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Citations for Zymo Research :
101 - 150 of 417 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of each module (PCR product + Zymo Shield) (6 in total ...
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bioRxiv - Microbiology 2020Quote: ... Amplified insertions were PCR purified (DNA purification kit, Zymo) and subjected to Sanger sequencing by MCLAB ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were purified (Zymo Research Clean and Concentrator kit) and pooled for sequencing on an Illumina MiSeq with 150 bp single end reads ...
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bioRxiv - Plant Biology 2021Quote: ... and Zymo OneStep PCR Inhibitor Removal Kit (D6030, Zymo) to obtain high quality genomic DNA ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were column cleaned (Gel cleanup kit, Zymo), and pooled based on relative concentration ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of 2X ZymoTaq PCR Mix (Zymo Research), PCR primers ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was purified using a kit (Zymo Research DNA Clean & Concentrator™ Cat ...
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bioRxiv - Plant Biology 2021Quote: ... The ITS regions were amplified by qPCR using ITS-specific primers provided by the Femto(tm) Fungal DNA Quantification Kit (Zymo Research) to quantify the relative abundance of fungal DNA following the manufacturer’s recommendations for the three biological replicates ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Microbiology 2022Quote: ... or by RT-qPCR (IDT technologies, Charité kit) after RNA extraction with TRI reagent and Direct-Zol RNA miniprep kit (Zymo Research).
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bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research, Irvine, CA, USA), as previously described (Kongprajug et al. ...
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bioRxiv - Plant Biology 2020Quote: ... a qRT-PCR product was purified from a complete PCR reaction using the Zymo DNA Clean & Concentrator™-5 kit (Zymo Research, Irvine, CA) and eluted in 10 μl of nuclease-free water ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were purified using DNA Clean & Concentrator Kit (Zymo) and sent for Sanger sequencing to obtain trace files (Genewiz) ...
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bioRxiv - Plant Biology 2020Quote: ... and the resulting PCR products were purified (Zymo, Irvine,CA), and sequenced (GENEWIZ ...
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified using DNA spin column (Zymo Research). Single strand preamplifier was synthesized using Guide-it Long ssDNA Production System (Takara Bio ...
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bioRxiv - Genomics 2019Quote: ... PCR products were cleaned by DNA Clean & Concentrator kit (Zymo) and Sanger sequenced to verify the barcode sequences.
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bioRxiv - Evolutionary Biology 2020Quote: ... These PCR products were purified on silica columns (Zymo Research) and sequenced for 100 base single reads on an Illumina NextSeq instrument ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using the DNA concentrator kit (Zymo) following the manufacturer’s recommendations and eluted in 30 μL ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were cleaned by spin column purification (Zymo, Inc.) and DNA eluted in 20 uL of Tris-HCl ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were cleaned using DNA Clean & Concentrator (Zymo). The ligation product was assembled as following ...
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bioRxiv - Developmental Biology 2023Quote: ... Then PCR products were column purified (Zymo Research, Cat# D4004) and Sanger sequenced (Table S1 ...
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bioRxiv - Molecular Biology 2023Quote: ... purification of PCR products (DNA Clean & Concentrator-5, Zymo Research), gel extraction (QIAquick Gel Extraction Kit) ...
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bioRxiv - Microbiology 2021Quote: ... were thoroughly mixed 1:1 with 2X DNA/RNA Shield™ and incubated at RT for 20 min for inactivation and vRNA was extracted using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... was used for bacterial 16S ribosomal RNA targeted sequencing and custom 16S primers were utilized to amplify the V3-V4 region (Zymo Research, Irvine, CA). Real-time PCR was then used to prepare the sequencing library and final qPCR fluorescence readings were pooled together according to equal molarity and the final pooled library was cleaned using the Select-a-Size DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were purified two times over silica columns (Zymo Research) and quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... the OneStep PCR inhibitor removal kit (Zymo Research, Irvine, CA, USA) was employed ...
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bioRxiv - Genetics 2020Quote: ... PCR amplicons were gel-purified (Zymoclean Gel DNA recovery kit, Zymo), quantified (Nanodrop 1000 spectrophotometer ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products were concentrated using DNA Clean and Concentrator (Zymo, D4013) followed by 0.8X SPRIselect (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was cleaned (Zymo DNA clean and concentrator-5) and then sent to Genewiz for 2 x 250 bp amplicon sequencing (Amplicon-EZ service) ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... PCR products were purified two times over silica columns (Zymo Research) and quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Genomics 2020Quote: ... Amplicons were PCR-purified using (DNA clean and concentrator kit, Zymo) and cloned into 100ng of BbsI linearized pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W vector by Gibson Assembly Cloning (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... PCR products were purified by DNA clean & concentrator-25 (Zymo Research). In vitro transcription was performed by incubating 5 μg of transcription template with NTP ...
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bioRxiv - Microbiology 2021Quote: ... DNA was purified using One Step PCR Clean Up (Zymo Research). The DNA was quantified using Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR product was purified (Zymo DNA Clean and Concentrator Kit, D4013) then used for in vitro RNA synthesis using a MEGAshortscript T7 Transcription Kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified two times over silica columns (Zymo research) and DNA concentration was quantified using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Genomics 2022Quote: ... the DNA was directly extracted using PCR Purification Kits (Zymo, D4013).
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bioRxiv - Cancer Biology 2022Quote: ... PCR inhibitors were removed from the resulting gDNA (Zymo Research, D6030) and the concentration of the resulting gDNA was measured using the Qubit dsDNA HS assay kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The final amplicons were subjected to PCR clean up (Zymo D4004) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified two times over silica columns (Zymo Research) and the DNA concentration was measured by qPCR using the KAPA SYBR FAST qPCR Master Mix (Kapa Biosystems ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Microbiology 2020Quote: ... 75μl supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2019Quote: RNA was isolated from normal bladder tissues and from VM-CUB1 and RT-112 cells using Direct-zol RNA MiniPrep kits (Zymo Research, Irvine, CA). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicons of the target genes were checked with gel electrophoresis to assure the absence of primer dimmers and then purified using DNA Clean & Concentrate™ column (Zymo Research, Orange, CA). The purified DNA was serially diluted to 102 ...