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Citations for Zymo Research :
351 - 400 of 484 citations for 7 nitro 3 oxido 6 4 phenylpiperazin 1 yl 2 1 3 benzoxadiazol 3 ium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Genomic DNA (1 μg) was treated with sodium bisulfite using the EZ DNA Methylation Gold Kit per manufacturer’s instructions (Zymo Research; Irvina, CA). Bisulfite converted DNA (∼20 ng ...
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bioRxiv - Immunology 2021Quote: ... PCR products were subjected to 1% agarose DNA gel electrophoresis and gel-purified with Zymoclean™ Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: DNA bisulfite conversion was performed starting from a maximum of 1 × 105 pelleted cells per sample using the EZ DNA Methylation-Direct kit (Zymo Research #D5021), and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were grown in Sc-Ura medium for 1 d and plasmid DNA was purified using Zymoprep II Yeast plasmid Miniprep kit (Zymo Research, CA). Plasmids were then transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... The adapter-ligated DNA molecules were purified using 1× Agencourt AMPure XP magnetic beads and were subjected to bisulfite conversion using ZYMO EZ DNA Methylation-Gold Kit (Zymo, Cat. #D5005). Libraries were then amplified by PCR using 20 μL of bisulfite-converted DNA molecules ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Microbiology 2024Quote: ... The amplified promoter and coding PIGJ DNA sequence were first purified from 1% agarose gels using the ZymocleanTM Gel DNA recovery kit (Zymo Research, #D4007) and assembled in frame into the digested pLIC-HA -DHFR-TS plasmid in frame with the 3x HA tag using the NEBuilder HiFi DNA Assembly cloning Kit (NEB ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Genetics 2023Quote: ... individual array bearing worms were isolated and lysed in 4µl of EB (Zymo, Cat. No.: D3004-4-16) buffer with 1mg/ml proteinase K (NEB) ...
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bioRxiv - Genomics 2023Quote: ... worms were thawed at 4°C and subsequently preserved in DNA/RNA Shield (Zymo Research, Irvine, CA, USA). Adults of T ...
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bioRxiv - Microbiology 2024Quote: ... hydrophila MAH-4 was pelleted and genomic DNA obtained using Zymo DNA Miniprep (Zymo Research, Irvine, CA, USA). Long reads and short paired end reads (2 x 151 bps ...
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bioRxiv - Genomics 2021Quote: Cells were FACS sorted into 384-plate wells containing 2 µl lysis buffer (Zymo M-digestion buffer also containing proteinase K) ...
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bioRxiv - Genomics 2020Quote: ... run on 2 % agarose gel and extracted using the Zymo Gel Extraction Kit (Zymo) with final elution in 16 uL ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2×106 cells were stored in 1X DNA/RNA Shield (Zymo Research, U.S.) at -80°C for downstream molecular analysis.
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bioRxiv - Plant Biology 2023Quote: ... 2 ml of cultures were harvested and Direct-Zol RNA MiniPrep kit (Zymo Research) was used ...
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bioRxiv - Genomics 2024Quote: ... the annealed oligos were incubated with 10 mM 5-hydroxymethyl-2’-dCTP (Zymo Research), along with dGTP ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Biochemistry 2021Quote: ... Cleared supernatant was discarded and the concentrated high-speed pellet (“100,000 x g pellet”) was lysed directly in 300 μl of TRI reagent (Zymo Research, R2050-1-200). RNA extraction was performed according to instructions for the Direct-zol RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Immunology 2021Quote: ... mice were euthanized with isoflurane overdose and one lobe of lung tissue was collected in an Omni Bead ruptor tube filled with Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized using an Omni Bead Ruptor 24 (5.15 ms ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Plant Biology 2022Quote: ... Fragments were resolved with gel electrophoresis (1% agarose in 1x TAE buffer) and excised using the Zymoclean Gel DNA Recovery Kit (Zymo Research; Ref.: D4008) for elution of high-concentration ultra-pure DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Two-third of final flag elute was taken for DNase treatment using NEB DNase 1 (M0303) and RNA was purified using RNA Clean and Concentrator Kit (Zymo Research, R1015/R1017). The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: PCR products were visualized by agarose gel (1%) electrophoresis then extracted and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Lot. #213587) following manufacturer protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of genomic DNA per sample was subjected to bisulfite conversion using the EZ DNA Methylation Kit (Zymo research Cat. no. D5001) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from 2 × 106 cells using the Quick-RNA miniprep kit (Zymo) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from 2 dpf whole embryos using the Directzol RNA mini kit (Zymo). cDNA was synthesised and amplified using Thermo Fisher SuperScript II Reverse Transcriptase Kit ...
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bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
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bioRxiv - Genetics 2019Quote: Genomic DNA (2 μg) was modified by bisulfite treatment (EZ DNA methylation kit, Zymo Research) and amplified by FastStart DNA polymerase (Roche) ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (300µl) was mixed with 30µl of Proteinase K (Zymo Cat #D3001-2-20) digestion buffer and 15µl of Proteinase K in a new tube using a brief vortex step ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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bioRxiv - Genomics 2022Quote: ... then thawed and combined with 2 volumes of DNA Shield (Zymo Research, Irvine, CA, USA). Genomic DNA was extracted using a Quick DNA Miniprep Plus kit (Zymo Research ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were first grown in YPD overnight and then streaked on SC agar plates containing 1 mg/mL 5-FOA (Zymo Research, Orange, CA, USA). A single colony from an SC/5-FOA agar plate was streaked again on a new SC/5-FOA agar plate ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to extraction the samples were bead-beaten for 1 minute at 1600 RPM using ZR Bashing Bead Lysis matrix (S6012 - Zymo Research, Irvine, CA, USA). After bead beating ...
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bioRxiv - Microbiology 2021Quote: ... were then homogenised in 200 µl nuclease-free water using a tube pestle and host cells lysed by addition of 1 ml of host depletion solution (Zymo Research, Irvine, CA, USA) with a 15 min incubation at room temperature with end over end mixing ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from 1 × 105 cells using the Quick-RNA MiniPrep kit with Zymo-Spin IIICG columns (Zymo Research, Irvine, CA USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Bisulfite conversion of 1 ug genomic DNA was performed using the EZ-96 DNA Methylation Kit (Deep Well Format) (Zymo Research; Irvine, CA, USA) according to the manufacturer’s instructions (www.zymoresearch.com) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL, Zymo-IIICG = 60μL, all from Zymo Research), and then allowed to incubate on the column at 37°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... Tagmentation was carried out at 55C for 5 minutes before being stopped by the addition of 12 uL of DNA binding buffer (Zymo Research, D4004-1-L) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Double stranded TA-bridges were synthesized annealing BM-1 with non-biotinylated oligos and DNA was purified using Genomic DNA Clean & Concentrator™ (ZYMO RESEARCH, Cat.No. D4065). 30 fmol from each biotinylated oligos were used per reaction and biotinylated Epstein-Barr nuclear antigen (EBNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Following phenol/chloroform and EtOH precipitation the pellets were dissolved in 300ul 10mM Tris pH7.5 and DNA was further purified using 4 Zymo columns per sample (Zymo, D4014). Each sample was eluted in 200ul total of 10mM Tris pH7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then transferred in 4 ml tubes and processed for total RNA extraction with RNA micro kit (ZYMO REAGENT) according to company protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored in RNAlater (Invtrogen) for 24 hours at 4°C and then homogenized in TRI Reagent (Zymo Research) with a beadbeater ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of the cultures were used for total RNA extraction using the Direct-zol RNA extraction kit (Zymo Research) and TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were then harvested 4 weeks post-injection and processed using Quick-DNA midiprep plus kit (Zymo Research Cat. #D4075). For each processed tumor ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from CaCo-2 cells using Direct-zol™ RNA Microprep (ZYMO cat: R2062), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Plasmids of sorted and unsorted populations where extracted using the Zymo prep kits (Zymo, version 2) with modifications as described previously19 ...
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bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 1 ml of sediment slurry was serially diluted and 100 μL was plated in 10−4 dilution in triplicate on MB2216 agar plates using rattler beads (Zymo #S1001); colony forming units (CFU ...