Labshake search
Citations for Zymo Research :
1 - 50 of 303 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
-
bioRxiv - Plant Biology 2021Quote: ... DNAs from the immuno-complex was purified by kit (Zymo Research, D5205). The half of the purified DNA was subjected to library preparation using ACCEL-NGS® 2S PLUS DNA LIBRARY KIT with 2S Set A MID Indexing Kit (Swift bioscience ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted from CEM-SS or Tet-inducible HTLV-1 Tax CEM-SS cells that were infected with DNaseI-treated WT or IN- HIV-1 at 2dpi using a DNA Miniprep Plus kit (Zymo Research). Purified DNA was then digested with MscI ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: DNA for HLA typing was isolated from PBMCs using the Quick-DNA/RNA™ Microprep Plus Kit (Zymo Research, Irvine, CA, USA). High throughput-HLA typing was performed by the Institute of Transplantation Diagnostics and Cell Therapeutics (ITZ) ...
-
bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: RNA from Anabaena WT was isolated using the Direct-zol™ RNA MiniPrep Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from OSC:eFH-piwi and wild type (wt) OSC using gDNA kit (Zymo Research). PCR amplification was done using Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Genetics 2019Quote: ... Total RNA was extracted from WT and Δprmt7 input samples using Direct-zol RNA Miniprep (Zymo Research). cDNA was synthesized from 2 μg of total RNA or 100-400 ng of RIP RNA using the SuperScript IV Reverse Transcriptase (Invitrogen) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The bacmid encoding Halo-Ku80/Ku70 complex was obtained by electroporating pAM086 into DH10EMBacY electro-competent cells and purified using ZR BAC DNA miniprep kit (Zymo Research).
-
bioRxiv - Cell Biology 2023Quote: RNA was prepared from WT and LMNA KO MEFs using the Direct-zolTM RNA MiniPrep kit from Zymo Research (#R2050) ...
-
bioRxiv - Immunology 2020Quote: Total cellular RNA from WT and POLG BMDMs and PerMacs was prepared using Quick-RNA microprep kit (Zymo Research) and used for the Next-generation RNA sequencing procedure at Texas A&M University Bioinformatics Core ...
-
bioRxiv - Developmental Biology 2020Quote: RNA for WT and LSD1KO crypts was isolated by sorting IECs (DAPI-, Epcam+) in 2x RNA shield buffer (Zymo) and RNA isolation using the Quick-RNA Micro prep kit (Zymo) ...
-
bioRxiv - Genetics 2020Quote: ... Genomic DNA was purified from WT and Chr4-cl KO ES cells using the Quick-gDNA purification kit (Zymo Research) and bisulfite-converted with the NEXTflex™ Bisulfite-Seq Kit (Bio Scientific ...
-
bioRxiv - Synthetic Biology 2019Quote: Genomic DNA was prepared from both the WT strain as well as the different melamine-utilizing strains by using the Quick-DNA Fungal/Bacterial Kit (Zymo Research). Library preparation was performed according to Illumina’s TruSeq Nano DNA Sample Preparation protocol ...
-
bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from OSC:eFH-piwi and wild type (WT) OSC using Trizol and Direct-zol RNA MiniPrep kit (Zymo Research, R2051). Complementary DNA (cDNA ...
-
bioRxiv - Developmental Biology 2019Quote: Sodium bisulfite modification of genomic DNA (500 ng) of WT and Etv5 KO mESCs was performed according to the instruction of EZ DNA Methylation-DirectTM Kit (Zymo Research). Methylation specific PCR for a fragment of Fgfr2 promoter was carried out as we previously described (Zhang et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... LPI-5 and LPI-15 were collected from three greenhouse-grown WT plants (∼5 ft in height) for RNA extraction using Direct-zol RNA MiniPrep kit (Zymo Research) with Plant RNA Purification Reagent (Invitrogen) ...
-
bioRxiv - Genomics 2024Quote: ... we extracted total RNA from each line (5 wt lines and 5 +0A lines) using the Zymo Direct-Zol RNA extraction kit (Zymo Research). This RNA was immediately reverse transcribed using Superscript III reverse transcriptase (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
-
bioRxiv - Plant Biology 2019Quote: Total RNA from 4 weeks old Arabidopsis thaliana WT Col-0 and mta mutant plants was isolated using Direct-zol™ RNA kit (Zymo Research). RNA was quantified by Qubit RNA Assay Kit (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
-
bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
-
bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
-
bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from the isolated meiocytes (WT, clsy3, and clsy4) or tapetal cells using Direct-zol RNA Kit (Zymo Research, cat. no. R2061). Sequencing libraries were constructed using the RealSeq-Biofluids NGS Library Preparation Kit for miRNAs and small RNAs (BioCat ...
-
bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein degradation was performed by adding Proteinase K (Zymo) and Qiagen Protease (Qiagen) ...
-
bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
-
bioRxiv - Cell Biology 2023Quote: ... ChIP-DNA was prepared using ZymoMag Protein A beads (Zymo Research). Input samples were also prepared using 10% of chromatin samples ...
-
bioRxiv - Microbiology 2022Quote: For human OE and OB RNA was extracted from 10mg of tissue per sample using Direct-zol RNA kit from Zymo Research (Catalog #R2052) ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
-
bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
-
bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
-
bioRxiv - Immunology 2022Quote: pAd/S1RS09cg proteins were amplified and purified using ZymoPURE II plasmid maxiprep kit (Zymo Research). For Expi293 cell transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...
-
bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
-
bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
-
bioRxiv - Biochemistry 2023Quote: ... and the DNAs were purified away from protein using a DNA Clean & Concentrator kit (Zymo Research). Both 5’ ends of 1 pmole of the CRISPR leader and array fragments were then labelled with 32P ...
-
bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... Then the nucleic-acid bound protein isolation was performed using buffers from the Quick-RNA Miniprep Kit (Zymo Research) and the Zymo-Spin IIICG Column (Zymo Research) ...
-
bioRxiv - Cell Biology 2023Quote: ... Endogenous RNA cross-linked to bait protein was recovered from the beads using the QuickRNA Microprep Kit (Zymo Research). Complementary DNA was synthesized using the SuperScript III First-Strand Synthesis kit (Invitrogen ...