Labshake search
Citations for VWR :
601 - 650 of 717 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... sorted RBCs were removed from the microfluidic device and transferred to a 96-well flat-bottom plate (VWR International, LLC). Samples of sorted cells from each outlet were divided evenly and placed into two separate wells to provide cell images captured with variations in well location distribution and automatically determined imaging parameters (e.g ...
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bioRxiv - Microbiology 2020Quote: 250 μL of virus transport medium (VTM) from flocked NP swabs were adjusted to 1.0 mL with 0.1% Triton X-100 (proteomics grade, VWR: 97063-864) diluted in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Tissue specimens were transported directly from surgical theatre to the laboratory in ice-cold sterile Phosphate Buffered Saline (VWR, UK). LVs were identified and isolated along with a small amount of surrounding tissue under a dissection microscope within a sterile laminar flow cabinet (Fig 1 A-C) ...
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bioRxiv - Bioengineering 2022Quote: ... channels were gently removed from the adhesive carrier sheet using fine-tipped tweezers and sonicated in ethanol (VWR, PA, USA) for 5 min ...
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.4 µm TC Plate Insert with a PC Membrane 6 well plate (thinserts) was purchased from VWR (Radnor Pennsylvania, USA). PD-L1 ...
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bioRxiv - Biochemistry 2022Quote: ... The handling of this liquid and the titration of the metals from a 96-well deep block (VWR, #82051-260), was performed by a EvoWare v2.7 scripted Tecan Freedom Evo 100 base equipped with a Tecan fixed-tip liquid handling arm ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We removed nearly all dissection/fixation solution from the sample and picked up the coverslip with a HistoBond microscope slide (VWR). Samples were then frozen on dry ice ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... cecal contents from specific-pathogen free (SPF) mice were resuspended in 10% weight/volume (w/v) Bacto Brain Heart Infusion (BHI) (VWR) supplemented with 0.05% w/v L-cysteine (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The supernatant from the second and third digestions were then pooled and strained through a 100 μm cell strainer (VWR). Pooled and digested villous suspensions were then centrifuged at 4°C at a speed of 1200 rpm for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Effluent was collected from a steam-sterilized sample valve in the bottom of the bioreactor into sterilized plastic Nalgene bottles (VWR) equipped with a gas exhaust filter ...
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bioRxiv - Neuroscience 2022Quote: ... 40 ¼m coronal sections were cryostat cut through the rostrocaudal extent from cortex to the brainstem and slide-mounted (Superfrost Plus, VWR). Mounted sections were then stored at −80 °C for subsequent immunohistochemistry (IHC ...
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bioRxiv - Plant Biology 2022Quote: ... were punched from the basal region using a sterile biopsy punch (pfm medical) and transferred to a 96-well plate (VWR). Bioluminescence was measured in a FLUOstar Omega plate reader (BMG Labtech).
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bioRxiv - Molecular Biology 2022Quote: ... 0.23 µl fluorescein (100X, prepared according to “Fluorescein preparation for naica® system” from Stilla Technologies) (0681-100G, VWR Chemicals), 1 µl of diluted RNA sample (or a single cell) ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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bioRxiv - Pathology 2024Quote: Six μm sections of formalin-fixed paraffin-embedded (FFPE) tissue from the dorsolateral prefrontal cortex (Brodmann Area 9) were de-paraffinized with CitriSolv (Xylene substitute, VWR) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Immunology 2023Quote: ... Analytical grade ammonia solution (28%, GPR RECTAPUR®) and acetic acid glacial (AnalaR® NORMAPUR®) were obtained from VWR Chemicals.
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Label-free digital holotomography reveals ibuprofen-induced morphological changes to red blood cellsbioRxiv - Cell Biology 2022Quote: Whole blood was freshly obtained with the consent of a healthy donor from a finger prick with safety lancets (VWR). Sickle cell trait (SCT ...
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bioRxiv - Biophysics 2022Quote: Muscle tissue culture molds were fabricated from polymethyl methacrylate (PMMA) and glued onto a microscopy cover glass (VWR, Radnor, USA) as previously described[22] ...
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bioRxiv - Neuroscience 2023Quote: Hypothalamic progenitors differentiated from the HUES9-POMC-GFP cell line were re-plated onto 24 mm round glass coverslip (VWR) that has been pre-treated with 5 M hydrochloric acid and double coated with both 0.01% poly-L-ornithine hydrobromide (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Neuroscience 2023Quote: ... von Frey filaments were obtained from Ugo Basile and were calibrated in the lab using a weigh scale (VWR, 314AC) to 0.01 grams ...
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bioRxiv - Biochemistry 2024Quote: ... was supplied from an LA755 Elga purification system (Elga Lab water, High Wycombe, USA) and isopropyl alcohol (IPA) and ethanol were obtained from VWR International (Radnor ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Genomics 2024Quote: ... Enzymes were purchased from New England Biolabs (NEBNext Companion Module #E7180) and Omega Bio-Tek Mag-bind TotalPure NGS magnetic beads were from VWR International ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Oxford Nanopore Rapid Barcoding Kit and R10.4 flow cell were purchased from VWR (USA, #SQK-RBK114.96 and #FLO-MIN114). For the short-reads ...
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bioRxiv - Microbiology 2024Quote: ... pOXA-48-carrying and pOXA-48ΔlysR-carrying KPN15 and CF13 were performed in the presence of the Iron Chelator 2,2-Bipyridyl from VWR (ref. 30569.09). Concentrations of 2,2-Bipyridyl ranging from 0.016 mM to 0.5 mM were tested ...
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bioRxiv - Biochemistry 2024Quote: ... Axygen 12-Channel Through (cat#732-1390) and reagent reservoirs (cat#89094-666 and cat#89094-668) were obtained from VWR International GmbH (Bruchsal ...
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bioRxiv - Systems Biology 2024Quote: ... then took a single colony from each strain and precultured in 200µL TSB (Tryptic soy broth non-animal origin irradiated, VWR #84674.0500) in a 96-well 500µL deep plate (Masterblock 96well ...
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bioRxiv - Synthetic Biology 2024Quote: ... with a confluence of 80-90% were washed twice with PBS and detached from their respective flasks using Trypsin 0,25% - EDTA in HBSS (VWR, Germany). The cell suspension was washed with medium ...
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bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
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bioRxiv - Cell Biology 2024Quote: ... dissolved in a 50:50 (v/v) mixture of chloroform and ethanol (pharma grade reagents from VWR Chemicals, Darmstadt, Germany) was prepared by stirring at room temperature overnight ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from total cells and eluates from immuno-precipitations were separated by standard SDS-PAGE and transferred to PVDF membranes (#10600023, VWR, USA) for the detection of Mup1-GFP ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using 500 μl of Trizol reagent and precipitated from the aqueous phase with 500 μl of Isopropanol (VWR Medicals) and 1 μl of glycogen (20 μg/μl ...
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bioRxiv - Bioengineering 2020Quote: ... biofilm from the wet-end (BF) and the effluent from the biological treatment plant (BTP) were individually microbiologically enriched in M9 media (VWR, USA) (Miller ...
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bioRxiv - Bioengineering 2021Quote: ... Sorted RBCs suspended in 10 µL of HBSS with 0.2% Pluronic-F127 from each outlet are removed by pipetting and placed in a 96 well plate (VWR International, LLC) for imaging.
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bioRxiv - Bioengineering 2021Quote: ... Dye-loaded nanocarriers were purified from free dye by dialysis or 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Microbiology 2021Quote: ... Optical density was followed over the course of the experiment by measuring it directly from the Hungate tube with a Biowave cell density meter CO8000 (VWR International). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... Organic solvents for extraction and liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis were purchased from VWR (ethyl acetate (EtOAc), HiPerSolv Chromanorm purity ...
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bioRxiv - Bioengineering 2021Quote: ... BM-MSCs isolated from heparinized bone marrow aspirates by seeding 50,000 white blood cells/cm2 on two-chamber CellStacks (Corning/VWR, Ulm, Germany) in α-MEM (Lonza ...