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Citations for Zymo Research :
1 - 50 of 252 citations for 6 chloro 9H 3 C methyl β D ribofuranosyl purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Genomics 2024Quote: Pico Methyl-Seq Library Prep Kit (Zymo research) was used for making WGBS libraries basically as manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was hydrolysed for 6 h at 37°C using DNA degradase plus (Zymo Research). Mass spectrometry quantification was performed as previously described36 in the Babraham Institute mass spectrometry facility using three individual E18.5 wild-type and three individual E18.5 Tet3 knockout embryos.
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Synthetic Biology 2020Quote: ... were pretreated with a solution of β-mercaptoethanol plus 60 mM EDTA and then incubated with 6 U/mL zymolyase (Zymo Research) in Buffer I to remove the cell wall ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated at 65 °C for 6 hours and extracted with ChIP DNA Clean&Concentrator kit (Zymo Research, USA).
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bioRxiv - Molecular Biology 2019Quote: PBAT libraries were prepared using Pico Methyl-Seq Library Prep Kits (Zymo D5456). Sperm and egg isolates of approximately 100 cells each were diluted in 200 ul of 10 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... libraries were prepared with the Pico Methyl-Seq prep kit (Zymo Research #D5456) and sequenced by the Next Generation Sequencing Facility (Vienna BioCenter Core Facility ...
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bioRxiv - Bioengineering 2019Quote: The 3-D centrifuge was used to perform DNA extractions using the Quick-DNA Miniprep Plus kit (Zymo Research) according to the protocol ...
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bioRxiv - Plant Biology 2022Quote: ... libraries were prepared with the Pico Methyl-Seq Library Prep Kit (Zymo Research #D5456) and sequenced by the next Generation Sequencing Facility (Vienna BioCenter Core Facilities) ...
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bioRxiv - Cancer Biology 2022Quote: ... WGBS libraries were generated using the Pico Methyl-Seq Library Prep Kit (Zymo Research) with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... WGBS libraries were generated using the Pico Methyl-Seq Library Prep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... amplification and library construction procedures were performed with Pico Methyl-SeqTM Library Prep Kit (Zymo Research) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Libraries for Illumina-based sequencing were prepared with the Pico Methyl-Seq Library Prep Kit (Zymo Research) using Illumina TruSeq DNA methylation indices ...
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bioRxiv - Neuroscience 2023Quote: Sorted nuclei DNA on beads were processed by Pico Methyl-Seq Library Prep Kit (Zymo Research, D5455) to generate bisulfite sequencing libraries ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Neuroscience 2019Quote: ... Methylation-sensitive restriction enzyme-dependent methylation analysis was performed using OneStep q-Methyl Kit (Zymo, Irvine, CA, USA) following the instructions given in the manual using 20ng DNA for each sample ...
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bioRxiv - Plant Biology 2021Quote: ... Bisulfite converted DNA was used to build libraries with the Pico Methyl-Seq library kit (Zymo Research, D5455). 7 cycles of amplification was used for library construction ...
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bioRxiv - Genomics 2020Quote: ... We created libraries from bisulfite-converted single stranded DNA using the Pico Methyl-Seq Library Prep Kit (Zymo Research), which were then pooled for sequencing on an Illumina NextSeq 500 instrument using the NextSeq 500/550 V2 High Output reagent kit (1 × 75 cycles ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Neuroscience 2019Quote: ... The whole genome bisulfite sequencing (WGBS) libraries were prepared using Pico Methyl-seq library prep kit (Cat# D5456, Zymo research). Paired-end ...
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bioRxiv - Immunology 2020Quote: ... WGBS libraries were prepared using 100 ng of DNA using the Pico Methyl-Seq™ Library Prep Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries were prepared genomic DNA (10 ng) using a Pico Methyl-Seq Library Prep Kit (ZYMO, catalog # D5456). MQ beads (Epigentik ...
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Genetics 2020Quote: ... We then prepared whole-genome bisulphite sequencing libraries from each DNA sample using the Pico Methyl-Seq Library Prep Kit (Zymo Research), according to the manufacturer’s protocol (see Supplementary Table 1 for detailed sample metadata and sequence accession codes) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... 6) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA), by the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... pH 7.4) in the presence of 0.1% β-mercaptoethanol and 25 Units of Zymolase (Zymo Research). Spheroblasts were lysed by centrifugation in Qiashredder columns and total RNA was isolated using the RNEasy Mini Plus kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Microbiology 2021Quote: ... Exceptions to these protocols were as follows: (i) RNA purification after acylation was performed using RNA C&C columns (Zymo Research), rather than ethanol precipitation ...
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bioRxiv - Systems Biology 2023Quote: ... 600 µL DNA/RNA wash buffer (Zymo, #D7010-3), and 400 µL DNA/RNA wash buffer ...
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bioRxiv - Genomics 2024Quote: Neutralization Buffer (Zymo Research, cat. no. D4027-3-50)
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bioRxiv - Molecular Biology 2021Quote: ... C-SCCE0625), and ScriptCap 2′-O-Methyltransferase (Cellscript, C- SCMT0625) and purified by a Direct-zol RNA Microprep Kit (Zymo Research, D2060). The radiolabeled TOP RNAs (4 pmol ...
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bioRxiv - Genomics 2022Quote: ... at 37°C for 1h and purified by Zymo columns ...
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bioRxiv - Biochemistry 2023Quote: ... assembly products were purified and eluted in 6 μL water (Zymo Clean and Concentrate).
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bioRxiv - Molecular Biology 2023Quote: ... The products were purified and eluted in 6 μL water (Zymo Clean and Concentrate). Using the same procedure as above ...
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bioRxiv - Molecular Biology 2022Quote: ... Viral D NA was extracted using a Quick-DNA™ Viral Kit (ZYMO Research). The sta ndard plasmid pI-MVC (Sun et al. ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from 14 d-old seedlings using TRI Reagent (Zymo Research) according to the supplier’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the products were individually purified and eluted in 6 μL water (Zymo Clean and Concentrate). NEB-10β E ...
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bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
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bioRxiv - Molecular Biology 2019Quote: Plasmids were extracted from yeast with Yeast Plasmid Miniprep 1 kit (Zymo Research, D-2001). Library fragments were amplified in 17 PCR cycles using primers specific to DNA sequences that flank Aβ-DHFR in p416 ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembly products were then cleaned and eluted in 6 μL water (Zymo Clean and Concentrate). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... and frozen at −80°C in 500 ul TRI-Reagent (Zymo). Frozen worms were thawed on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was extracted from 6-14 dpf embryos (after yolk removal) using the DNA miniprep kit (Zymo). PCR products were amplified with Phusion (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... ∼4-6×109 cells were concentrated and suspended in 500 μl of DNA/RNA Shield (Zymo Research) in 2 mL screw cap tubes ...
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bioRxiv - Biophysics 2023Quote: ... The reactions were quenched with β-mercaptoethanol (50% by volume) followed by column purification (Oligo Clean & Concentrator, Zymo Research #D4060, here and below). The adenylated 18 nt unique molecular indicator (UMI ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and eluted in 10 μL prewarmed 65°C provided elution buffer (Zymo). Six μL of each were then combined and sequenced off the U2 region via Illumina amplicon sequencing ...