote: Colonic tubes were dissected within minutes of sacrificing mice and tissues dried from excess fluids and embedded in Optimal Cutting Temperature (OCT, Fisher Healthcare, USA) in large molds (VWR, USA) pre-filled with OCT ...
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bioRxiv - Microbiology 2023Quote: ... Microbial samples grown on the agar medium were cut directly from the Petri dish and transferred to a microscope slide (VWR, USA), previously covered with a double-sided conductive copper tape (3M) ...
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bioRxiv - Microbiology 2024Quote: ... spun for 30min at 4°C 4,000g and protein purified from supernatant using glutathione agarose beads (Pierce, 16100) and elution with reduced L-Glutathione (VWR, 76177-906). Protein purity was confirmed on a protein gel and concentration was determined using a Nanodrop ...
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bioRxiv - Immunology 2024Quote: Lungs were harvested from control and pDC-less mice 8 days after IAV infection and kept with 10% buffered formalin (VWR Chemical) for 24 hours ...
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bioRxiv - Physiology 2024Quote: ... using a FastPrep 24 (M.P. Biomedicals, Santa Ana, CA, USA) with bead-beating tubes (16466– 042) from (VWR, Radnor, PA, USA) and zirconium ceramic oxide bulk beads (15340159 ...
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bioRxiv - Physiology 2024Quote: ... they were individually transferred to single wells of a 96-well single-cell-sequencing plate from VWR (Cat. No. 82006-636) with 2 ul of RNAse inhibitor mix (containing 0.12 ul Triton X-100 10 % ...
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bioRxiv - Bioengineering 2024Quote: ... MA). Anti-rabbit secondary antibody Alexa Fluor® 488 anti-IgG donkey (cat. #102649-730) was purchased from VWR (Radnor, PA).
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bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
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bioRxiv - Microbiology 2021Quote: ... Agar overlay lawns for passages were prepared by aliquoting 100 ul of host overnight culture (4ml 2216MB, colony inoculum from streak on 2216MB with 1.5% Bacto Agar, shaken overnight at RT at 250rpm on VWR DS500E orbital shaker) onto a standard size bottom agar plate and adding 2.5 ml of molten 52°C top agar as in the bait assay ...
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bioRxiv - Systems Biology 2020Quote: ... cells were removed from their liquid media by flowing the liquid cultures that contained cells through a 0.45−μm pore filter (VWR, cellulose-acetate membrane). To ensure and verify that there were no cells remaining in the filtered media ...
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bioRxiv - Immunology 2021Quote: VeroE6 cells were obtained from ATCC (clone E6, ATCC, #CRL-1586) and cultured in complete DMEM medium consisting of 1x DMEM (VWR, #45000-304), 10% FBS ...
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bioRxiv - Plant Biology 2021Quote: ... Subsamples of each roughly ground material were sampled from well-mixed media and ground down to micron size using a VWR high throughput homogenizer (VWR, Radnor, PA).
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bioRxiv - Bioengineering 2020Quote: ... and particles were removed from PDMS chip and glass substrate (76 x 26 x 1 mm microscope slides, VWR International GmbH, Germany) via isopropanol washing and drying with pressurized air ...
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bioRxiv - Biochemistry 2020Quote: ... Two sets of spectra ranging from 230 to 750 nm were recorded simultaneously using two UV-3100PC UV/Vis spectrophotometers (VWR, Radnor, PA). In the first device ...
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bioRxiv - Plant Biology 2021Quote: ... Bulk seed was collected from the third generation and screened to homogenize seed size with 350 μm and 250 μm sieves (VWR, Pennsylvania, USA), retaining the middle fraction.
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bioRxiv - Immunology 2021Quote: VeroE6 cells were obtained from ATCC (clone E6, ATCC, #CRL-1586) and cultured in complete DMEM medium consisting of 1x DMEM (VWR, #45000-304), 10% FBS ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Immunology 2021Quote: VeroE6 cells were obtained from ATCC (clone E6, ATCC, #CRL-1586) and cultured in complete DMEM medium consisting of 1x DMEM (VWR, #45000-304), 10% FBS ...
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bioRxiv - Immunology 2021Quote: VeroE6 cells were obtained from ATCC (clone E6, ATCC, #CRL-1586) and cultured in complete DMEM medium consisting of 1x DMEM (VWR, #45000-304), 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... a 1 μl sample was taken from the tube and pipetted onto a microscope slide (VWR micro cover glass 24x60mm No.1) and a coverslip added (VWR micro cover glass 24x30mm No.1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cancer Biology 2023Quote: Five-micron tissue sections were cut from FFPE TMA tissue blocks and adhered onto VWR Superfrost Plus Micro Slides (VWR, 48311-703). After sectioning ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each plant was sprayed for one minute and the collected flow through from each plant were first strained using a 40 μm cell strainer (VWR 76327-098) to remove large microorganisms and large particles ...
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bioRxiv - Developmental Biology 2023Quote: ... and rehydrated in a series of graded ethanol washes (100%, 95%, 85%, 70%, and 50%) that were diluted from 100% ethanol (VWR, 71002-512). Antigen retrieval was performed using an Antigen Unmasking Solution according to the manufacturer’s instructions (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were then mechanically sleep-deprived 3 sec every min from ZT12-ZT0 using a vortexer (VWR DVX-2500 Multi-tube vortexer). Flies were then taken out of the individual tubes between ZT23 and ZT0 and placed into standard imaging chambers using a mouth pipette ...
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bioRxiv - Molecular Biology 2024Quote: ... SC-islets were then shipped from Vancouver to Ottawa and maintained in Stage 6 media [CMRL-1066 (VWR, #CA45001-114; Radnor, PA) containing 20 g/L bovine serum albumin (Sigma-Aldrich #10775835001) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell harvesting involved a PBS wash followed by 1 mL of a homemade lysis buffer containing a base buffer (50 mM Tris-HCl in DEPC-treated water, pH 7.4; 250 mM KCl; 25 mM MgCl2; all ingredients from VWR, Radnor, PA, USA) and the following chemicals ...
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bioRxiv - Immunology 2024Quote: Single cells prepared from tumor tissues were counted and 2-5 million cells were plated on a 96-well plate (VWR, round botom) and washed with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Genomics 2024Quote: ... samples were subjected to 30 minutes of heating by removing tubes from the incubator and placing them in heat blocks (VWR 13259-254) that were pre-heated to one of three temperatures (30 ...
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bioRxiv - Microbiology 2024Quote: ... Six clams taken from the acclimation aquarium were crushed together for 2 min at maximum speed using the Stomacher® 3500 instrument (VWR, Italy). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... Extracellular parasites were removed by washing with HBSS and intracellular parasites were released from host cell by scraping with a cell scraper (VWR, 734-2602), passages through 26G needles and finally filtered on a fiber glass wool column ...
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bioRxiv - Microbiology 2024Quote: ... We introduced 8 male and 8 female flies into an arena constructed from a modified 6-oz square-bottom fly bottle (VWR 75813-140) (the ‘top’ ...
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bioRxiv - Neuroscience 2024Quote: ... an unsorted sample was taken from the input chamber of the cartridge using a 200 µl gel loading tip (VWR, 732-2671). The sort was performed in the MACSQuant Tyto cell sorter using software 1.0.0.
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bioRxiv - Pharmacology and Toxicology 2021Quote: The active fractions from the NC-MT assay were separated in a 10% SDS gel and stained with coomassie blue (InstantBlue, VWR Chemicals, Darmstadt, Germany) or silver stain kit for mass spectrometry (Pierce/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow was collected via centrifugation from the femur and tibia of 8-week-old male C57BL6/J mice and cultured in αMEM (VWR, Radnor, PA, USA) supplemented with 10% fetal bovine serum VWR ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Membranes that were electrospun from 15 wt% of PVDF without dyes onto a layer of PP fabrics (VWR® Basic Protection Face Mask) were also fabricated for comparison (i.e. ...
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bioRxiv - Microbiology 2020Quote: ... Synthesis of the first-strand cDNA from the extracted total RNA was conducted using a QuantaBio qScript cDNA synthesis kit (VWR Canada, Edmonton, AB). Primers and gBlocks were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2021Quote: GC-376 (CAS: 1416992-39-6) was purchased from Enovation chemicals (Green book, NY) and dissolved in deionized water (HyClone, VWR, West Chester, PA) to the desired concentration.