Labshake search
Citations for Zymo Research :
651 - 700 of 744 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Cells were sorted by FACS as described above and RNA were extracted by using Quick- RNA Microprep Kit (Zymo Research, R1050) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The extracted DNA (100-1000 ng) from anal cells was bisulfite conversion by using EZ kit Gold Bisulfite Conversion Kit (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from cells following CTS or control treatments using the ZR-Duet™ DNA/RNA MiniPrep kit (Zymo D7001). RNA concentration and quality were measured using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cell lysates using the Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, USA) according to the mnufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Developmental Biology 2023Quote: Sw.71 cells pellets were lysed and RNA was extracted using the Zymo QuickRNA Microprep kit (Zymo Research, Irvine, CA USA). Whole placentas were equilaterally quartered and homogenized in RNA Lysis buffer and RNA was extracted using the Zymo QuickRNA miniprep kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... pEXG2 suicide vector containing 700-bp sequences of the flanking regions of the selected gene was directly inserted into competent SM10λpir (Mix&Go competent cells, Zymo Research Corporation) and subsequently selected on LB-Agar supplemented with 50 μg/mL kanamycin /15 μg/mL gentamicin ...
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bioRxiv - Genomics 2022Quote: ... At the indicated time points we harvested cells and extracted RNA using the Direct-zol-96 RNA kit (Zymo #11-331H). Samples were then sent to Novogene for bulk RNA-seq.
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 x 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Genetics 2023Quote: ... followed by resuspension of ~200k to 1 million cells in 300ul RNA Lysis Buffer from the Quick-RNA Miniprep Kit (Zymo R1055) and stored in −70deg ...
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bioRxiv - Microbiology 2024Quote: ... To determine the amount of virus that bound the cells viral RNA was isolated with RxnDirect-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturers’ protocol ...
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bioRxiv - Microbiology 2024Quote: The infected cells or homogenized lungs were lysed in Trizol to prepare total RNAs using Direct-Zol RNA Microprep kit (Zymo Research) with DNase treatment according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The bacmid encoding Halo-Ku80/Ku70 complex was obtained by electroporating pAM086 into DH10EMBacY electro-competent cells and purified using ZR BAC DNA miniprep kit (Zymo Research).
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bioRxiv - Bioengineering 2024Quote: Up to 1 × 108 yeast cells from a growing culture were taken to perform a zymoprep using the ZymoprepII kit (D2004, Zymo Research). The TRACeR sequences were amplified from the extracted plasmids and the adapter sequences were added by PCR ...
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bioRxiv - Genetics 2024Quote: ... DNA from a minimum of 1 e-6 cells from each fraction was isolated using the YeaStar Genomic DNA Kit (Zymo Research). DNA was fragmented by sonication and sequenced.
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bioRxiv - Genomics 2024Quote: ... secondary plant compounds hampering the activity of the pores in the Oxford Nanopore Technologies (ONT) flow cells were removed with the Quick-DNA HMW MagBead Kit (Zymo Research). Sequencing libraries were prepared with the LSK-109 ligation library preparation kit and sequencing was performed on MinION R.9.4.1 flow cells ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was isolated from previously frozen cell pellets and bisulfite converted using the EZ DNA methylation-direct kit (Zymo Research). Two PCRs were performed with the PyroMark PCR kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: RNA and DNA were extracted from the same frozen cell pellets using the ZR-Duet DNA/RNA MiniPrep kit (D7001, Zymo, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Genomic DNA was extracted from cells or tumor tissues and subjected to bisulfite treatment using an EZ DNA Methylation-Gold Kit (ZYMO RESEARCH, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and genomic DNA (gDNA) of surviving cells was isolated using a genomic DNA cleanup kit according to manufacturer instructions (Zymo Research, D4065). For Illumina sequencing and screening analysis ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA from ∼106 parental or sorted CEM/R5 cells or CD4 lymphocytes was purified using Quick-gDNA MiniPrep Kit (Zymo Research, USA). The CXCR4 target regions were PCR-amplified using 200 ng of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from yeast cells grown into SD-Trp-Leu to mid-log phase was isolated with YeaStar RNA Kit (Zymo Research R1002). RNA was treated with 0.02 units/µl DNase I (NEB M0303 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.
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bioRxiv - Cancer Biology 2022Quote: ... and from cell lines using and Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA, USA). ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
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bioRxiv - Bioengineering 2021Quote: ... control targeted cells (cells that underwent CRISPR/Cas9-mediated targeting and HDR of an expression cassette at a non-GSH locus) and two independent cultures of untargeted cells using Direct-zolTm RNA Miniprep kit (Zymo Research, ZYR.R2052). 1μg of RNA was converted into cDNA with Superscript IV Vilo MM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from individual eGFP+ cell lines was extracted from 96 well-plates using Quick-DNA Universal 96 Kit (Zymo Research, D4071) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 250 μl of this cell suspension was added to 250 μl of lysis buffer in ZR BashingBead™ lysis tubes (Zymo Research) containing 0.1 mm and 0.5 mm beads ...
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bioRxiv - Microbiology 2022Quote: ... gondii infected-PC12 cells that were harvested on day five post-infection using the Direct-zol RNA MiniPrep Plus kit (Zymo Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from HBEC controls and various HBEC derivative cells (three different clones for each group) using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). DNA samples were submitted to the Van Andel Institute’s Genomics Core (Controls ...
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: RNA from HEK Flp-In™ T-REx™ 293 and A549 cells was extracted using Zymo III columns (Zymo, Freiburg, Germany). Reverse transcription was performed using random hexamer primers (IDT ...