Labshake search
Citations for Zymo Research :
151 - 200 of 252 citations for 6 chloro 9H 3 C methyl β D ribofuranosyl purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the 100–400nt region was cut and dissolved in 1X TEN250 buffer (1X TE 7.4, 0.25M NaCl) at 80°C for 30min followed by column purification (Zymo research, R1016). For the HEK293T libraries ...
-
bioRxiv - Microbiology 2023Quote: RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
-
bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
-
bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
-
bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
-
bioRxiv - Genetics 2019Quote: 25-30 eyes were dissected from both WT and pitx2ΔCE5-7 eye zebrafish embryos at 3 dpf in Ringer buffer and genomic DNA was purified using Quick-DNA microprep Plus kit (ZYMO Research). Eyes were resuspended in Solid Tissue buffer containing Proteinase K and incubated at 55°C for 6 hours ...
-
bioRxiv - Microbiology 2019Quote: ... at 48 hpe cells were washed 3 times with PBS to eliminate extracellular virus and total RNA was extracted using Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
-
bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
-
bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
-
bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
-
bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
-
bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
-
bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
-
bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
-
bioRxiv - Developmental Biology 2021Quote: ... The reactions were incubated for 30 min at 37°C and immediately purified using ZYMO RNA Clean & Concentrator (ZYMO RESEARCH, D7011). The purified RNA samples were eluted in 20 μl of nuclease free water ...
-
bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
-
bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2019Quote: ... Digests were heat inactivated for 20 minutes at 80°C then column cleaned using the DNA Clean and Concentrator kit (Zymo Research). Dilute ligations (100-500 ng DNA ...
-
bioRxiv - Biochemistry 2019Quote: ... Beads for protein analysis were incubated with Laemmli buffer and heated at 95°C for 10 min and beads for RNA analysis were exposed to TRI reagent (Zymo Research) for RNA extraction using Direct-Zol (Zymo Research ...
-
bioRxiv - Biochemistry 2019Quote: ... the DNA samples used for 450k analysis were digested overnight at 37°C using BamHI and EcoRI and then bisulfite converted using EZ DNA Methylation-Lightning Kit (Zymo Research) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The intermediate RNA products were removed with NaOH-EDTA solution in 95 °C for 10 minutes and the single strand DNA oligo probes were purified via DNA clean & concentrator-25 (D4033, Zymo Research). The ssDNA probes were either directly used for Oligopaint FISH or store in aliquots at −80°C.
-
bioRxiv - Cancer Biology 2020Quote: ... Transposition was carried out at 37°C for 30 minutes followed by DNA purification with DNA Clean and Concentrator-25 (Zymo Research) according to manufacturer’s recommendation ...
-
bioRxiv - Biochemistry 2019Quote: ... Elutions were subsequently de-crosslinked overnight at 65 °C and DNA was purified using ChIP DNA Clean and Concentrator (Zymo Research) and DNA was used for qPCR ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
-
bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
-
bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
-
bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
-
bioRxiv - Microbiology 2023Quote: ... The reaction was incubated at 32°C for 4 hours before being treated with DNase for 20 minutes at 37°C then purified using an RNA clean and concentrate kit (Zymo Research). The RNA quality was checked using a MOPS/formaldehyde agarose gel electrophoresis.
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were either resuspended in 30 µL 100% RNALater and stored at -70°C or resuspended in 100 µL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research).
-
bioRxiv - Immunology 2023Quote: ... incubated for 15 min at room temperature and either frozen at -80°C or directly followed by RNA isolation using a Direct-zol RNA microprep kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: Two hundred and fifty nanograms of linker-ligated genomic DNA were subjected to sodium bisulfite conversion for 210 min at 64°C using the EZ DNA Methylation Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
-
bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
-
Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...