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Citations for Zymo Research :
1 - 50 of 417 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
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bioRxiv - Cell Biology 2024Quote: ... Labeled 3p-hpRNA (AF647-3p-hpRNA) was purified using Zymo Direct-zol Miniprep Kit (Zymo Research, R2051) and eluted in physiologic water (InvivoGen ...
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bioRxiv - Microbiology 2023Quote: ... and the Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Pathology 2023Quote: ... The DNA samples were then prepared for targeted sequencing with Quick-16S Primer Set V3-V4 Library (Zymo Research, Irvine, CA). The sequencing library was prepared using innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and limit PCR chimera formation ...
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bioRxiv - Neuroscience 2022Quote: ... The DNA samples were prepared for targeted sequencing with the Quick-16S NGS library Prep kit and 16S primer set V3-V4 (Zymo Research Irvine, CA). The final library was sequenced on Ilumina MiSeq with a V3 reagent Kit (600 cycles) ...
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bioRxiv - Bioengineering 2024Quote: ... Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research, Irvine, CA). The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity ...
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bioRxiv - Neuroscience 2019Quote: Methylated and unmethylated DNA sets (Cat# D5017, pUC19 DNA set, Zymo research) were added as spike-in controls (2 ng spike-in control in 1 µg DNA ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA for quantitative RT-PCR assays was isolated using the Quick-RNA Microprep kit (Zymo) with on-column DNase treatment per the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli Transformation Kit & Buffer Set (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... and PCR amplification protocol described above were applied to a larger set of varieties. PCR products (Fig. S1) were purified using the Zymoclean DNA recovery kit (Zymo Research) and submitted (without cloning into plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products with 150-400 bp were then cut and purified with 2% agarose gel and column (Zymo research, D4008). Finally ...
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bioRxiv - Microbiology 2023Quote: ... The negative controls had no or extremely low DNA concentrations to proceed to library preparation as determined by RT-PCR using the Femto Bacterial DNA Quantification Kit from Zymo and were therefore excluded from further analysis (n =10 ...
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bioRxiv - Bioengineering 2019Quote: ... coli Transformation Kit & Buffer Set (Zymo Research, USA). Transformed E ...
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bioRxiv - Bioengineering 2021Quote: ... coli Transformation Kit and Buffer Set (Zymo Research) and extracted by the Plasmid Plus Midi Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli Transformation Kit & Buffer Set (Zymo Research, USA) and used for bacterial transformation ...
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bioRxiv - Microbiology 2022Quote: ... coli Transformation and Buffer Set (Zymo Research, USA). Primers ...
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bioRxiv - Microbiology 2022Quote: ... coli Transformation and Buffer Set (Zymo Research, U.S.A). Transformants were selected and grown in phage-LB under a selective marker spectinomycin (50 μg/mL).
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bioRxiv - Microbiology 2022Quote: ... coli Transformation and Buffer Set (Zymo Research, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... coli Transformation Kit and Buffer Set (Zymo Research). We also prepared a H ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Cell Biology 2023Quote: ... Viral gene sequences in pPolI plasmids for all PR8 segments were amplified by PCR using corresponding primer pairs and were purified using ZYMO Research DNA cleaner and Concentrator-5 (ZYMO, D4014). Purified PCR products were in vitro transcribed using the T7 RiboMAX Express Large Scale RNA Production System (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA template for probe synthesis was generated by PCR amplification using T7 primers and purified using a DNA clean and concentrator kit (Zymo research). Antisense RNA probes were synthesized by in vitro transcription with either DIG-11-UTP (Sigma ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli Transformation Kit and Buffer Set (Zymo Research, T3002). For assemblies requiring maintenance of the ccdB cassette ...
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bioRxiv - Microbiology 2019Quote: ... free dNTPs and primers (Zymo Research) and subsequently used as templates for Sanger sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research ...
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bioRxiv - Synthetic Biology 2023Quote: ... Coli Transformation Kit and Buffer Set was purchased from Zymo research ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with the RNase-free DNase Set (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and treated with the RNase-free DNase Set (Zymo Research) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Genomics 2022Quote: ... DNAse on-column treatment was performed with the DNase I set (Zymo). RNA sample integrity for all samples was 10 ...
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bioRxiv - Genetics 2020Quote: ... using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research, Irvine, CA), S8 Table) ...
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bioRxiv - Neuroscience 2022Quote: ... and V3-V4 16S primers (Zymo Research, Irvine, CA). The sequencing library was prepared using real-time PCR ...
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bioRxiv - Microbiology 2022Quote: ... The size of 150-400 nt was cut and recovered by Zymo DNA agarose gel extraction kit (Zymo Research) ...
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bioRxiv - Genomics 2022Quote: ... and sizes 150-400 bp were sliced and extracted (Zymo research, D4008). Following steps were performed similar as previously described [23] ...
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bioRxiv - Microbiology 2020Quote: ... A total of 150 ng of the ZR small RNA ladder (Zymo Research) and 120 pmol of a homemade mix of synthetic RNA (MX with 31,29 ...
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bioRxiv - Plant Biology 2021Quote: ... Then 150 ng gDNA from each F2 plant was fragmented using dsDNA Shearase (Zymo Research) and used to generate one sequencing library per plant ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was purified by PCR purification kit (Zymo Research, D4013) and sent to Azenta Life Sciences for Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using Zymo PCR purification kit (Zymo Research) and directly transformed into Stellar™ chemical competent cells (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was synthesized from extracted RNA using ZymoScript RT PreMix kit (Zymo Research). 2 ng of the cDNA template was combined with 0.25 μM forward and reverse qPCR primers (Table S8) ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized by ZymoScript RT PreMix Kit (ZYMO Research, Cat#R3012). All gene expression results were expressed as an arbitrary unit (AU ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were PCR purified using the DNA Clean and Concentrator kit (Zymo Research), then transformed into One Shot TOP10 competent cells (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... PCR products were pooled and cleaned up with the Zymo PCR Cleanup Kit (Zymo) before performing 2% agarose gel separation and extraction with the Zymo Gel Extraction Kit following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified using the Zymo PCR purification kit® (Zymo Research©). Plasmid constructions were performed using In-fusion® HD-cloning kit (Takara© ...
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bioRxiv - Developmental Biology 2022Quote: ... The DNA digestion efficiency was monitored by using 100 ng 5-methylcytosine and 5-hydroxymethylcytosine DNA standard sets (Zymo Research) in parallel under identical conditions ...
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bioRxiv - Microbiology 2024Quote: ... Correct amplicons were isolated by Zymo PCR purification kit (Zymo Research). The PCR products were cloned into a PCR linearized pEcgRNA-guide plasmid to make the pEcgRNA-guide-RT plasmid using In-fusion® HD-cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted from 150 μl of supernatant using the Direct-zol RNA MiniPrep kit (Zymo, USA) according to the manufacturer’s instructions ...