Labshake search
Citations for Zymo Research :
201 - 250 of 303 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
-
bioRxiv - Systems Biology 2021Quote: ... at which point a 200 µL aliquot was removed and preserved in 1 mL of DNA/RNA Shield (Zymo Research) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were diluted to 1 nM using the Select-a-Size DNA Clean and Concentrator MagBead Kit (Zymo Research) and pooled for further sequencing on the Illumina MiniSeq platform ...
-
bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
-
bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
-
bioRxiv - Biophysics 2021Quote: ... Sufficient amounts of DNA were obtained growing 1 L of transfected XL1 Blue Escherichia coli cells overnight and performing a gigaprep (Zymo). For protein expression ...
-
bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
-
bioRxiv - Genomics 2021Quote: Total RNA was purified in duplicate from 1 million frozen cells using the Direct-zol RNA MiniPrep kit from Zymo Research (#R2050) ...
-
bioRxiv - Genetics 2020Quote: ... total RNA from approximately 1 × 108 cells was extracted using the Quick-RNA Fungal/Bacterial Miniprep (Zymo Research, Irvine, CA). For each sample ...
-
bioRxiv - Microbiology 2022Quote: ... Swabs were vortexed for 10 seconds in 300 μL of 1 × DNA/RNA Shield™ (Zymo Research, Cat. No. R1100) and centrifuged for 5 minutes at 5,000 × g ...
-
Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were lysed and DNA was extracted at days 1 and 5 utilizing the Quick-DNA/RNA Miniprep (Zymo Research). Viral genomes were quantified using a standard curve and normalized to RNAseP.
-
bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
-
bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
-
bioRxiv - Biochemistry 2023Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
-
bioRxiv - Synthetic Biology 2023Quote: ... except the first step started from yeast plasmid prepared from 5 × 107 to 1 × 108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
-
bioRxiv - Genomics 2023Quote: ... the cells were pelleted and resuspended at 1x106 cells per 300 ul of RNA lysis buffer (Zymo, #R1060-1-100). Cells were pipette mixed and frozen at -80 until RNA isolation was performed ...
-
bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was transferred to a fresh tube and 1 volume of 100% ethanol was added and mixed thoroughly before binding to an RNA Clean & Concentrator (Zymo). RNA was DNase I digested on-column (Zymo) ...
-
bioRxiv - Genomics 2024Quote: ... tagmentation was performed at 55°C for 5 min after the addition of 1/50uL of N7-adaptor loaded Tn5 and subsequent quenching with DNA binding buffer (Zymo) for 5 min at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... a small piece of gel (∼100 μl volume) was dissolved in 400 μl TRI-reagent (Zymo Research, R2050-1-200) and the RNA was purified using an RNA isolation kit (Zymo Research ...
-
bioRxiv - Plant Biology 2019Quote: ... at 50°C for 1 h and RNA was extracted from input and pellet fractions using TRIzol and RNA Clean and Concentrator Columns (Zymo Research) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... DNA was then extracted from approximately 1 ml of the red blood cell pellets using Zymo Bead Genomic DNA kit (Zymo Research) following the manufacturer’s procedures ...
-
bioRxiv - Genomics 2020Quote: ... We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200) the resulting library.
-
bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... fine forceps were used to manually remove each neuron. Identified neurons (Fig. 1) were immediately placed in a cryogenic microcentrifuge tube containing 400 µL lysis buffer (Zymo Research) and stored at −80°C until RNA extraction ...
-
bioRxiv - Plant Biology 2019Quote: ... RNA was isolated from Tak-1 and Tak-2 whole-plant material using the Direct-Zol RNA Mini-Preparation Kit (Zymo Research). cDNA synthesis was carried out using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... The Variovorax CL14 genes contained in Vectors 1 and 2 were inferred by isolating plasmid from these clones using the ZymoPURE II Plasmid Midiprep Kit (Zymo Research) and Sanger sequencing the insert ends using primers JMC247 and JMC270 (Supplementary Table 19) ...
-
bioRxiv - Biochemistry 2021Quote: ... The PCR products were subjected to 1% agarose gel electrophoresis and they were purified using a Zymogen gel DNA recovery kit (Zymo Research). The assembly reactions were transformed to chemically competent E ...
-
bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from GBM-DCs and TRA-1-81+/SSEA4+ ic-GSCs using the Quick-DNA Microprep Kit (D-3020, Zymo Research). Bisulfite conversion ...
-
bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... genomic DNA was extracted from 1 ml breastmilk using Quick-DNA Fungal/Bacterial extraction kit following the manufacturer’s instructions (Zymo Research, USA). Extraction kits were purchased separately for Batch 1 and Batch 2 ...
-
bioRxiv - Plant Biology 2022Quote: ... A final concentration of 0.1 ng/µL was employed for both the Femto Fungal DNA Quantification Kit and Femto Bacterial DNA Quantification Kit (Zymo Research) according to the manufacturer protocol ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
-
bioRxiv - Molecular Biology 2019Quote: Cells were infected with 1 MOI of TB40/EGFP and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0-72 hpi ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
-
bioRxiv - Plant Biology 2020Quote: ... ToMVMP-ToBRFV or ToMVMP-ToBRFV(1-216) were linearized with SmaI and cleaned by Gel extraction kit (Zymo Research, ZR-D4002). In vitro transcription using the mMESSAGE mMACHINE™ T7 kit (Invitrogen by Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... 100 mg of thawed cell pellets were washed three times in 1 mL deionized water and extracted using Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research). A total of 2.4 μg of purified gDNA was fragmented by sonication to average size of ~350 bp in four separate aliquots using a Diagenode Picoruptor ...
-
bioRxiv - Genomics 2022Quote: ... A 2-cm-long piece of each cotton strip was cut off and transferred into a ZR BashingBead Lysis Tube (0.1 & 0.5 mm) pre-filled with 1 mL of DNA/RNAShield (Zymo Research, Freiburg, Germany) using sterile laboratory gloves ...
-
bioRxiv - Microbiology 2022Quote: ~1 kb products from the Pan-Entamoeba PCR above were gel purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research) and submitted to the UW-Madison Biotechnology Center for Sanger Sequencing using the amplification primers described above ...
-
bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
-
bioRxiv - Microbiology 2023Quote: Cells were infected with 1 MOI of TB40/EGFP and DNA and RNA was isolated using Quick-DNA/RNA miniprep kit (Zymo Research) from 0–72 hpi ...
-
bioRxiv - Genomics 2023Quote: Total RNA (∼1 µg) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
-
bioRxiv - Molecular Biology 2023Quote: ... nojo-1 and nojo-2 complete roots of plants growing 12 DAS using Quick-RNA Miniprep (Zymo Research, Irvine, CA, USA). Complementary DNA was generated using the SuperScriptTMII Reverse Transcriptase (InvitrogenTM ...
-
bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
-
bioRxiv - Genomics 2023Quote: ... RNA was extracted from each well of a 6-well plate containing ES or DE cells using 1 mL Tripure and the Direct-Zol RNA Extraction kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Constructs were circularized with vector backbone by Gibson assembly and introduced to XL-1 Blue Mix and Go chemical competent cells (Zymo Research), miniprepped (Qiagen) ...
-
bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
-
bioRxiv - Genomics 2023Quote: gDNA was isolated from ∼25 mg of frozen tissue or ∼1 x 106 HepG2 cells suspended in 200 µL of PBS using the Quick-DNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol with an overnight (for frozen tissue ...