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Citations for VWR :
501 - 550 of 622 citations for 2S 2 Boc amino undecanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Parasites were allowed to invade the HFFs for 2 hours before the media on one set of wells was supplemented with either 0.003µM monensin (VWR, BUF074) or vehicle control (70% ethanol) ...
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bioRxiv - Genomics 2024Quote: ... Washed beads were resuspended in 100 µL of Elution Buffer (2% (v/v) N-lauryl sarcosine (VWR; 100218-364), 10 mM EDTA ...
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bioRxiv - Bioengineering 2024Quote: ... approximately 5×106 cells were removed from the bioreactor and centrifuged at 200 x g for 2 min (VWR). The supernatant was discarded and the cell pellet was frozen in liquid nitrogen and stored at -80°C for further analysis.
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bioRxiv - Biophysics 2024Quote: Glass-bottom 384-well plates (Brooks, MatriPlate MGB101-1-2-LG-L) and cover glass (VWR, USA, 48393-251) were passivated with PEG-silane (MW = 5 kD ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Bioengineering 2021Quote: ... An array of 2 mm holes was punched in the channel outlets using a biopsy punch (VWR, Cat# 21909-132). A hole was drilled on a plate lid (VWR ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with PBST and then incubated with 4′,6-diamidino-2-phenylindole (DAPI, 5 µg/mL in PBS) (VWR) and phalloidin (1.5 µM in DMSO ...
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bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
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bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
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bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover for 48 hours before passage into new 10 cm plates containing DMEM media supplemented with 2 mg/ml Geneticin sulfate (G418; VWR). Following 7 days of selection ...
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bioRxiv - Microbiology 2021Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Microbiology 2020Quote: A Ninjaflex 2 mm thick gasket was 3D printed onto a 50 mm × 75 mm × 1 mm glass slide (VWR) using a Makerbot 2× printer (Appendix I) ...
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bioRxiv - Bioengineering 2021Quote: ... well plates were washed two times with MilliQ H2O and then stained with 400 μL of 2% w/V alizarin red S (VWR) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Biophysics 2021Quote: ... The sonicator probe (SFX250 Sonifier with 1/2” horn and 3 mm tapered microtip, Branson Ultrasonics, VWR International, Radnor PA) was then positioned in the chamber with its tip 2 mm above the coverslip ...
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bioRxiv - Microbiology 2020Quote: ... Transfections were conducted by electroporation using a BioRad Gene Pulser Xcell at 1.5 kV and 25 µF with 2 mm electroporation cuvettes (VWR international) in Cytomix buffer (10 mM KH2PO4/K2HPO4 (pH 7.6) ...
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bioRxiv - Genetics 2020Quote: ... HEPES pH 7.8 and 0.00004% methylene blue in autoclaved reverse osmosis water) and mounted them on coverslip bridges (2 x 22 mm square glass coverslips (16004-094, VWR) glued together on either side of a 24 x 60 mm glass cover slip (12460S ...
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bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
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bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
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bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: The brain and lung tissue were transferred to a cold room and subjected to homogenization using a 2 ml Dounce homogeniser (VWR, Tissue grinders ...
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bioRxiv - Biochemistry 2023Quote: ... were obtained from MilliporeSigma. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride (Cat. # K831, CAS: 51805-45-9) was purchased from VWR. Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine culture of E.coli for cloning purposes was performed in Miller’s LB Broth (Fisher BP1426-2) in 14mL Falcon tubes (VWR 60819-761) at 37°C on a rotating drum ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were transferred to a 2 mL tube pre-filled with 2.8 mm ceramic beads (VWR, Cat. No. 10158-612), flash frozen on dry ice ...
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bioRxiv - Cell Biology 2023Quote: ... CO2-anethetized male or female flies that had eclosed 2 days earlier were mounted on double-sided sticky tape and wings coated in Voltalef 10S halocarbon oil (VWR). Movements of mitochondria (labeled with mito-GFP under the control of the neuronal driver appl-GAL4 ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µL of each was added to 2 mL cultures of attached cells in 6-well plates (#10861-554, VWR) containing 0.2 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
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bioRxiv - Synthetic Biology 2023Quote: Sender strains and receiver strains were inoculated in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in a 2 mL deep-well 96-well plate (VWR), covered with a “Breathe Easier” membrane (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Bioengineering 2024Quote: ... to measure the radial expansion for each graft whereas thickness was measured on 1-2 mm length graft cross-sections imaged under phase contrast microscopy (VWR). Burst pressure was defined as the highest pressure recorded on the pressure monitor during the burst experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... polyallomer tubes were placed in an ice bucket and the top 1 mL (termed top fraction) was carefully removed with a pipettor and placed in a 2 mL microtube containing 1 mL Trizol (VWR). The remaining sucrose was carefully poured/discarded ...