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Citations for VWR :
451 - 500 of 622 citations for 2S 2 Boc amino undecanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... disodium;4-[3-pyridin-2-yl-6-(4- sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate hydrate (Ferrozine, VWR) All media components were autoclaved or sterilized using 0.2 μm PES filters.
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 15,000 rpm for 2 min and filtered through a 0.2 μm cellulose acetate membrane (VWR). Then ...
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bioRxiv - Microbiology 2024Quote: ... of the sample weight for 2 minutes at maximum speed using the Stomacher® 3500 (VWR, Italy). To assess the microbial load ...
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was then normalized to 2 ng/µL by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot.
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bioRxiv - Molecular Biology 2024Quote: ... blood was washed out with 2 ml PBS and 1 ml of latex dye (VWR, LL0057-1L) was slowly and steadily injected into the left ventricle with an insulin syringe ...
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was then normalized to 2 ng/µL by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot.
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bioRxiv - Cell Biology 2020Quote: ... pHrodo™ iFL Red STP Ester was diluted to 2 mM in DMSO (VWR Life Science, 97063-136). Antibody was brought up in DPBS (2 mg/mL final concentration for Dll4 polyclonal antibody [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Microbiology 2021Quote: ... and 300μL of XTT solution was added to each well (0.5mg/mL XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] (VWR) with 25μM menadione in PBS) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Synthetic Biology 2022Quote: ... Isolated colonies were picked and grown in 2 ml of growth medium in culture tubes (VWR, 60818-667) for 12-24 hours at 30°C and 220 rpm in an orbital shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized in a 2 ml lysis buffer using a Dounce tissue grinder (7 ml, VWR). After addition of a 1.8 M sucrose solution (4 mL) ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Genomics 2022Quote: ... MB135-iDUX4/ZSCAN4-mCherry and MB135-iDUX4 myoblasts were additionally maintained in 2 μg/mL puromycin dihydrochloride (VWR). MB135-iFLAG-SRSF3-FL and -TR myoblasts were additionally maintained in 10 μg/mL blasticidin S HCl (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... several milligrams of raw curcumin were dispersed in water and filtered using a 2 μm filter paper (VWR® Grade 410 ...
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bioRxiv - Microbiology 2024Quote: ... duplicate 470 µL aliquots of each culture were removed from the chamber in 2 mL eppendorf tubes (VWR), which were sterilized with SporGon ...
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bioRxiv - Neuroscience 2023Quote: ... animals underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a ‘Karlsson-Schultz’ perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... The remainder of the suspension was transferred to a 2 ml glass Dounce homogeniser (VWR. Cat# 89026-386) and subjected 25 up and down cycles of homogenization ...
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bioRxiv - Molecular Biology 2023Quote: ... Expression of pXLone-dCas9-DNMT3A3L-P2A-EGFP was induced with 2 μg/mL Doxycycline (VWR, Cat# AAJ67043-AD) and GFP-fluorescent cells were enriched by fluorescence-activated cell sorting ...
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bioRxiv - Microbiology 2024Quote: ... RLMVECs assayed for Mx1/2/3 expression during SEOV infection were fixed using 4% paraformaldehyde (VWR, 97064-606) solution in sterile PBS for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: A bubbling system was set up with 2 flasks of 1 M NaOH (VWR, catalog no. 97064-476) DI H2O (PURELAB Flex ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The stable cell lines were plated at 2×106cells/ 35mm cell culture dish (VWR, Radnor, PA, cat # 82050-538). After 24hrs ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were diluted to 2 M urea / 0.1 M NH4HCO3 with 140 μl of HPLC grade water (VWR). Digest with mass-spec grade trypsin (Serva ...
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bioRxiv - Neuroscience 2022Quote: After MRI scanning the shanks were carefully removed and brains were transferred to 2% dimethyl sulfoxide (DMSO, VWR, USA) for cryoprotection for 1-2 days prior to cryosectioning ...
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bioRxiv - Cancer Biology 2020Quote: ... which is prepared by dissolving 2% wt/vol sodium alginate (Protanal LF200S) in water and by adding 0.5mM SDS surfactant (VWR International ...
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Cell Biology 2020Quote: ... Fat droplets were stained through a freshly filtered solution of 0.3 % (w/v) Oil Red O dissolved in 60% (v/v) 2-propanol (VWR). Afterwards ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphoproteome samples were resolubilized in 50 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was also transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Plant Biology 2022Quote: ... The reference proteome sample was resolubilized in 30 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Neuroscience 2024Quote: ... was added to 2ml tubes containing Precellys Steel beads (2.8 mm ‘reinforced’ 2 ml tubes, pre-filled with steel beads purchased from VWR International Ltd ...
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bioRxiv - Bioengineering 2024Quote: ... The pH was monitored starting from 6.49 and reducing to 3.02 using a digital pH meter (accuracy ± 0.1% ± 2 digits, VWR pH110, Belgium).
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bioRxiv - Neuroscience 2023Quote: ... the canary underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Inoculums of 100 µL were delivered by intra-gastric gavage using a 2-inch-long plastic feeding needle (VWR) connected to a 1 mL syringe (Becton Dickinson) ...
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bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).
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bioRxiv - Microbiology 2023Quote: ... and adding sterile filter double distilled water (liquid medium) or autoclaved molten 2% DNA pure agarose (VWR, Radnor, PA) (agarose plates ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Neuroscience 2023Quote: Primary microglial cells were seeded at a density of 2 x 105 cells on 12 mm glass coverslips (VWR) coated with poly-L-lysine (0.1 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... myofibril pellets were resuspended in 300 μL of ice-cold wash buffer (Buffer 2: 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 1 mM DTT; all chemicals from VWR), and tubes were centrifuged at 3,000 g for 10 minutes at 4ºC ...