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Citations for Zymo Research :
501 - 550 of 744 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the pelleted cells and debris were resuspended in 500 μL of 2x DNA/RNA Shield (Zymo Research (Irvine, CA) and stored at –20°C ...
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bioRxiv - Biophysics 2022Quote: ... Plasmids used for transient expression in HEK293T cells were prepared using the Zymopure Midiprep Kit (Zymo Research, Irvine, CA).
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bioRxiv - Cell Biology 2021Quote: RNA was prepared by collecting cells in TRIzol reagent and isolated using the Direct-zol RNA Kit (Zymo Research) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... RNA extraction for cell samples was performed using TRI Reagent with a Direct-zol-96 RNA Kit (Zymo Research), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Total RNA samples were prepared from cells/organoids using TRIzol and Direct-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Bulk total RNA was prepared from ~1.5*105 cells using the Direct-zol RNA MiniPrep kit (R2050, Zymo Research) according to the manufacturer’s instructions and stored in − 80 °C ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Cells were washed in cold PBS then total RNA isolated using quick-RNA extraction mini prep kit (Zymo research) according to the manufacturer’s instructions before quantification on Nanodrop ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed in Trizol and total RNA was extracted using the Direct-zol kit (Zymo Research, CA USA). On column DNA digestion was also performed with DNAse treatment ...
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bioRxiv - Systems Biology 2022Quote: ... with the following differences: Total RNA was extracted from cells using the Direct-zol RNA Miniprep kit (ZYMO Research). RNA samples (0.5 µg) ...
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bioRxiv - Microbiology 2022Quote: ... (Hayashi et al., 2009): DL70 cells were made chemically competent (Frozen-EZ Yeast Transformation II Kit, Zymo Research, #T2001) and were transformed with a linear DNA fragment consisting of the mScarlet-I sequence and a hygromycin resistance cassette flanked by 500-600 bp of homology arms on both sides for homologous recombination into the genome downstream of the native sad1 gene ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: Total RNA was isolated from 2-3 million HAP1 cells using the Direct-zol-96 RNA kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Competent cells for each yeast strain were prepared using the Frozen-EZ Yeast Transformation II Kit™ (Zymo Research) according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2020Quote: ... Cells were harvested 36h after transfection and total RNA was extracted using Quick-RNA MiniPrep Plus kit (ZYMO Research). cDNA was transcribed using 750ng of isolated RNA SuperScript™ III Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from sorted memory CD4 T cells using Quick-DNA Microprep kit (Zymo Research, Irvine, CA, USA). ImmunoSEQ hsTCRB sequencing kit (Adaptive Biotechnologies ...
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bioRxiv - Genomics 2021Quote: Cells were lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo research). 600ng of template RNA was retrotranscribed into cDNA using RevertAid first strand cDNA synthesis kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in Trizol and total RNA was extracted using the Direct-zol kit (Zymo Research, CA USA). On column DNA digestion was also performed with DNAse treatment ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated from cultured B cells at indicated timepoints using the Quick-RNA Microprep Kit (Zymo Research). cDNA was synthesized with the RevertAid RT Kit (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... Complementary plasmids were co-transformed into competent MC1061ΔpspF cells carrying the reporter plasmid pLY54 (prepared using the Mix & Go E. coli Transformation Kit from Zymo Research ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from cells using TRIZOL and purified using the Direct-zol RNA MiniPrep Plus kit (Zymo, R2072). The concentration and purity of RNA extracts were determined by Nanodrop ...
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bioRxiv - Cell Biology 2022Quote: ... then cell body and neurite samples were processed for RNA isolation with a Quick-RNA Microprep Kit (Zymo, R1050). Following isolation ...
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bioRxiv - Developmental Biology 2022Quote: Cells were lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo research). 600ng of template RNA was retrotranscribed into cDNA using RevertAid first strand cDNA synthesis kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: Total RNA was isolated from ∼1.5 X 107 yeast cells using Quick-RNA Fungal/Bacterial Microprep Kit (Zymo Research). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from the cultured cells using TRI reagent (Bio-lab) and an RNA extraction kit (ZYMO) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 3×105 cells per replicate using Direct-Zol RNA Miniprep Kit (Zymo Research, USA) according to the manufacturer’s protocol and shipped in dry ice to the MDC/BIH Genomic Core Facility for quality assessment using TapeStation (Agilent Technologies) ...
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bioRxiv - Bioengineering 2022Quote: DNA of the active population was extracted from yeast cells using Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research) and amplified in two steps ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and prepared for total RNA purification using the Quick-DNA/RNA Viral MagBead kit from Zymo Research and an Agilent Bravo NGS A liquid handler(32) ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was harvested from cells 24-hours post-transfection using the Direct-zol RNA Miniprep kits (Zymo Research). Each in vivo splicing assay was performed a minimum of three times.
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bioRxiv - Biochemistry 2023Quote: ... These cells were cultured in LB media and plasmids were purified using ZymoPURE II Plasmid Midiprep kit (Zymo Research). Each plasmid was then digested with EcoRI-HF and BamHI-HF (NEB ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from the sorted CD8+ T cells using the Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were snap-frozen on liquid N2 for RNA extraction performed using Direct-zol RNA MicroPrep kit (Zymo Research).
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bioRxiv - Genetics 2023Quote: ... strains (OD600= 0.3 - 0.5; ∼107 cells/ml) in YPD supplemented with 0.1 μg/ml a- factor (Zymo Research, Y1004) for 3 h 30 min (yDT67 ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA in cell lysates was extracted using a Quick-RNA extraction miniprep kit (# R1055 Zymo Research, CA, USA) following the manufacturer’s instructions and RNA concentration determined by the nanodrop machine (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from the sorted CD8+ T cells using the Direct-zol RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Cells were washed twice in cold PBS and lysed in 400 μl of RNA/DNA lysis buffer (Zymo Research). Total cellular RNA was extracted according to the manufacturer’s protocol for Quick-DNA/RNA minipreps ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified for transfection into mammalian cell lines using a ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA (gDNA) was purified from ES cells and tail clips using the Quick-DNA Miniprep Kit (Zymo Research). Initial validation of target insertion was performed with primers designed to amplify across the 5’ and 3’ integration sites (Supplementary data 1-4) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from transduced HEK 293 cells using the Zymo Quick-RNA Microprep Kit (Zymo Research, #R1050). Reverse transcription was performed with Invitrogen’s SuperScript IV VILO Master Mix with ezDNAse enzyme (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Systems Biology 2023Quote: ... We thawed the cell pellets on ice and then resuspended with 400 µL RNA binding buffer (Zymo, #R1013-2). The mixtures were then transferred to 2 mL screw cap tubes with zirconia beads inside ...
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bioRxiv - Cancer Biology 2023Quote: RNAs from respective cell lines were isolated and purified via the Zymo Quick-RNA microprep kit (Zymo Research, #R1058). 1000 ng of DNAse-treated RNA was converted to cDNA using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Immunology 2023Quote: ... cells and tissue were harvested in TRIzol and RNA was isolated using Direct-zol RNA Miniprep kits (Zymo Research) with 1 h DNase treatment ...
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bioRxiv - Bioengineering 2024Quote: ... The cell samples were lysed with Trizol (Life-science) and RNA purification was done with Zymo kit (R1050, Zymo). RNA quantification was assessed with a Nano Drop 8000 Spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA of the snap frozen cells was extracted using the Quick-DNA Midiprep Plus Kit (Zymo Research, D4075). sgRNA amplification was performed following a previously published protocol(49) ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from transduced HOS cells using a Quick-DNA Miniprep kit (Zymo Research, Irvine, CA, USA). Vector titers were determined by calculating the ratio between the copies of HIV psi and every two copies of RPP30 via QX200 digital droplet PCR system (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... At least four million IL-1βlow and IL-1βhigh cells were collected for genomic DNA extraction using Quick-DNA FFPE Kit (Zymo Research). PCR reactions containing up to 10 μg genomic DNA in each reaction were performed using ExTaq DNA Polymerase with primers to amplify the sgRNAs ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified from these cell lysates using the standard protocol in the DNA extraction kits (Zymo Research #D3010), except that DNA was resuspended in sterile water in the final step ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from total cell lysates directly from beads after IP using TRIzol RNA Miniprep Plus (Zymo Research). First ...
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bioRxiv - Immunology 2024Quote: Bisulfite modification of genomic DNA from FACS purified cells was performed using the EZ DNA methylation kit (Zymo Research). For methylation analysis on Ifng gene promoter CpG sites ...